Pseudomonas having antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of pseudomonas

A technology of Pseudomonas and rice stripe bacteria, applied in the field of microorganisms, to achieve broad-spectrum antibacterial ability and strong inhibitory effect

Active Publication Date: 2018-12-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report on the use of Pseudomonas morganii to control rice bacterial diseases, and its biocontrol potential needs to be further developed

Method used

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  • Pseudomonas having antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of pseudomonas
  • Pseudomonas having antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of pseudomonas
  • Pseudomonas having antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of pseudomonas

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation, screening and purification of embodiment 1 Pseudomonas 923

[0041] The experimental steps are as follows:

[0042] 1. Soil source

[0043] Rhizosphere soil of unharvested paddy fields in Fengxian District, Shanghai

[0044] 2. Acquisition of strains

[0045] (1) Soil sample collection

[0046] Zig-shaped 5-point sampling method for soil sample collection: collect 200g soil samples at each point, mix the soil samples at 5 points taken according to the Z-shape evenly, take 200g by quartering method and put them into a sterilization bag as a soil sample . Three soil samples were collected from each plot as replicates. Record the time, place and type of sampling. The collected soil samples were stored in a refrigerator at 4°C for bacterial screening.

[0047] (2) Isolation of strains

[0048] Plate dilution method: Weigh 10g of each soil sample into a sterile conical conical flask, add 90mL of sterile water, then place at 200rpm, shake in a shaker at 28°...

Embodiment 2

[0053] The 16S rRNA gene identification of embodiment 2 Pseudomonas 923

[0054] Extract the genomic DNA of Pseudomonas 923, use it as a PCR template, use 27F 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R 5'-TACGGCTACCTTGTTACGACTT-3' as primers; Said reagent, carry out PCR reaction:

[0055] Table 1 Taq polymerase chain reaction system

[0056]

[0057] The basic conditions of the PCR reaction were: pre-denaturation at 94°C for 3 min, denaturation at 94°C for 30 s, annealing at 56°C for 30 s, extension at 72°C for 90 s (1 kb / min), pre-extension at 72°C for 8 min, and storage at 4°C for a total of 30 cycles. After the reaction, the PCR products were checked by 1% agarose gel electrophoresis, and the gel imager was used to detect and record the results (see attached image 3 ). Send the PCR stock solution to Bosun Biotechnology (Shanghai) Co., Ltd. for sequencing. The sequencing results were analyzed using DNA Star and compared with BLAST on the NCBI website to determine the speci...

Embodiment 3

[0059] Physiological and biochemical identification of embodiment 3 Pseudomonas mosei 923

[0060] The physiological and biochemical characteristics of Pseudomonas mosei 923 of the present invention are: it can produce fluorescent pigment, can secrete arginine dihydrolase and protease, can not hydrolyze urease and β-galactosidase; can assimilate glucose, mannose, Mannitol, N-acetyl-glucosamine and phenylacetic acid, cannot assimilate arabinose, maltose, gluconate, capric acid, adipic acid, malic acid, citric acid; can utilize dextrin, Tween 40, L-arabinose , D-fructose, α-D-glucose, D-mannitol and other 53 carbon sources, see Table 2 and Table 3.

[0061] Table 2 Physiological and biochemical characteristics of strain 923 - enzyme activity, carbon source assimilation

[0062]

[0063]

[0064] +: Positive reaction; -: Negative reaction;

[0065] Table 3 Physiological and biochemical characteristics of strain 923—acid production using carbon source

[0066]

[0067]...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a strain of pseudomonas having significant antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of the pseudomonas. The strain is isolated from rice rhizosphere soil of Fengxian District, Shanghai; the pseudomonas provided by the invention was deposited in the China Center for Type Culture Collection on May 7, 2018, the preservation name is Pseudomonas mosselii 923, and the preservation number is CCTCC No:M 2018252. The pseudomonas provided by the invention has a significant inhibitory effect on Xanthomonas oryzae, has relatively strong inhibitory capacity against other plant pathogenic bacteria and magnaporthe oryzae of Xanthomonas, and has biological control application value.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of pseudomonas with antagonistic effects on xanthomonas oryzae and rice blast fungus and its application. Background technique [0002] Rice bacterial blight and rice bacterial streak are caused by Xanthomonas oryzae pv. oryzae, Xoo and X. oryzae pv. oryzicola, Xoc, respectively. of. Xoo mainly invades through water pores or wounds at rice leaf margins, systematically harming rice vascular tissue, and causes rice bacterial leaf blight (BLB); Xoc mainly invades through stomata or wounds of rice leaves, harming Rice parenchyma cell tissue, limited by parallel veins to produce Bacterial leaf streak (BLS). BLB and BLS are important disaster-causing bacterial diseases in rice planting areas in the world, especially in rice producing areas south of the Yangtze River in my country, often resulting in a 10%-30% reduction in rice production, and in severe cases it can reach mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/38
CPCA01N63/00C12N1/205C12R2001/38
Inventor 杨瑞环邹丽芳陈功友李生樟张翠萍美丽·吾尼尔别克陈晓斌
Owner SHANGHAI JIAO TONG UNIV
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