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Production of beta-amylase concentrated liquid with high conversion-rate and purity

A technology with high conversion rate and production method, which is applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of insufficient conversion rate of glucose and impurity of the finished enzyme system of glucoamylase, so as to be beneficial to environmental protection and improve The effect of high equipment utilization and high purity of the enzyme system

Inactive Publication Date: 2008-12-17
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of the impurity of the finished glucoamylase enzyme system in the existing glucoamylase production process and the insufficient high conversion rate (DE value) of glucose in use, the purpose of the present invention is to improve a high conversion rate A production method of high-purity glucoamylase concentrate, thereby reducing the content of α-amylase and glucosidase in the finished glucoamylase product, thereby increasing the glucose conversion rate when the glucoamylase product is used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Embodiment 1: The primary seed tank adopts 1M 3 Tank, constant volume 0.8, ingredients in the tank according to the total weight of 800kg, including 80kg of carbon dioxide, 8kg of corn steep liquor, 8kg of ammonium sulfate, and 704kg of water; the second-level seed tank uses 5M 3 Tank, constant volume 0.8, the ingredients in the tank are based on the total weight of 4000kg, including 400kg of carbon dioxide, 80kg of bean cake powder, 0.4kg of α-amylase, 0.32kg of biotin, and 3519.28kg of water, so the second-level seed tank and the first-level seed tank The total raw materials used for seed cultivation are respectively configured in a weight ratio of 83.3% to 16.7% (i.e. 4000kg: 800kg=5: 1); the fermenter adopts 30M 3 Tank, constant volume 0.9, the total weight of the tank is 27000kg, including 4960Kg of carbon, 1080Kg of bean cake powder, 100kg of ammonium sulfate, 880Kg of liquid ammonia, 8.1kg of α-amylase, 1.62Kg of biotin, 19970.28kg of water, Therefore fermentor ...

Embodiment 2

[0007] Embodiment two: the primary seed tank adopts 1M 3 Tank, fixed volume 0.8, the total weight of the tank is 800kg, including 112kg of carbon dioxide, 16kg of ammonium sulfate, 12kg of corn steep liquor, and 660kg of water; the second-level seed tank uses 5M 3 Tank, constant volume 0.8, the ingredients in the tank are 4000kg according to the total weight, including 540kg of carbon dioxide, 140kg of bean cake powder, 0.52kg of α-amylase, 0.4kg of biotin, and 3319.08kg of water, so the second-level seed tank and the first-level seed tank The total raw materials used for seed cultivation are respectively configured in a weight ratio of 83.3% to 16.7% (i.e. 4000kg: 800kg=5: 1); the fermenter adopts 35M 3 Tank, constant volume 0.9, the total weight of the tank is 31500kg, including 8505kg carbon, 1260kg bean cake powder, 150Kg ammonium sulfate, 970kg liquid ammonia, 12.6kg α-amylase, 2.835kg biotin, 20599.565kg water, Therefore the total raw material that fermentor is used for...

Embodiment 3

[0008] Embodiment three: the primary seed tank adopts 1M 3 Tank, fixed volume 0.8, the ingredients in the tank are 800kg according to the total weight, including 104kg of carbon dioxide, 12kg of corn steep liquor, 12kg of ammonium sulfate, and 672kg of water; the secondary seed tank uses 5M 3 Tank, constant volume 0.8, the ingredients in the tank are based on the total weight of 4000kg, including 480kg of carbon dioxide, 120kg of bean cake powder, 0.6kg of α-amylase, 0.36kg of biotin, and 3399.04kg of water, so the second-level seed tank and the first-level seed tank The total raw material that is used for seed culture is to configure respectively by the weight ratio of 83.3% to 16.7% (that is 4000kg: 800kg=5: 1; Fermentation tank adopts 38M 3Tank, constant volume 0.9, the total weight of the tank is 34000kg, including 8840kg of carbon, 1310kg of bean cake powder, 150Kg of ammonium sulfate, 920kg of liquid ammonia, 12.5kg of α-amylase, 2.72kg of biotin, and 22764.78kg of water...

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Abstract

A production method of high-conversion and high-purity glucoamylase concentrate, mainly comprising: charging carbon dioxide, corn steep liquor, ammonium sulfate and water into the first-level seed tank according to the ratio; charging carbon dioxide into the second-level seed tank according to the ratio Raw material, bean cake powder, α-amylase, biotin and water; put carbon, bean cake powder, fed liquid ammonia, α-amylase, biotin and water in the fermenter according to the ratio; in the purification pretreatment tank Put sodium-based bentonite and acidified water in the medium according to the proportion; inoculate the Aspergillus niger strains into the first-level seed tank for cultivation according to the proportion; move the cultivated strains and raw materials into the second-level seed tank for expansion; The strains and raw materials are transferred to the fermenter for fermentation; next, the mature fermented liquid is put into the purification pretreatment tank for purification and pretreatment, followed by solid-liquid separation, concentration, fine filtration, sterilization and physical and chemical testing, and the finished enzyme is obtained after blending. The beneficial effect of the invention is that the purity of the glucoamylase system can be greatly improved, and the glucose conversion rate (DE value) can be obviously improved.

Description

technical field [0001] The invention relates to a production method of a hydrolase acting on glycosyl compounds, in particular to a production method of a high-conversion high-purity saccharification enzyme concentrate. Background technique [0002] As a biocatalyst, glucoamylase is mainly used in the glucoamylase process of starch in food fermentation, medicine and other industries (that is, converting starch into glucose). Starch is hydrolyzed into glucose one by one. If it is used in the alcohol industry, the starchy raw material is firstly hydrolyzed into glucose by glucoamylase. The production of alcohol is then completed by yeast fermentation, which converts the glucose into ethanol (alcohol) and carbon dioxide. At present, the main problems of glucoamylase produced in my country are: due to the unreasonable fermentation and purification process for the production of glucoamylase, the finished enzyme system of glucoamylase is not pure, especially the finished product ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/26
Inventor 李洪兵李海清张锦杰朱永明胡永明
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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