Complex microbial inoculant used for eliminating autotoxicity of corn and applications

A compound bacterial agent, corn technology, applied in the field of microorganism and continuous cropping obstacle repair, can solve the problems of corn autotoxicity, affecting seed germination, plant growth and cell division, pathogen activity and soil microbial structure, secondary salinization, etc. Achieve the effects of increasing yield and fruit quality, improving soil physical and chemical properties, and simple preparation methods

Inactive Publication Date: 2018-09-28
SHENYANG AGRI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0002] Corn is an important field crop in northern my country. Continuous planting leads to changes in the soil ecological environment of protected areas. Plant residues, root exudates and metabolites of pathogenic microorganisms have a toxic effect on the next crop of corn, affecting seed germination, plant growth, cell Splitting, pathogen activity, and soil microbial structure, thereby triggering maize autotoxicity, resulting in continuous cropping barriers
[0003] Studies have shown that antagonistic bacteria and other means can be used to alleviate and repair the occurrence of continuous cropping obstacles caused by autotoxicity and root soil-borne diseases. At present, fungicides and chemical fertilizers are mostly used to treat soil continuous cropping obstacles on corn. Problems such as compaction acidification, nutrient imbalance, secondary salinization, etc., and the lack of safe and effective biological bacteria treatment have seriously restricted the management and sustainable development of corn continuous cropping obstacles, and have become one of the bottleneck problems that urgently need to be solved in corn agricultural production

Method used

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  • Complex microbial inoculant used for eliminating autotoxicity of corn and applications
  • Complex microbial inoculant used for eliminating autotoxicity of corn and applications
  • Complex microbial inoculant used for eliminating autotoxicity of corn and applications

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Embodiment Construction

[0014] 1. Materials and Methods

[0015] 1.1 The preparation of each component of the compound bacterial agent

[0016] (1) Weigh 10g of melon rhizosphere soil and put it into a 250mL Erlenmeyer flask, add 90mL of sterile water and shake well, and shake it on a shaker at 120r min -1 Shake for 30 minutes to fully mix the soil sample and make 10 -1 1-fold dilution; use a pipette to draw 1mL of 10 -1 Transfer the diluted solution into a sterilized test tube filled with 9mL sterile water, and make 10 -2 double dilution; then use a pipette to draw 1mL of 10 -2 Transfer the diluted solution into a sterilized test tube filled with 9mL sterile water, and finally make up 10 -3 double dilution. Melt the inorganic salt culture medium, add streptomycin and pour it into a sterilized petri dish. -3 The two-fold dilution was placed in a petri dish, and the culture solution was evenly spread with a sterile spatula. Each treatment was repeated 3 times, and cultured in a 28°C incubator fo...

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Abstract

The invention relates to a complex microbial inoculant used for eliminating the autotoxicity of corn and applications. The complex microbial inoculant is characterized by being composed of burkholderia phytofirmans and trichoderma at the ratio of (1:3) to (3:1), wherein burkholderia phytofirmans is preserved in the China General Microbiological Culture Collection Center (CGMCC, in the Institute ofMicrobiology, Chinese Academy of Sciences, located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing), and is assigned with the accession number of CGMCC No.15326, the strain number is20180101 Liao CK1, and the preservation date is Jan 29, 2018. According to the technical scheme, a seed coating method is adopted. The complex microbial inoculant is the mixture of plant endophyte and trichoderma probiotic, has good colonization capacity at the rhizosphere of corn plants, is safe and reliable, has the good degradation effect for autotoxins in the continuous cropping corn soil, obviously improves the physicochemical properties of the soil, has the obvious promoting effect for the anti-adversity capacity of the corn plants, and can improve the yield of the corn and the qualityof fruits. The complex microbial inoculant is simple in preparation method, is reasonable in formula, and can be used for treating the continuous cropping obstacles of corn.

Description

technical field [0001] The invention relates to the field of repairing microorganisms and continuous cropping obstacles, in particular to a composite bacterial agent for eliminating corn autotoxicity and its application. Background technique [0002] Corn is an important field crop in northern my country. Continuous planting leads to changes in the soil ecological environment of protected areas. Plant residues, root exudates and metabolites of pathogenic microorganisms have a toxic effect on the next crop of corn, affecting seed germination, plant growth, cell Splitting, pathogen activity and soil microbial structure, thus triggering the autotoxicity of maize, resulting in continuous cropping obstacles. [0003] Studies have shown that antagonistic bacteria and other means can be used to alleviate and repair the occurrence of continuous cropping obstacles caused by autotoxicity and root soil-borne diseases. At present, fungicides and chemical fertilizers are mostly used to tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01C1/06C12R1/01C12R1/885
CPCA01C1/06C12N1/14C12N1/20C12N1/205C12R2001/01
Inventor 高越高增贵姚远刘限李天亚
Owner SHENYANG AGRI UNIV
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