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Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof

A technology derived from Aeromonas hydrophila and fish, applied in the direction of bacterial medical raw materials, bacteria, antibacterial drugs, etc., can solve the problems of low immune response level, instability, and poor immune protection effect, and achieve antagonistic ability Strong, high security, broad application prospects

Inactive Publication Date: 2013-09-25
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Aeromonas hydrophila vaccine can be used to prevent the disease in fish, the immune protection effect is poor or unstable due to many serotypes of the bacteria and the low level of the body's immune response

Method used

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  • Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof
  • Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 In vitro screening of fish-derived Aeromonas hydrophila antagonistic strains

[0015] The LB solid medium was used to isolate bacteria in the intestinal tract, water environment and sediment of large freshwater fish, and the isolated bacteria were prepared into a bacterial suspension. Small paper pieces with a diameter of 6 mm were soaked and dried for bacterial screening.

[0016] Take 400uL of the indicator bacteria (Aeromonas hydrophila) and evenly spread it on the LB agar plate, soak a small piece of paper with a diameter of 6mm in the bacterial solution of Bacillus amyloliquefaciens (FFRC-S24), and wait for the plate coated with the indicator bacteria After the plate is naturally dried, take a piece of paper and stick it on it, and then put it in a constant temperature incubator at 25-30°C and incubate it face-up for 1 hour, then incubate it upside down for 48 hours. The results showed that the diameter of the inhibition zone of Bacillus amyloliquefaciens...

Embodiment 2

[0019] Bacillus amyloliquefaciens FFRC-S24 was inoculated in LB liquid medium with an inoculum volume of 20 μL / L, and cultured at 25°C for 48 hours, so that the concentration of bacteria reached 1×10 9 CFU / mL;

[0020] The components of LB liquid medium are: yeast extract 4g / L, peptone 8g / L, sodium chloride 8g / L.

[0021] Stir Bacillus amyloliquefaciens FFRC-S24 inoculated in LB liquid medium into fish feed, so that the concentration of probiotics contained in the feed is 1×10 10 CFU / KG.

Embodiment 3

[0023] Bacillus amyloliquefaciens FFRC-S24 was inoculated in LB liquid medium with an inoculum volume of 10 mL / L, and cultured at 30°C for 18 h, so that the concentration of bacteria reached 2×10 10 CFU / mL;

[0024] The components of LB liquid medium are: yeast extract 6g / L, peptone 10g / L, sodium chloride 12g / L.

[0025] Stir Bacillus amyloliquefaciens FFRC-S24 inoculated in LB liquid medium into fish feed, so that the concentration of probiotics contained in the feed is 3×10 12 CFU / KG.

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Abstract

The invention relates to a fish-sourced aeromonas hydrophila disease antagonistic strain and an application of the antagonistic strain and belongs to the field of aquaculture technology. A fish-sourced aeromonas hydrophila disease antagonistic strain is screened out, cultivated and added into a feed or a medicine as probiotics. The antagonistic strain is a novel antagonistic strain which is high in biological activity stability and high in specificity, is safe for cultured fish and environment, can be used as the probiotics strain for preventing and treating aeromonas hydrophila caused explosive hemorrhagic disease in large freshwater fish farming, and can be used as the main strain to prepare a microbial preparation.

Description

technical field [0001] The invention relates to a fish-derived Aeromonas hydrophila antagonistic strain and its application, in particular to an antagonistic antibacterial strain of Aeromonas hydrophila with high stability of antiactive components and its application, which belongs to the technical field of aquaculture. Background technique [0002] Aeromonas hydrophila ( Aeromonas hydrophila , A.hy) belongs to the Aeromonas family ( Aermonadaceae ), Aeromonas ( Aeromonas ), which exists in water, soil and aquatic organisms. Aeromonas hydrophila causes acute hemorrhagic sepsis in fish. This disease, also known as explosive epidemic, hemorrhagic disease, hemorrhagic ascites disease, ascites disease, etc., can infect major freshwater cultured species, such as grass carp, silver carp, bighead carp, carp, crucian carp, mandarin fish, herring, tilapia, eel , white crucian carp, yellow-tailed pomfret, bream, goldfish, eel and other freshwater cultured fish are the most widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/07A61K35/742
Inventor 习丙文周群兰刘亚楠梁利国谢骏朱健
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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