Microbial biocontrol preparation

A technology of biocontrol bacteria and microorganisms, applied in the direction of microorganisms, biocides, plant growth regulators, etc., can solve problems such as differences in disease resistance, severe disease in cutting nurseries, poor drainage in tea gardens, etc., and achieve good control effects

Active Publication Date: 2018-10-12
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Poor drainage in tea gardens, severe disease in cutting nurseries planted too densely
Significant differences in disease resistance among cultivars

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Trichoderma longibrachiae, Bacillus amyloliquefaciens and Rhodopseudomonas palustriens were respectively inoculated into the culture solution for liquid seed culture and liquid fermentation culture to obtain Trichoderma longibrachiae fermentation broth, Bacillus amyloliquefaciens fermentation broth and Rhodopseudomonas palustris palustris. Monas fermented liquid; then Trichoderma longibrachiae fermented liquid, Bacillus amyloliquefaciens fermented liquid and Rhodopseudomonas palustris fermented liquid were mixed in proportion to obtain a mixed fermented liquid, and then appropriate amount of saihuline and chitosan were weighed and added In the mixed fermented liquid, mix evenly, make the concentration of Buihuline in the total liquid be 0.3wt%, the concentration of chitosan is 0.8wt%, and the number of live bacteria of Trichoderma longibrachiae is 3×10 7 cfu / ml, the viable count of Bacillus amyloliquefaciens is 8×10 7 cfu / ml, the viable count of Rhodopseudomonas palustr...

Embodiment 2

[0021] Trichoderma longibrachiae, Bacillus amyloliquefaciens and Rhodopseudomonas palustriens were respectively inoculated into the culture solution for liquid seed culture and liquid fermentation culture to obtain Trichoderma longibrachiae fermentation broth, Bacillus amyloliquefaciens fermentation broth and Rhodopseudomonas palustris palustris. Monas fermented liquid; then Trichoderma longibrachiae fermented liquid, Bacillus amyloliquefaciens fermented liquid and Rhodopseudomonas palustris fermented liquid were mixed in proportion to obtain a mixed fermented liquid, and then appropriate amount of saihuline and chitosan were weighed and added In the mixed fermented liquid, make the content of small Buihuline in the total liquid be that the concentration is 0.6wt%, the concentration of chitosan is 0.6wt%, and the number of living bacteria of Trichoderma longibrachiae is 8×10 7 cfu / ml, the viable count of Bacillus amyloliquefaciens is 3×10 7 cfu / ml, the viable count of Rhodopse...

Embodiment 3

[0023] Trichoderma longibrachiae, Bacillus amyloliquefaciens and Rhodopseudomonas palustriens were respectively inoculated into the culture solution for liquid seed culture and liquid fermentation culture to obtain Trichoderma longibrachiae fermentation broth, Bacillus amyloliquefaciens fermentation broth and Rhodopseudomonas palustris palustris. Monas fermented liquid; then Trichoderma longibrachiae fermented liquid, Bacillus amyloliquefaciens fermented liquid and Rhodopseudomonas palustris fermented liquid were mixed in proportion to obtain a mixed fermented liquid, and then appropriate amount of saihuline and chitosan were weighed and added In the mixed fermented liquid, make the content of small Buihuline in the total liquid be that the concentration is 0.1wt%, the concentration of chitosan is 1wt%, and the number of living bacteria of Trichoderma longibrachiae is 1×10 7 cfu / ml, the viable count of Bacillus amyloliquefaciens is 1×10 7 cfu / ml, the viable count of Rhodopseud...

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Abstract

The invention provides a microbial biocontrol preparation. The microbial biocontrol preparation is a liquid and comprises active ingredients of Trichoderma longibrachiatum and its fermentation metabolites, bacillus amyloliquefaciens and its fermentation metabolites, Rhodopseudomonas palustris and its fermentation metabolites, berberine and chitosan. The microbial biocontrol preparation has a berberine concentration of 0.1 to 0.8 wt% and a chitosan concentration of 0.5-1wt%. The total viable count of the Trichoderma longibrachiatum, bacillus amyloliquefaciens and Rhodopseudomonas palustris is greater than or equal to 5*10<7> cfu/ml. The active ingredients belong to different action mechanisms. Under the synergistic effect of the active ingredients, the microbial biocontrol preparation has good prevention effects on tea grey blight. The active ingredients are environmentally friendly and non-toxic and haves pharmacological and fertilizer dual biological regulation functions.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a microbial biocontrol preparation. Background technique [0002] Tea wheel spot disease is also called tea shoot dieback. Distributed in tea-producing regions across the country. Mainly damage leaves and new shoots. Infected young leaves, mature leaves, and old leaves of the leaves are all affected. First, small yellow-green lesions appear on the tip or edge of the leaf, and then expand into round to oval or irregular brown large lesions. Adult leaves and old leaves The lesions on the leaves have obvious concentric ring patterns. In the later stage, the center of the lesions turns off-white. When the humidity is high, small black dots arranged in ring patterns appear, which are the fruiting bodies of the pathogenic bacteria. When the young leaves are infected, they gradually turn dark brown from the tip to the edge of the leaf. The lesions are irregular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/00A01N63/02A01N63/04A01P3/00C12R1/885C12R1/07C12R1/38
CPCA01N63/00A01N63/10A01N63/30C12N1/14C12N1/20
Inventor 刘文波秦春秀缪卫国靳鹏飞林春花
Owner HAINAN UNIVERSITY
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