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Anti-tumor active matter and its preparing method and use

A technology for anti-tumor activity and active substances, which is applied in the field of anti-tumor active substances and their preparation, and can solve problems such as no public literature reports on anti-tumor active substances.

Inactive Publication Date: 2005-10-19
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Domestic and foreign literature search shows that there is no public literature report on the use of microorganisms to transform Panax notoginseng to produce anti-tumor active substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Inoculate Monascus ruber (Monascus ruber) 3081 onto the slant of the test tube solid medium, the medium formula is wort medium, and cultivate it at 20°C for 8 days to obtain the test tube species;

[0017] 2. Inoculate the test tube seed into a solid medium in a 250ml Erlenmeyer flask (50ml per bottle). The medium formula is: 20% of Panax notoginseng fibrous root powder, 80% of water, natural pH, culture temperature 30°C, optimum 25-28 ℃, static culture for 20 days, until the hyphae are overgrown.

[0018] 3. Freeze dry the solid culture. Extract with 90% ethanol at room temperature for 2 days, concentrate under reduced pressure, and evaporate the solvent to obtain the active substance.

Embodiment 2

[0020] Basically with embodiment 1. The difference is: the temperature of the test tube solid culture is 28°C, and the time is 6 days; the formula of the solid medium is: 25% of Panax notoginseng fibrous root powder, 75% of water, the culture temperature is 25°C, and the culture is static for 18 days; % ethanol extraction at room temperature.

Embodiment 3

[0022] Basically with embodiment 1. The difference is: the temperature of the test tube solid culture is 30°C, and the time is 4 days; the formula of the solid medium is: 30% of Panax notoginseng fibrous root powder, 70% of water, the culture temperature is 20°C, and the culture is static for 15 days; % ethanol extraction at room temperature.

[0023] Two, the drug efficacy test of compound antitumor activity of the present invention

[0024] 1. Growth inhibitory effect on human tumor cells

[0025] After the cells in the logarithmic growth phase were adjusted to an appropriate concentration, they were added to a 96-well culture plate, 90ul / well. The test concentration is 100ug / ml, 3 parallel wells are set up, 10ul / well. The negative control is the same volume of culture solution, and the positive control is the anticancer drug cisplatin (DDP). At the same time, a parallel control of the corresponding concentration of DMSO solvent control and the color concentration of the...

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PUM

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Abstract

The present invention relates to active antitumor matter and its preparation process and use, and belongs to the field of microbiological medicine technology. The active matter is prepared through convenient process with Moinascus ruber 3081 of preservation number CGMCC No. 1319. The test seed is cultured in conventional malt juice culture medijm at 20-30 deg.c for 4-8 days; and the solid fermentation culture medium consists of notoginseng hairy rootlet powder 20-30 wt% and water 70-80 wt% in natural pH. Through further solid culture at 20-30 deg.c for 15-20 days, alcohol leaching with alcohol solution for 2 days, decompression concentrating and evaporating out solvent, the active matter is obtained. The active matter has high inhibiting rate to human leukaemia cell strain K562, human lung cancer strain A549, oncogene Raf1 and c-Myc latent target tyrosine phosphoesterase cdc25a and cdc25b, so that it may be used in preparing antitumor preparation.

Description

Technical field: [0001] The invention relates to an anti-tumor active substance and its preparation method and application, belonging to the technical field of microbial medicine. Background technique: [0002] Tumor (cancer) is the second largest "killer" after cardiovascular disease worldwide. According to statistics from the National Cancer Institute (NCI) in 2003, about half of American men and about one-third of women will suffer from cancer during their lives; more than 100 new cancer cases are diagnosed in the United States every year More than 500,000 people die from these diseases every year. The incidence of cancer in our country is also very alarming. There are 2 million new cancer patients and more than 1.3 million deaths every year. The total number of cancer patients in the country is estimated to be more than 5 million. With the advent of the global aging society and the increasing incidence of tumors, the market for anti-tumor drugs is also growing. Accord...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P35/00C12N1/14C12P1/02
Inventor 刘亚君张克勤
Owner YUNNAN UNIV
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