Bacillus subtilis strain and application thereof
A Bacillus subtilis, bacterial liquid technology, applied in the field of applied biology, can solve the problems of secondary air pollution, skin allergies, poor breathing, etc., and achieves stable deodorization effect, convenient transportation, and growth-promoting effects.
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Embodiment 1
[0028] Embodiment 1: Breeding of Bacillus subtilis CGMCC13714
[0029] (1) Suspend 10 mL of aquaculture sewage discharged from a pig farm in Shuangliu, Sichuan, in 90 mL of sterile distilled water, shake evenly, boil for 5 minutes, and perform gradient dilution after cooling, respectively starting from a dilution of 10 -2 、10 -4 、10 -6 、10 -8 、10 -10 0.1ml of the diluted solution was drawn, spread on the screening medium, and cultured at 35°C for 24 hours to obtain multiple colonies. The screening medium used here is beef extract peptone medium, its components include: peptone 10g, beef extract 3g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4, after 121℃, 30min high temperature sterilization .
[0030] (2) Select 5 bacterial colonies with different shapes, vigorous growth, smooth surface and sticky surface. Under the same culture conditions, these 5 bacterial strains are repeatedly streaked and cultured for more than three times to obtain pure bacterial strains, w...
Embodiment 2
[0035] Example 2: Application of Bacillus subtilis CGMCC13714 in feces deodorization
[0036] 1. Preparation of culture medium and inoculation solution
[0037] Solid activated medium: peptone 10g, beef extract 10g, yeast extract 5g, sodium chloride 5.0g, agar 15g, add water to 1000ml, pH7.0~7.4. Liquid expansion fermentation medium: peptone 5g, beef extract 5g, glucose 10g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4.
[0038] Inoculate Bacillus subtilis CGMCC13714 into the activation medium for activation and culture for 24 hours to obtain activated strains for use; inoculate the activated strains for expanded fermentation medium, shake and culture for 24 hours at 35°C and 120rmp to obtain an inoculum liquid for use.
[0039] 2. Feces deodorization treatment
[0040]Inoculate 5ml of the inoculum solution into a 1L beaker containing 500g of human feces (inoculum size 1.0%, V / W), and seal the beaker with plastic wrap; do not add the control of the bacterium solution...
Embodiment 3
[0045] Example 3: Application of Bacillus subtilis CGMCC13714 in feces deodorization
[0046] 1. Preparation of culture medium and inoculation solution
[0047] Activation medium: peptone 10g, beef extract 10g, yeast extract 5g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4. Liquid expansion fermentation medium: peptone 5g, beef extract 5g, glucose 10g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4.
[0048] Inoculate Bacillus subtilis CGMCC13714 into the activation medium for activation and culture for 24 hours to obtain activated strains for use; inoculate the activated strains for expanded fermentation medium, shake and culture for 24 hours at 30°C and 120rmp to obtain an inoculum liquid for use.
[0049] 2. Feces deodorization treatment
[0050] Inoculate 5ml of the inoculum solution into a 1L beaker containing 500g of human feces (inoculum size 1.0%, V / W), and seal the beaker with plastic wrap; do not add the control of the bacterium solution at the same t...
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