Centipede extract capable of resisting tumor activity and preparation method thereof

Abstract

The invention relates to an extract capable of resisting tumor activity, which is analyzed from centipede (a traditional Chinese medicine). The extract is obtained as follows: crushing the centipede at low temperature, conducting enzymolysis on the centipede at low temperature, separating and freeze-drying the centipede, and separating the centipede by a sephadex G-25 gel column, thereby obtaining an active ingredient which mainly comprises an effective ingredient, water-solubility micromolecule active peptide. By being tested by MTT, the extract has better anti-tumor activity. The extract avoids the defects that in the clinical application of a macromolecule protein medicine, certain allergenicity exists, and the quality control is not easy in industrialization caused by using the centipede (which is adopted as a crude drug), and has the advantages of a micromolecule activity peptide medicine, including high production efficiency, work procedure saving, quality control convenience and the like.

Description

technical field

[0001] The invention belongs to a centipede extract, in particular to a centipede extract with antitumor activity and a preparation method thereof. Background technique

[0002] Centipede is the dry body of centipede with few spiny giant centipedes. It is mainly produced in Jingzhou, Yichang, Xiaogan, Yunyang and other cities in Hubei, as well as Laohekou, Xiangfan, Jingmen, Zaoyang and other places. Warm in nature, pungent in taste, poisonous. Return liver, spleen, lung meridian. It has the functions of relieving wind and relieving spasm, attacking poison and dispelling stagnation, dredging collaterals and relieving pain. It is used for convulsions in children, convulsions, crooked mouth and eyes due to apoplexy, hemiplegia, tetanus, rheumatism, sores, scrofula, and snake bites. "Compendium of Materia Medica" records that centipedes can be used for erysipelas tumors, children's mouth, children's panic, convulsions, tetanus, crooked mouth and eyes, numbne...

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