Fermentation process of lucid ganoderma mycelium high in intracellular ganoderma triterpene content

A technology of Ganoderma lucidum mycelium and fermentation process, applied in fungi, microorganism-based methods, microorganisms, etc., can solve the problems of low content of Ganoderma lucidum triterpenes, not suitable for large-scale production, difficult to extract and purify, etc. The effect of increasing intracellular Ganoderma lucidum triterpenoid content and increasing content

Active Publication Date: 2017-03-15
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a fermentation process of ganoderma lucidum mycelia, the purpose of which is to increase the content of ganoderma lucidum triterpenes in the ganoderma lucidum mycelium by using the combination of temperature control and light treatment to stimulate, This solves the technical problems of low content of ganoderma lucidum triterpenoids in the ganoderma mycelium fermentation cells in the existing production process of ganoderma lucidum triterpenes, difficult to extract and purify, and unsuitable for large-scale production

Method used

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  • Fermentation process of lucid ganoderma mycelium high in intracellular ganoderma triterpene content
  • Fermentation process of lucid ganoderma mycelium high in intracellular ganoderma triterpene content
  • Fermentation process of lucid ganoderma mycelium high in intracellular ganoderma triterpene content

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Embodiment 1

[0048] Ganoderma lucidum mycelium fermentation process, including: Ganoderma lucidum mycelium seed cultivation stage and expanded cultivation stage:

[0049] In the seed culture stage, multi-stage seed fermentation culture is adopted, and the specific steps are as follows:

[0050] (1) Activate the mycelia of Ganoderma lucidum with PDA liquid medium, culture at 28°C to 30°C, and shaker speed 120-150r / min for 7 days to obtain the first-grade seed liquid;

[0051] Wherein, each liter of the PDA liquid medium contains 20 g of glucose and 200 g of potato juice, and is sterilized at 115° C. for 40 minutes.

[0052] (2) The primary seed liquid obtained in step (1) was inoculated in the first ganoderma lucidum seed culture medium according to the inoculum volume ratio of 1:5, and cultivated for 5 days at 28° C. to 30° C. with a shaker speed of 150 r / min. Obtain secondary seed liquid;

[0053] Each liter of the first Ganoderma lucidum seed medium contains 35g of glucose, 5g of pepto...

Embodiment 2

[0070] Ganoderma lucidum mycelium fermentation process, including: Ganoderma lucidum mycelium seed cultivation stage and expanded cultivation stage:

[0071] Seed culture stage: the steps are specifically the same as in Example 1.

[0072] Expanded cultivation stage: using light and variable temperature to stimulate the mycelia of Ganoderma lucidum, and cultivating the liquid fermentation system of the mycelia of Ganoderma lucidum for 6 days;

[0073] Among them, the Ganoderma lucidum mycelium is subjected to light treatment in conjunction with the variable temperature environment every day, as follows:

[0074] Cultivate under the condition of light for 4 hours to 16 hours, cultivate under the condition of variable temperature for 2 hours, and cultivate under the condition of 25°C to 28°C and avoid light at other times.

[0075] The culture conditions are as shown in Table 3. In the expanded culture stage, CYM medium is used. The CYM medium per liter contains 35 grams of glu...

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Abstract

The invention discloses a fermentation process of lucid ganoderma mycelium. The fermentation process includes a lucid ganoderma mycelium seed culture stage and a culture expansion state, wherein the culture expansion stage continues for 5-7 days, and lighting processing is conducted on the lucid ganoderma mycelium every day by cooperating with a variable-temperature environment and specifically comprises the following steps that culture is performed under the lighting condition for 4-16 hours, and culture is performed under the variable-temperature condition for 1-3 hours, wherein the variable-temperature condition is light shielding and high or low temperature keeping. Due to the fact that lighting and variable temperature are combined for processing, high-level accumulation of the intracellular triterpene content of lucid ganoderma is achieved, cheap, safe and easy operation can be achieved on the premise of not adding any harmless allogenic material, the intracellular ganoderma triterpene content can be remarkably increased, and the fermentation process is applicable to industrial production of ganoderma triterpenes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a fermentation process of ganoderma mycelium with high intracellular ganoderma triterpenoid content. Background technique [0002] Ganoderma lucidum triterpenoids are a class of triterpenoids found in Ganoderma lucidum, that is to say, Ganoderma lucidum triterpenes are the main chemical and medicinal components of Ganoderma lucidum. Ganoderma lucidum triterpenes contained in Ganoderma lucidum have particularly significant physiological activities. In a highly competitive society, excessive work, changes in lifestyle or environmental pollution can cause serious damage to human health, and Ganoderma lucidum triterpenes have great application value. [0003] Studies on the physiological and biochemical processes of Ganoderma lucidum have shown that triterpenoids (ganoderic acid) belong to the secondary metabolites of Ganoderma lucidum. In the research on the physiologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 胡学博李嘉伟
Owner HUAZHONG AGRI UNIV
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