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Degrading bacterium for autotoxic substances of rhizosphere of melon crop and application of degrading bacterium

A technology of autotoxic substances and degrading bacteria, applied in the field of microorganisms and continuous cropping obstacle repair, can solve the problems of restricting the management and sustainable development of melon crops, lack of safe and effective biological bacteria management, soil hardening and acidification, etc. The effect of good breeding ability, improving yield and fruit quality, and promoting resistance to stress

Inactive Publication Date: 2018-09-18
SHENYANG AGRI UNIV
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Problems solved by technology

[0004] At present, there are relatively few probiotics used in the treatment of continuous cropping obstacles and autotoxicity of melon crops. Most of them use fungicides and chemical fertilizers for soil treatment, which leads to problems such as soil compaction and acidification, nutrient imbalance, and secondary salinization. There is a lack of safe and effective biological Bacteria control seriously restricts the governance and sustainable development of continuous cropping obstacles of melon crops, and has become one of the bottleneck problems that urgently need to be solved in the agricultural production of melon crops

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  • Degrading bacterium for autotoxic substances of rhizosphere of melon crop and application of degrading bacterium
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  • Degrading bacterium for autotoxic substances of rhizosphere of melon crop and application of degrading bacterium

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[0022] 1. Materials and Methods

[0023] 1.1 The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.

[0024] 1.2 The method for isolating Pseudomonas oryzae: wash the melon rhizome junction with running water and cut it into small pieces, soak it in 75% alcohol for 1 min, and rinse it with 0.1% mercury chloride for 30 seconds, then rinse it with sterile water for 4 times, and put it in a sterile Grind it into a slurry in a mortar, dilute the grinding solution 3 times, spread it on an inorganic salt medium, and culture it in an incubator at 28°C for 48 hours to obtain a strain of Ps...

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Abstract

The invention discloses a degrading bacterium for autotoxic substances of the rhizosphere of a melon crop and application of the degrading bacterium. The degrading bacterium is Pseudomonas oryzihabitans, the preservation unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC); the address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number is CGMCC No. 15325; the preservation date is 29th January, 2018. The degrading bacterium can be used to prepare a microbial inoculum, and the concentration of the bacterial liquid is 2.0-3.0*10<8> cfu / ml. The microbial inoculum has good rhizosphere colonization ability and is safe and reliable when applied in a field, and has no pathogenic infection ability to intercropping crops, has a good degradation effect on the autotoxic substances in root exudates of the melon crop, promotes the anti-reverse ability of plants of the melon crop, improves the yield and fruit quality of the melon crop; the preparation method is simple, the microbial inoculum is reasonable in formula and stable in efficacy, and has an obvious alleviating effect on the autotoxic effectof the melon crop.

Description

technical field [0001] The invention relates to the field of repairing microorganisms and continuous cropping obstacles, in particular to a melon crop rhizosphere autotoxic substance degrading bacterium and application thereof. Background technique [0002] Melon crops are important economic crops in facility agriculture in my country. The repeated planting conditions in protected areas lead to great changes in soil physical and chemical properties, decreased production performance, and the accumulation of pathogenic microorganisms in the soil year by year, resulting in serious continuous cropping obstacles. A large number of studies have found that the autotoxicity of melon crops is an important cause of continuous cropping obstacles. Continuous planting leads to changes in the soil ecological environment. Plant residues, root exudates and metabolites of pathogenic microorganisms have a toxic effect on plant growth and affect seed production. Germination, plant growth, cell...

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 高增贵张一迪姚远刘限孙艳秋
Owner SHENYANG AGRI UNIV
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