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Methods of implating mesenchymal stem cells for tissue repair and formation

a mesenchymal stem cell and tissue repair technology, applied in the field of mesenchymal stem cell isolating and transplanting mesenchymal stem cells for tissue repair or formation, can solve the problems of unable to achieve the desired goal, cells may lose their multipotency, and both methods are very difficult to be reproduced by other labs, so as to stimulate or enhance tissue repair, maintain or increase bone volume, or bone strength

Inactive Publication Date: 2005-11-10
YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes methods for isolating non-cultured mesenchymal stem cells from human cells and using them for the preparation of medications for tissue repair or formation. The methods involve contacting a human cell population with an antibody that binds to a surface molecule expressed on a mesenchymal stem cell, forming a cell-antibody complex, and recovering the mesenchymal stem cell. The isolated mesenchymal stem cells can be used to stimulate or enhance tissue repair, formation, or maintenance in a subject. The patent also provides isolated non-culture expanded human adult mesenchymal stem cells that express specific cell surface antigens.

Problems solved by technology

The culture expansion stage is extremely costly, time consuming, and in many cases, the cells may lose their multipotentiality and fail to achieve the desired goal.
Both methods are very hard to be reproduced by other labs and no studies were done to show the differentiation potential of cells before culture expansion.

Method used

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  • Methods of implating mesenchymal stem cells for tissue repair and formation
  • Methods of implating mesenchymal stem cells for tissue repair and formation
  • Methods of implating mesenchymal stem cells for tissue repair and formation

Examples

Experimental program
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Effect test

example 1

Isolation of hMSCs and Flow Cytometry:

[0076] In order to characterize the CD105+ cell population in human bone marrow (BM), human BM-derived mononuclear cells were analyzed for co-expression of CD105 antigen and other hematopoietic markers. Marker FACS analysis of freshly separated human BM mononuclear cells demonstrated that CD105 was co-expressed with other hematopoietic markers at low levels, except for CD45, which was co-expressed at higher percentages on CD105 cells (about 40%, FIG. 1C). More importantly, however, was the minimal expression of CD14 (a monocyte marker) on CD105+ cells, indicating that macrophages, the hematopoietic cell population from bone marrow with the greatest adherence to plastic, did not comprise the CD105+ cell population isolated (FIG. 1E).

[0077] CD105+ cells were isolated from human BM mononuclear cells via CD105-microbeads, then plated for expansion. Colonies of fibroblastic-like cells appeared within 7-10 days following initial plating, and were a...

example 2

In vitro osteogenic differentiation:

[0078] In order to determine the in vitro osteogenic potential of CD105+ cells, CD105+ cells were cultured following expansion (passages 3-5) under conditions inducing differentiation of mesenchymal stem cells to cells of osteogenic lineage (Pittenger et al., 1999). Significant increase in alkaline phosphatase activity was evident in induced hMSCs (with supplement), as compared to non-induced hMSCs (without supplement), during the 3 weeks of differentiation (FIG. 3A). Continual, significant increase in calcium deposition (expressed as OD at 575 nm) was observed during the differentiation period (1, 2, and 3 weeks, FIG. 3B).

example 3

Non-Culture Expanded, CD105+ hMSCs Form Cartilage and Bone in vivo:

[0079] Freshly isolated, non-culture expanded hMSCs isolated via CD105 microbead separation were transplanted into skull-defects induced in CD-1 nude mice, in order to determine their in vivo osteogenic potential. New bone formation was observed in sections processed and stained with H&E, whereas no evidence of newly formed bone was observed in similarly injured mice transplanted with collagen inserts containing CD105− cells (FIG. 4) or collagen inserts alone (data not shown).

[0080] Osteogenic differentiation of non-culture expanded hMSCs in vivo was also demonstrated via implanting the cells into NOD / SCID mice. Non-culture expanded hMSCs (1-1.5×106 cells) labeled with DiI were mixed with rhBMP2, loaded onto collagen sponges (Duragen) serving as scaffolding and transplanted under the skin of NOD / SCID mice. Cartilage and bone formation was detected in harvested implants, two weeks post-transplantation (FIGS. 5A and...

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Abstract

The invention relates to methods of isolating and implanting mesenchymal stem cells for tissue repair or formation, without prior culture expansion of the mesenchymal stem cells. In particular, the invention relates to methods of isolating mesenchymal stem cells from bone marrow, for repairing or inducing formation of bone, without prior culture expansion of the mesenchymal stem cells. The invention further relates to an isolated, non-culurally expanded human adult mesenchymal stem cell population.

Description

FIELD OF THE INVENTION [0001] The invention relates to methods of isolating and transplanting mesenchymal stem cells for tissue repair or formation, without the step of culturally expanding the mesenchymal stem cells. In another embodiment, the invention relates to methods of isolating and transplanting mesenchymal stem cells from bone marrow, for repairing or inducing formation of bone, without the step of culturally expanding the mesenchymal stem cells. BACKGROUND OF THE INVENTION [0002] Mesenchymal stem cells (MSCs) are considered one of the most promising tools for cell and cell-mediated gene therapy in skeletal repair. MSCs were shown to have the potential to differentiate into several lineages including bone (Haynesworth et al., 1992), cartilage (Mackay et al., 1998, Yoo et al., 1998), fat (Pittenger et al., 1996), tendon (Young et al., 1998), muscle, and stroma (Reviewed by Caplan et al, 2001). The main source known of MSCs in adult humans is the bone marrow compartment that ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/09A61K35/12A61K35/28A61K35/32A61K35/34A61K39/395A61K45/00A61P19/08A61P43/00C07K16/28C12N5/02C12N5/0775C12N5/10
CPCA61K35/28A61K2035/124C07K16/2842C12N5/0663C07K16/289C07K16/2896C07K16/2884A61P19/08A61P43/00
Inventor ASLAN, HADIGAZIT, DANGAZIT, ZULMA
Owner YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
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