Fermentation process of lucid ganoderma mycelium high in intracellular ganoderma triterpene content

Abstract

The invention discloses a fermentation process of lucid ganoderma mycelium. The fermentation process includes a lucid ganoderma mycelium seed culture stage and a culture expansion state, wherein the culture expansion stage continues for 5-7 days, and lighting processing is conducted on the lucid ganoderma mycelium every day by cooperating with a variable-temperature environment and specifically comprises the following steps that culture is performed under the lighting condition for 4-16 hours, and culture is performed under the variable-temperature condition for 1-3 hours, wherein the variable-temperature condition is light shielding and high or low temperature keeping. Due to the fact that lighting and variable temperature are combined for processing, high-level accumulation of the intracellular triterpene content of lucid ganoderma is achieved, cheap, safe and easy operation can be achieved on the premise of not adding any harmless allogenic material, the intracellular ganoderma triterpene content can be remarkably increased, and the fermentation process is applicable to industrial production of ganoderma triterpenes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a fermentation process of ganoderma mycelium with high intracellular ganoderma triterpenoid content. Background technique [0002] Ganoderma lucidum triterpenoids are a class of triterpenoids found in Ganoderma lucidum, that is to say, Ganoderma lucidum triterpenes are the main chemical and medicinal components of Ganoderma lucidum. Ganoderma lucidum triterpenes contained in Ganoderma lucidum have particularly significant physiological activities. In a highly competitive society, excessive work, changes in lifestyle or environmental pollution can cause serious damage to human health, and Ganoderma lucidum triterpenes have great application value. [0003] Studies on the physiological and biochemical processes of Ganoderma lucidum have shown that triterpenoids (ganoderic acid) belong to the secondary metabolites of Ganoderma lucidum. In the research on the physiologi...

AI Technical Summary

Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a fermentation process of ganoderma lucidum mycelia, the purpose of which is to increase the content of ganoderma lucidum triterpenes in the ganoderma lucidum mycelium by using the combination of temperature control and light treatment to stimulate, This solves the technical problems of low content of ganoderma lucidum triterpenoids in the ganoderma mycelium fermentation cells in the existing production process of ganoderma lucidum triterpenes, difficult to extract and purify, and unsuitable for large-scale production

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