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31results about How to "Increased growth inhibition rate" patented technology

Streptomyces hydrogenans OsiLf-2 capable of effectively antagonizing magnaporthe oryzae in vitro

The invention discloses streptomyces hydrogenans OsiLf-2 capable of effectively antagonizing magnaporthe oryzae in vitro. The strain is collected in CGMCC (China general microbiological culture collection center), with the collection number of CGMCC No. 11673. Streptomyces hydrogenans OsiLf-2 has a good antagonistic effect on magnaporthe oryzae of at least five different physiological races in vitro. Fermentation broth of streptomyces hydrogenans OsiLf-2 also has a relatively high antagonistic effect on magnaporthe oryzae, and the antibacterial activity is very stable. Streptomyces hydrogenans OsiLf-2 and the fermentation broth have great application potential in biological prevention of rice blast, development of novel, safe, nontoxic and environment-friendly pesticides and the like.
Owner:湖南新长山农业发展股份有限公司

Addition method for enhancing algae removal effect of allelochemicals

The invention discloses an addition method for enhancing the algae removal effect of allelochemicals, and the method comprises the following steps of: A, adding allelochemicals with algal inhibiting activity into eutrophication water containing water bloom algal in a dosage of 40-60mg in a range of per cubic meter; B, adding in a manner of intermittently adding for 9-11 times from 8 a.m. to 8 p.m. in the daytime everyday and continuously adding for 5 days; and C, controlling the intermittent time for addition each time in 0.9-1.1 hours. On the premise of obviously reducing the single adding amount of the allelochemicals, the method can be used for more lastingly and effectively inhibiting the growth of the water bloom algal, preventing and controlling the outbreak of the water bloom in the eutrophication water, playing a role of flexibly, safely and effectively protecting functional waters such as water source regions, scenic waters, aquatic waters and the like, obviously enhancing the algae removal effect of the allelochemicals, realizing the efficient algae removal of the low dosage of the allelochemicals and promoting the application prospect of the allelopathy algae control technology.
Owner:INST OF AQUATIC LIFE ACAD SINICA

Sugarcane endogenous burkholderia sp. CZ08152 and application thereof

The invention discloses a sugarcane endogenous burkholderia sp. CZ08152. The burkholderia sp. CZ08152 is classified and named as burkholderia sp. CZ08152. The 16S rDNA gene sequence table of the strain is as shown in SEQ ID NO.1. The burkholderia sp. CZ08152 is preserved in China General Microbiological Culture Collection Center and the preservation number is CGMCC No. 5743. The sugarcane endogenous burkholderia sp. CZ08152 can be used for producing microbial agents and biological organic fertilizers for promoting crop growing, inhibiting plant diseases and pests and improving anti-stress of plants, thereby improving anti-stress of plants, reducing use of the fertilizer and pesticide, and protecting ecological environment. The sugarcane endogenous burkholderia sp. CZ08152 has a broad application prospect.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Preparation method and application of photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells

The invention discloses a preparation method and application of a photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells. The preparation method includes: performing amination treatment on magnetic nanoparticles Fe3O4-OA wrapped by oleic acid, and activating carboxyl on a photosensitizer; grafting the photosensitizer onto the surface of Fe3O4-OA through condensation reaction of amino and carboxyl to obtain the photosensitive magnetic nanoparticle system. The photosensitive magnetic nanoparticle system has effect of photodynamically treating breast cancer in the condition of illumination, realizes a combination of breast cancer targeting and folic acid cell targeting of a magnetic field, can effectively inhibit growth of the breast cancer cells in a targeted manner, has the characteristics of high slow release performance, stability, dispersity and uniformity and has the advantage of low toxic and side effect. The system is a novel administration path combining magnetic targeting with nano technology, is of great significance in fully utilizing drug to realize high-efficiency low-toxicity treatment effect and has good application prospect.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as target

The invention relates to the field of tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as a target, in particular to recombinant DNA carrier CpVR-FAP, recombinant adenovirus Ad-FAP and recombinant poxvirus MVA-FAP vaccines, DNA vaccines, virus vector vaccines and optimal combination of immune ways of the virus vector vaccines or application of combination of the vaccines and cyclophosphamide in preparation of anti-tumor vaccines.
Owner:JILIN UNIV

Method for preparing syngnathus acus linnaeus antineoplastic activity protein

A process is used for preparing syngnathus acus antineoplastic active protein in the medical technology field, which comprises following steps that firstly using syngnathus acus as raw material to screen after disintegrated, secondly filling in distilled water in syngnathus acus coarse powder to prepare syngnathus acus protein coarse extract, thirdly depositing protein coarse extract after pressure reduction, evaporation and condensation through adding in ammonia sulfate powder, and obtaining syngnathus acus coarse protein through dissolving with less amount of distilled water, dialyzing, condensing and drying protein coarse extract deposition, fourthly dissolving syngnathus acus coarse protein with Tris-HC1buffer solution, and then obtaining syngnathus acus antineoplastic coarse protein through conducting ion exchange chromatography and being eluted with gradient NaC1 buffer solution, fifthly dissolving syngnathus acus antineoplastic coarse protein with Tris-HC1 buffer solution, and obtaining syngnathus acus antineoplastic active protein through gel filtration chromatography. The syngnathus acus antineoplastic active protein which is prepared by the invention has high purity, and has antineoplastic activity after detected.
Owner:SHANGHAI JIAO TONG UNIV

Process for preparing anticencer active protein of thick muzzle syngnathus

The invention relates to manufacture method for anticancer active albumen in medicine technology field that the process includes: using thick sea-dragon as raw material, smashing and filtering, adding 4-12 ties alcohol water solution into the powder, vacuuming steaming and concentrating the albumen raw liquid, contacting with macroporous resin to make separating liquid, after vacuuming steaming and concentrating, adding ammiaonia sulfate powder and solving the deposit by Tris-HCl buffer solution to gain seadragon raw albuem; contacting with gel filtering medium to gain seadragon anticancer raw albumen; dissolving the raw albumen by Tris-HCl buffer solution, contacting with iron exchange medium, washing by NaCl buffer liquid. The invention has high purity, anticancer activity, and enhancing immunity.
Owner:SHANGHAI JIAO TONG UNIV

Method of utilizing biomass charcoal to inhibit phytophthora nicotianae

The invention discloses a method of utilizing biomass charcoal to inhibit phytophthora nicotianae. The method includes the following steps: firstly, configuring and adding a biomass charcoal medium; secondly, adjusting pH with the added biomass charcoal medium to be original pH of the medium; and thirdly, configuring bacteria soil. The method is simple, is convenient to use, and has a great effect to inhibit phytophthora nicotianae. Two types of biomass charcoal different in proportion are added separately or added together to an oat medium, the influence of biomass charcoal on phytophthora nicotianae is determined through determination of relevant indexes, and relevant experiments are designed to conduct mechanism verification on the basis of the property of biomass charcoal. Brown soil is selected and rice husk charcoal with different proportions is added in a potted condition to verify an effect of biomass charcoal on phytophthora nicotianae prevention. Therefore, theoretical bases are provided for reasonable applications of biomass charcoal and related research and comprehensive control and treatment of phytophthora nicotianae.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD +1

Cholic acid-alpha-amino phosphonate derivative and synthesis method thereof

The invention belongs to the field of medicine chemistry and particularly relates to a cholic acid-alpha-amino phosphonate derivative and a preparation method thereof.Cholic acid and phosphate ester serve as raw materials, and the cholic acid-alpha-amino phosphonate derivative with the following general structural formula is synthesized.This kind of compositions has good anti-tumor activity, the human hepatocellular carcinoma cell (HepG2) growth inhibition rate of some compositions is as high as 77.44%, and the advantages of the compositions are better than those of the control drug Amonafide.Please see the general structural formula in the description.
Owner:HENAN ACADEMY OF SCI CHEM RES INST CO LTD

Application of benzisoselenazole derivative combined with platinum drug to preparation of tumor treatment drugs and postoperative tumor recurrence drugs

The invention belongs to the technical field of tumor treatment, and discloses application of a benzisoselenazole derivative combined with a platinum drug to the preparation of tumor treatment drugs and postoperative tumor recurrence drugs. The benziselenazole derivative has a structure as shown in the formula A, the platinum-based anticancer drug is selected from at least one of cisplatin, carboplatin, oxaliplatin, nedaplatin, and the like. The molar ratio of the benziselenazole derivative to the platinum-based anticancer drug is (1-99): (1-99). With the combination of the benzisoselenazole derivative and the platinum drug, the dosage of platinum-based anticancer drug with high toxicity can be effectively reduced and the safety of anticancer drugs can be improved; and Bel-7402 cell apoptosis can be induced by reducing Bcl-2 / Bax protein expression ratio, and the expression of TrxR in tumor tissues can be synergistically inhibited, the proliferation rate of tumor cells after operation can be significantly reduced, and the growth inhibition rate of tumor cells after operation can be improved.
Owner:SHANGHAI YUANXI MEDICINE CORP

Fabrication method and application of cellulose membrane of a drug loading breast cancer-targeting magnetic nanoparticles

The invention discloses a fabrication method of cellulose membrane of a drug loading breast cancer-targeting magnetic nanoparticles. The method includes the following steps: 1. Grant the folic acid of optical activity by chemical modification, fix the prepared acid on the magnetic hydrogel, which then is be used to absorb hematoporphyrin monomethyl and cancer therapeutic drug, and finally obtain the tumor-targeting nano-drug; 2. Produce cellulose membrane by cultivating acetobacter xylinum and static fermentation, add the tumor-targeting nano-drug solution made in step 1 into the container for the cellulose membrane under dark condition until it's submerged, and carry out vacuum freeze drying after processed by the oscillator. The cellulose membrane of a drug loading tumor-targeting magnetic nanoparticles has high efficient in targeting the breast caner cell and suppressing its growth. At the same time, it can significantly reduce the dosage of conventional photosensitizer. In addition, the transdermal drug delivery would enhance the the drug effects on caner, realizing the controlled-release of the drug and largely lowering the toxic and side effects to healthy tissues.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Bile acid-alpha-hydroxyphosphonate derivatives and synthetic method thereof

The invention belongs to the field of pharmaceutical chemistry and particularly relates to novel bile acid-alpha-hydroxyphosphonate compounds and a preparation method thereof. Bile acid and phosphate ester are taken as raw materials, the bile acid-alpha-hydroxyphosphonate series of derivatives with a structural formula represented in the specification are synthesized, the compounds have good antitumor activity, the HepG2 growth inhibition ratio of part of the compounds is up to 75.84%, and the effect is better than that of a control drug Amonafide. Compared with traditional anti-tumor drugs such as bile acid cisplatin compounds and phosphate ester derivatives, bile acid-alpha-hydroxyphosphonate has better target selectivity and anti-tumor activity.
Owner:HENAN ACADEMY OF SCI CHEM RES INST CO LTD

Construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine

The invention discloses a fabrication method of cellulose membrane of a drug loading breast cancer-targeting magnetic nanoparticles. The method includes the following steps: 1. Grant the folic acid of optical activity by chemical modification, fix the prepared acid on the magnetic hydrogel, which then is be used to absorb hematoporphyrin monomethyl and cancer therapeutic drug, and finally obtain the tumor-targeting nano-drug; 2. Produce cellulose membrane by cultivating acetobacter xylinum and static fermentation, add the tumor-targeting nano-drug solution made in step 1 into the container for the cellulose membrane under dark condition until it's submerged, and carry out vacuum freeze drying after processed by the oscillator. The cellulose membrane of a drug loading tumor-targeting magnetic nanoparticles has high efficient in targeting the breast caner cell and suppressing its growth. At the same time, it can significantly reduce the dosage of conventional photosensitizer. In addition, the transdermal drug delivery would enhance the the drug effects on caner, realizing the controlled-release of the drug and largely lowering the toxic and side effects to healthy tissues.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Application of human ribosomal protein L23 label peptide in preparation of anti-cancer medicines

The invention discloses application of human ribosomal protein L23 label peptide to preparation of anti-cancer medicines. The amino acid sequence of the human ribosomal protein L23 label peptide is defined by SEQ ID NO:1. The human ribosomal protein L23 label peptide has time and dose dependence on growth inhibition rate of human breast cancer Bcap-37. The human ribosomal protein L23 label peptide at the concentrations of 0.2, 0.5 and 0.8 ug / ml has a good effect on growth inhibition of the human breast cancer Bcap-37, wherein the growth inhibition ratio of the human ribosomal protein L23 label peptide at the concentration of 0.5 ug / ml on the human breast cancer Bcap-37 is highest and reaches 72.29 percent.
Owner:CHINA WEST NORMAL UNIVERSITY

Preparation method of antibody coupled drug

InactiveCN109692331AIncreased growth inhibition rateThe inhibitory effect is consistentTetrapeptide ingredientsAntibody ingredientsSKBR3Heavy chain
The invention discloses a preparation method of an antibody coupled drug. The preparation method comprises following steps: mixing an anti-human ErbB2 bi-specific antibody with a reducing agent to carry out reduction reactions; after reduction, using a PBS buffer solution (pH=7, 0.01M) to dilute the reaction system by 2 to 4 times, and mixing the diluent with vc-MMAE to carry out coupling reactions to obtain the antibody coupled drug. The amino acid sequence of the anti-human ErbB2 bi-specific antibody is represented by SEQ ID No.1; and the amino acid sequence of the light chain is representedby SEQ ID No.2. The experiment results show that the antibody coupled drug and T-DM1 can inhibit the proliferation of SKBR3, NCI-N87, HCC1954, MDA-MB-453, and KPL-4, the inhibition effects are basically the same, and the JIMT-1 inhibiting effect of the antibody coupled drug is better than that of T-DM1. The preparation method has an important application value.
Owner:HEFEI HANKEMAB BIOTECH CO LTD

Process for preparing anticencer active protein of thick muzzle syngnathus

The invention relates to manufacture method for anticancer active albumen in medicine technology field that the process includes: using thick sea-dragon as raw material, smashing and filtering, adding 4-12 ties alcohol water solution into the powder, vacuuming steaming and concentrating the albumen raw liquid, contacting with macroporous resin to make separating liquid, after vacuuming steaming and concentrating, adding ammiaonia sulfate powder and solving the deposit by Tris-HCl buffer solution to gain seadragon raw albuem; contacting with gel filtering medium to gain seadragon anticancer raw albumen; dissolving the raw albumen by Tris-HCl buffer solution, contacting with iron exchange medium, washing by NaCl buffer liquid. The invention has high purity, anticancer activity, and enhancing immunity.
Owner:SHANGHAI JIAOTONG UNIV

Preparation method of corchorus olitorius seed extract and application

The invention relates to a preparation method of corchorus olitorius seed extract and application. The preparation method comprises the following steps: crushing corchorus olitorius seeds; adding petroleum ether with the weight which is 5 times as much as that of the corchorus olitorius seeds, and immersing and degreasing at a room temperature condition; after degreasing, carrying out reflowing extraction on the corchorus olitorius seeds by using an ethanol water solution with the weight which is 5-10 times as much as that of the seeds for 1-3 times, wherein each time is lasted for 1-3 hours; collecting obtained extract, namely ethanol extract of the corchorus olitorius seeds; and preserving at -20 DEG C, wherein the extract is applied to the inhibition on the proliferation of human urinary bladder cancer cells. Compared with the prior art, the inhibition on the growth of human urinary bladder cancer cell plants T24 is relatively efficient and the influences on normal urinary bladder epithelial cells are small; and the corchorus olitorius seed extract has important meanings of treating urinary bladder cancer, comprehensively utilizing jute germplasm resources of China and increasing the income of jute farmers.
Owner:TONGJI UNIV +1

Lotus seed fermented product and preparation method thereof

The invention provides a lotus seed fermented product and a preparation method thereof, and relates to the technical field of food fermentation. The preparation method of the lotus seed fermented product comprises the following steps of rehydrating lotus seeds, and performing mashing to obtain lotus seed raw pulp; performing enzymolysis on the lotus seed raw pulp to obtain enzymatic hydrolysate; inoculating the enzymatic hydrolysate with seed liquid, and performing fermentation until the content of reducing sugar in fermentation liquid is lower than 1%; and performing homogenizing and sterilization on the fermentation liquid, to obtain the lotus seed fermented product, wherein the seed liquid is obtained through the steps of inoculating culture mediums with leuconostoc mesenteroides, lactobacillus casei, lactobacillus paracasei, lactobacillus plantarum and lactobacillus acidophilus separately, performing expanded culture to obtain corresponding extended culture liquid, and performing mixing, wherein the culture mediums comprise a nitrogen source, a carbon source, inorganic salts, an accelerant, an inhibitor and water. According to the preparation method provided by the invention, functional components of the lotus seeds can be sufficiently reserved, and the lotus seed fermented product can have favorable flavor.
Owner:CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD

An endophytic actinomycete osilf-2 strain against rice blast fungus in vitro

The invention discloses a rice endophytic actinomycete capable of efficiently antagonizing blast fungus in vitro Streptomyces hydrogenans OsiLf‑2, the strain is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.11673. The bacterium can have good antagonistic effect on at least 5 kinds of rice blast fungus of different physiological races in vitro. The fermented liquid also has a strong ability to antagonize the rice blast fungus, and the antibacterial activity is very stable. The bacterium or its fermented liquid has great application potential in biological control of rice blast, development of new safe and non-toxic green pesticides and the like.
Owner:湖南新长山农业发展股份有限公司

A sugarcane endophytic Burkholderia cz08152 and its application

The invention discloses a strain of endophytic Burkholderia sp. CZ08152 in sugarcane. The classification of Burkholderia sp. CZ08152 is named as Burkholderia sp. CZ08152. The list is as shown in SEQ ID NO.1, and the depository unit is: General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the deposit number is CGMCC No.5743. The invention can be applied to the production of microbial agents and bio-organic fertilizers that can promote the growth of crops, inhibit plant diseases, and improve plant resistance to adversity stress, enhance the resistance of plants to adversity stress, and play an important role in reducing the use of chemical fertilizers and pesticides and protecting the ecological environment. significance and has broad application prospects.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

A kind of nano microsphere containing orlistat and its preparation method and application

The invention belongs to the technical field of medicines, and particularly relates to orlistat-contained nanoparticles as well as a preparation method and application thereof. According to the orlistat-contained nanoparticles as well as the preparation method and application thereof, orlistat and a copolymer are mixed to prepare the nanoparticles, so that the nanoparticles can be dissolved in anaqueous solution so as to be prepared into an oral preparation or an injection, in addition, the defect that the oral bioavailability of the orlistat is low can be overcome, the bioavailability is improved to 75% or above, the inhibition rate on hepatocellular carcinoma BEL-7402 cell strains and gastric carcinoma BGC-823 cell strains can reach 20% or above, and the orlistat-contained nanoparticlescan be widely applied to cancer therapies.
Owner:ZHONGSHAN WANHAN PHARM CO LTD

Tumor DNA Vaccine and Viral Vector Vaccine Targeting Fibroblast Activation Protein α

The invention relates to the field of tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as a target, in particular to recombinant DNA carrier CpVR-FAP, recombinant adenovirus Ad-FAP and recombinant poxvirus MVA-FAP vaccines, DNA vaccines, virus vector vaccines and optimal combination of immune ways of the virus vector vaccines or application of combination of the vaccines and cyclophosphamide in preparation of anti-tumor vaccines.
Owner:JILIN UNIV

A preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells

The invention discloses a preparation method and application of a photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells. The preparation method includes: performing amination treatment on magnetic nanoparticles Fe3O4-OA wrapped by oleic acid, and activating carboxyl on a photosensitizer; grafting the photosensitizer onto the surface of Fe3O4-OA through condensation reaction of amino and carboxyl to obtain the photosensitive magnetic nanoparticle system. The photosensitive magnetic nanoparticle system has effect of photodynamically treating breast cancer in the condition of illumination, realizes a combination of breast cancer targeting and folic acid cell targeting of a magnetic field, can effectively inhibit growth of the breast cancer cells in a targeted manner, has the characteristics of high slow release performance, stability, dispersity and uniformity and has the advantage of low toxic and side effect. The system is a novel administration path combining magnetic targeting with nano technology, is of great significance in fully utilizing drug to realize high-efficiency low-toxicity treatment effect and has good application prospect.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Cholic acid-α-aminophosphonate derivative and method for synthesizing the same

The invention belongs to the field of medicine chemistry and particularly relates to a cholic acid-alpha-amino phosphonate derivative and a preparation method thereof.Cholic acid and phosphate ester serve as raw materials, and the cholic acid-alpha-amino phosphonate derivative with the following general structural formula is synthesized.This kind of compositions has good anti-tumor activity, the human hepatocellular carcinoma cell (HepG2) growth inhibition rate of some compositions is as high as 77.44%, and the advantages of the compositions are better than those of the control drug Amonafide.Please see the general structural formula in the description.
Owner:HENAN ACADEMY OF SCI CHEM RES INST CO LTD

Preparation method of antibody coupled drug

The invention discloses a preparation method of an antibody coupled drug. The preparation method comprises the following steps: mixing an anti-human ErbB2 monoclonal antibody and a reducer for a reduction reaction; diluting a reduced reaction system with a 0.005-0.015 M PBS buffer solution, the pH of which is 7.2-7.6, for 2-4 times to obtain a diluted solution; and mixing the diluted solution withvc-MMAE for a coupling reaction to obtain the antibody coupled drug. Experiments verify that the antibody coupled drug and T-DM1 can inhibit proliferation of SKBR3, NCI-N87, HCC1954, MDA-MB453 and KPL-4 and are constant in inhibiting effect substantially. The effect of the antibody coupled drug in inhibiting JIMT-1 proliferation is obviously higher than that of T-DM1. The preparation method has important application value.
Owner:HEFEI HANKEMAB BIOTECH CO LTD

Orlistat-contained nanoparticles as well as preparation method and application thereof

The invention belongs to the technical field of medicines, and particularly relates to orlistat-contained nanoparticles as well as a preparation method and application thereof. According to the orlistat-contained nanoparticles as well as the preparation method and application thereof, orlistat and a copolymer are mixed to prepare the nanoparticles, so that the nanoparticles can be dissolved in anaqueous solution so as to be prepared into an oral preparation or an injection, in addition, the defect that the oral bioavailability of the orlistat is low can be overcome, the bioavailability is improved to 75% or above, the inhibition rate on hepatocellular carcinoma BEL-7402 cell strains and gastric carcinoma BGC-823 cell strains can reach 20% or above, and the orlistat-contained nanoparticlescan be widely applied to cancer therapies.
Owner:ZHONGSHAN WANHAN PHARM CO LTD
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