Corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of corn continuous-cropping obstacle restoration agent
A continuous cropping obstacle and repair agent technology, applied in the field of microbial and continuous cropping obstacle repair, can solve the problems that restrict the treatment and sustainable development of corn continuous cropping obstacles, soil compaction acidification, secondary salinization, etc., and achieve the purpose of improving yield and fruit quality, Good degradation effect, the effect of promoting stress resistance
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[0014] 1. Materials and Methods
[0015] 1.1 The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.
[0016] The method for isolating Pseudomonas oryzae: wash the melon rhizome junction with running water, cut it into small pieces, soak in 75% alcohol for 1min, rinse with 0.1% mercury liter for 30s, and rinse it with sterile water for 4 times. Grind it into a slurry in a bowl, dilute the grinding solution 3 times, spread it on an inorganic salt medium, and cultivate it in an incubator at 28°C for 48 hours to obtain a strain of Pseudomonas oryzae.
[0017] Pseudomonas oryzihabitans...
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