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A kind of Poria cocos mycelium liquid fermentation method

A technology for liquid fermentation and mycelium, applied in fungi and other directions, can solve the problems of inability to large-scale production and low yield of Poria cocos triterpenes, and achieve the effects of easy operation and increased content.

Active Publication Date: 2020-02-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above defects or improvement needs of the prior art, the present invention provides a method for the fermentation of Poria cocos mycelia, the purpose of which is to stimulate and induce Poria cocos to synthesize Poria cocos triterpenoids by simulating the temperature change in the field, and improve the content of Poria cocos triterpene compounds. output, thereby solving the technical problem that the yield of Poria cocos triterpenoids in the prior art is low and cannot be produced on a large scale

Method used

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  • A kind of Poria cocos mycelium liquid fermentation method
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Examples

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Effect test

Embodiment 1

[0040] A method for increasing the intracellular triterpene content of Poria cocos mycelium in liquid fermentation, including the following steps:

[0041] Seed cultivation stage: Multi-level seed cultivation is used to make the poria cocos mycelium grow fully to form a good growth and adaptable poria cocos mycelium liquid fermentation system to adapt to the second stage of variable temperature treatment. The specific operation steps are as follows:

[0042] (1) Use PDA liquid medium to activate Poria cocos mycelium, culture for 7 days at 28°C to 30°C, shaker speed 120-150r / min, to obtain first-level seed liquid;

[0043] Wherein, each liter of the PDA liquid medium contains 20 g of glucose and 200 g of potato juice, and is sterilized at 121°C for 20 to 30 minutes.

[0044] (2) Inoculate the first-level seed liquid obtained in step (1) in the first Poria seed culture medium at a volume ratio of 1:5, at 28°C to 30°C, and incubate 5 at a shaker speed of 120-150r / min. Day, get the second...

Embodiment 2

[0053] A method for increasing the intracellular triterpene content of Poria cocos mycelium in liquid fermentation, including the following steps:

[0054] Seed cultivation stage: as in Example 1

[0055] Expansion of the culture stage, using variable temperature treatment, by changing the fermentation environment temperature, and simulating the influence of high and low temperature changes in the field environment on the accumulation of tuckahoe mycelium in the poria cocos mycelium fermentation to induce the poria cocos mycelium to increase the poria cocos triterpene content, the specific operation as follows:

[0056] Fermented at 4°C for 2 hours, fermented at normal temperature (28°C to 30°C) for 22 hours, and then fermented at 4°C for 2 hours, and circulated for 144 hours.

Embodiment 3

[0058] A method for increasing the intracellular triterpene content of Poria cocos mycelium in liquid fermentation, including the following steps:

[0059] Seed cultivation stage: as in Example 1

[0060] Expansion of the culture stage, using variable temperature treatment, by changing the fermentation environment temperature, and simulating the influence of high and low temperature changes in the field environment on the accumulation of tuckahoe mycelium in the poria cocos mycelium fermentation to induce the poria cocos mycelium to increase the poria cocos triterpene content, the specific operation as follows:

[0061] Fermented at 8°C for 2 hours, fermented at normal temperature (28°C to 30°C) for 22 hours, then fermented at 8°C for 2 hours, and circulated for 144 hours.

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Abstract

The invention discloses a tuckahoe mycelium liquid fermentation method which comprises the following step: in the amplification culture stage, culturing a tuckahoe mycelium liquid fermentation system at 28-30 DEG C for 5-7 days, wherein the tuckahoe mycelium liquid fermentation system is subjected to high-temperature or low-temperature treatment for 1-3 hours every day. The tuckahoe field growth environment is simulated to perform the high / low-temperature fermentation culture to implement high-level accumulation of the intracellular tuckahoe triterpene content. On the premise of not adding any allogenic material, the method is cheap and safe, is easy to operate, and can obviously enhance the intracellular tuckahoe triterpene content, thereby laying the foundation for industrial production of tuckahoe triterpene.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and more specifically, relates to a method for liquid fermentation of Poria mycelium. Background technique [0002] Poria has a very high medicinal value, and its active ingredients include β-Pachyman (β-Pachyman), which is tuckahoe polysaccharide, tuckahoe sugar, and triterpenoids Tumulasie acid (Tumulasie acid), Pmicoic acid (Pmicoic acid). At present, the acquisition of effective ingredients in Poria cocos still mainly relies on artificial cultivation and wild collection of Poria cocos. Because Poria cocos have a long growth cycle and low yield, it is difficult to produce in large quantities. [0003] However, the current liquid fermentation methods for Poria cocos mycelium mainly focus on the production of Poria cocos polysaccharides and poria cocos. There are many research reports on the extraction, purification, detection, and pharmacological effects of the medicinal components of mycelial fermentat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 胡学博李嘉伟
Owner HUAZHONG AGRI UNIV
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