Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis

A technology of Bacillus brevis and BBC-3, applied in application, fungicides, chemicals for biological control, etc., can solve unreported problems, increase production, promote sustainable development, and reduce product pesticides residual effect

Active Publication Date: 2015-05-27
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few researches on the biological control of broccoli fungal diseases, especially the patent literature in this area has not been reported for the time being

Method used

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  • Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis
  • Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis
  • Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis

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Experimental program
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Effect test

preparation example Construction

[0025] The preparation method of microbial preparation of the present invention comprises the following steps:

[0026] Step 1: Streak the preserved Bacillus brevis BBC-3 onto a PB medium plate and culture it at a constant temperature of 28°C for 2 days. The obtained lawn of Bacillus brevis BBC-3 was inoculated in the PB seed medium, and cultured on a shaker at 28 °C for 24 h to obtain the seed liquid.

[0027] Step 2: inoculate the seed liquid from step 1 into a large amount of fermentation medium at an inoculum volume of 10% by volume, and incubate at 28 °C for 36 h to obtain a fermentation liquid.

[0028] Step 3: After microscopic examination and counting of the fermented liquid in step 2, the concentration of the bacterial liquid is ≥ 2.0×10 9 cfu / mL, aseptically dispensed, obtained liquid preparation of Bacillus brevis microorganisms.

[0029] Step 4: Mix the liquid fermentation broth obtained in Step 2 with various substrates in a certain proportion, and then...

Embodiment 1

[0033] Example 1 Isolation, screening and preservation of Bacillus brevis BBC-3

[0034] (1) Take soil samples: Select a well-growing broccoli field, shovel off 2-3 cm of the topsoil layer in the rhizosphere of healthy broccoli plants, and take 10 g of soil close to the broccoli rhizosphere; In the kraft paper bag of bacteria, seal the mouth of the bag, and record the sampling location, environment and date.

[0035] (2) Isolation and purification of strains: The soil samples were isolated by plate dilution method on PB medium. The specific operation is as follows: Accurately weigh 10 g of soil sample, add it into a conical flask filled with 90 mL of sterile water and glass beads, shake at 200 rpm for 30 min, and make the soil sample evenly distributed to form a soil suspension with a concentration of 10 -1 ; and so on step by step 10-fold serial dilution to 10 -2 、10 -3 、10 -4 …10 -7 ; choose 10 -5 、10 -6 、10 -7 The three concentrations were kept in a constant tempe...

Embodiment 2

[0039] The mensuration of embodiment 2 Bacillus brevis BBC-3 antibacterial spectrum

[0040] Select representative broccoli gray mold, black spot, sclerotinia, black rot, Phytophthora capsici, Cucumber anthracnose, Fusarium wilt of cotton, Botrytis cinerea, Botrytis cinerea, Rhizoctonia solani, 15 kinds of plant pathogenic fungi including wheat head blight, corn spot blight, tomato early blight, leaf mold and downy mildew were used as targets, and the antibacterial spectrum of strain BBC-3 was determined by plate diffusion method. Strain BBC-3 has strong antagonistic effects on 16 kinds of plant pathogenic fungi, among which the inhibitory effect on Botrytis cinerea is the strongest, and the diameter of the inhibition zone can reach 30.23 mm, followed by black rot fungus, and the inhibition zone reaches 28.79mm.

[0041] Table 1 Inhibitory effect of Bacillus brevis BBC-3 on different plant pathogenic fungi

[0042]

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Abstract

The invention relates to an antagonistic bacterium for preventing the fungal diseases of broccoli as well as a preparation method and the application of a microbial preparation of the antagonistic bacterium, and belongs to the field of biological prevention and treatment of crop diseases. The invention provides a strain of brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622, a production method of a microbial preparation containing the brevibacillus brevis, and also the application of the brevibacillus brevis BBC-3 or the microbial preparation in preventing and treating the fungal diseases of the broccoli. The brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622 is obtained by virtue of preliminary screening and secondary screening, wherein a plate opposing test is performed for the preliminary screening and a liquid fermentation liquid is adopted for the secondary screening; the brevibacillus brevis BBC-3 is capable of effectively preventing and treating a plurality of fungal diseases of the broccoli, such as gray mold, downy mildew, stalk break, black rot and black spot, and has the prevention and treatment efficiency of above 70%; in addition, the brevibacillus brevis BBC-3 is good in environmental safety and has excellent development application prospect.

Description

technical field [0001] The present invention relates to a kind of bacillus brevis BBC-3 ( Brevibacillus brevis ) and the preparation and application of bacterial agents, belonging to the field of biological control of crop diseases. Background technique [0002] Broccoli belongs to the cruciferous family, a herbaceous plant of one or two years, and belongs to the cabbage vegetable. It is rich in vitamin A, vitamin C, phosphorus, iron, calcium and other inorganic substances, and the protein content is also very high. It is an important foreign exchange earning vegetable for export in my country. Fungal diseases are an important class of diseases on cruciferous vegetables, which are common and serious. [0003] The current control of fungal diseases of broccoli is mainly controlled by planting disease-resistant (tolerant) varieties and chemical control. As the problem of harmful pathogenic bacteria resistance is becoming more and more serious, and chemical pesticide residue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 张丽萍程辉彩习彦花尹淑丽张根伟仇艳肖崔冠慧张飞燕段普凡李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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