Complex microbial inoculant used for eliminating autotoxicity of corn and applications
A compound bacterial agent, corn technology, applied in the field of microorganism and continuous cropping obstacle repair, can solve the problems of corn autotoxicity, affecting seed germination, plant growth and cell division, pathogen activity and soil microbial structure, secondary salinization, etc. Achieve the effects of increasing yield and fruit quality, improving soil physical and chemical properties, and simple preparation methods
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[0014] 1. Materials and Methods
[0015] 1.1 The preparation of each component of the compound bacterial agent
[0016] (1) Weigh 10g of melon rhizosphere soil and put it into a 250mL Erlenmeyer flask, add 90mL of sterile water and shake well, and shake it on a shaker at 120r min -1 Shake for 30 minutes to fully mix the soil sample and make 10 -1 1-fold dilution; use a pipette to draw 1mL of 10 -1 Transfer the diluted solution into a sterilized test tube filled with 9mL sterile water, and make 10 -2 double dilution; then use a pipette to draw 1mL of 10 -2 Transfer the diluted solution into a sterilized test tube filled with 9mL sterile water, and finally make up 10 -3 double dilution. Melt the inorganic salt culture medium, add streptomycin and pour it into a sterilized petri dish. -3 The two-fold dilution was placed in a petri dish, and the culture solution was evenly spread with a sterile spatula. Each treatment was repeated 3 times, and cultured in a 28°C incubator fo...
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