Degrading bacterium for autotoxic substances of rhizosphere of melon crop and application of degrading bacterium
A technology of autotoxic substances and degrading bacteria, applied in the field of microorganisms and continuous cropping obstacle repair, can solve the problems of restricting the management and sustainable development of melon crops, lack of safe and effective biological bacteria management, soil hardening and acidification, etc. The effect of good breeding ability, improving yield and fruit quality, and promoting resistance to stress
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[0022] 1. Materials and Methods
[0023] 1.1 The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.
[0024] 1.2 The method for isolating Pseudomonas oryzae: wash the melon rhizome junction with running water and cut it into small pieces, soak it in 75% alcohol for 1 min, and rinse it with 0.1% mercury chloride for 30 seconds, then rinse it with sterile water for 4 times, and put it in a sterile Grind it into a slurry in a mortar, dilute the grinding solution 3 times, spread it on an inorganic salt medium, and culture it in an incubator at 28°C for 48 hours to obtain a strain of Ps...
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