A kind of culture medium of Antrodia camphorata and preparation method thereof

Abstract

The invention belongs to the technical field of fungal bioengineering, and in particular relates to a culture medium of Antrodia camphorata and a preparation method thereof. The medium provided by the present invention is composed of the following raw materials: 30-60g of cereal matrix; 0.3-0.6g of gypsum powder; 20-60ml of nutrient solution; 10-30ml of strains in shake flasks; The source supplement was soybean oil; the shake flask strain was cultured from carrots. The culture medium of the present invention selects relatively cheap grain substrates, especially wheat grains, which are suitable for the growth of Antrodia camphorata, are simple to prepare, and are suitable for enterprise-scale cultivation and production; soybean oil is selected as an exogenous additive in the present invention, which is cost-effective, and The absorption and utilization rate is high, and the content of triterpenoids in Antrodia camphorata can be increased; the shake flask nutrient solution chooses carrots instead of traditional potatoes, and adopts a three-stage culture method of constant temperature-cooling-heating, which is conducive to the growth and germination of Antrodia camphorata mycelium.

Description

technical field [0001] The invention belongs to the technical field of fungal bioengineering, and in particular relates to a culture medium of Antrodia camphorata and a preparation method thereof. Background technique [0002] Antrodia Cinnamomea belongs to the order Aphyllophyllum, Polyporaceae, and the genus Leptinosa. It is a unique and rare medicinal fungus discovered by scientists in Taiwan, China in 1990. It grows in mountainous areas at an altitude of 450-2000 meters, and only grows on the inner wall of the rotten heartwood of the trunk of the unique camphor tree more than a hundred years old in Taiwan. It is difficult to collect because the surface of the trunk is wet. At present, Taiwan calls it the most expensive wild fungus, Taiwan folks call it "the ruby ​​in the forest", and it is also called "Shenzhi" in Hong Kong and Macao. [0003] Antrodia Antrodia is rich in physiologically active ingredients, including polysaccharides, triterpenoids, superoxide dismutase,...

AI Technical Summary

Problems solved by technology

Basswood culture method can cultivate fruiting bodies of Antrodia camphorata that are similar to the active ingredients of wild Antrodia camphorata, but the cost is high and the cycle is slow, which cannot meet the market demand

The liquid culture method is an effective way to achieve industrial production of many medicinal fungi. The main advantages are short cycle and controllable quality. However, a large number of experiments by scientific research institutions have proved that the content of active ingredients in this method is low; the solid culture method has a moderate growth cycle. , but the preparation process of the space bag is complicated and the cultivation cost is high

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