31results about How to "Increased growth inhibition rate" patented technology

The invention discloses an addition method for enhancing the algae removal effect of allelochemicals, and the method comprises the following steps of: A, adding allelochemicals with algal inhibiting activity into eutrophication water containing water bloom algal in a dosage of 40-60mg in a range of per cubic meter; B, adding in a manner of intermittently adding for 9-11 times from 8 a.m. to 8 p.m. in the daytime everyday and continuously adding for 5 days; and C, controlling the intermittent time for addition each time in 0.9-1.1 hours. On the premise of obviously reducing the single adding amount of the allelochemicals, the method can be used for more lastingly and effectively inhibiting the growth of the water bloom algal, preventing and controlling the outbreak of the water bloom in the eutrophication water, playing a role of flexibly, safely and effectively protecting functional waters such as water source regions, scenic waters, aquatic waters and the like, obviously enhancing the algae removal effect of the allelochemicals, realizing the efficient algae removal of the low dosage of the allelochemicals and promoting the application prospect of the allelopathy algae control technology.

The invention discloses a preparation method and application of a photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells. The preparation method includes: performing amination treatment on magnetic nanoparticles Fe3O4-OA wrapped by oleic acid, and activating carboxyl on a photosensitizer; grafting the photosensitizer onto the surface of Fe3O4-OA through condensation reaction of amino and carboxyl to obtain the photosensitive magnetic nanoparticle system. The photosensitive magnetic nanoparticle system has effect of photodynamically treating breast cancer in the condition of illumination, realizes a combination of breast cancer targeting and folic acid cell targeting of a magnetic field, can effectively inhibit growth of the breast cancer cells in a targeted manner, has the characteristics of high slow release performance, stability, dispersity and uniformity and has the advantage of low toxic and side effect. The system is a novel administration path combining magnetic targeting with nano technology, is of great significance in fully utilizing drug to realize high-efficiency low-toxicity treatment effect and has good application prospect.

The invention relates to the field of tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as a target, in particular to recombinant DNA carrier CpVR-FAP, recombinant adenovirus Ad-FAP and recombinant poxvirus MVA-FAP vaccines, DNA vaccines, virus vector vaccines and optimal combination of immune ways of the virus vector vaccines or application of combination of the vaccines and cyclophosphamide in preparation of anti-tumor vaccines.

A process is used for preparing syngnathus acus antineoplastic active protein in the medical technology field, which comprises following steps that firstly using syngnathus acus as raw material to screen after disintegrated, secondly filling in distilled water in syngnathus acus coarse powder to prepare syngnathus acus protein coarse extract, thirdly depositing protein coarse extract after pressure reduction, evaporation and condensation through adding in ammonia sulfate powder, and obtaining syngnathus acus coarse protein through dissolving with less amount of distilled water, dialyzing, condensing and drying protein coarse extract deposition, fourthly dissolving syngnathus acus coarse protein with Tris-HC1buffer solution, and then obtaining syngnathus acus antineoplastic coarse protein through conducting ion exchange chromatography and being eluted with gradient NaC1 buffer solution, fifthly dissolving syngnathus acus antineoplastic coarse protein with Tris-HC1 buffer solution, and obtaining syngnathus acus antineoplastic active protein through gel filtration chromatography. The syngnathus acus antineoplastic active protein which is prepared by the invention has high purity, and has antineoplastic activity after detected.

The invention discloses a method of utilizing biomass charcoal to inhibit phytophthora nicotianae. The method includes the following steps: firstly, configuring and adding a biomass charcoal medium; secondly, adjusting pH with the added biomass charcoal medium to be original pH of the medium; and thirdly, configuring bacteria soil. The method is simple, is convenient to use, and has a great effect to inhibit phytophthora nicotianae. Two types of biomass charcoal different in proportion are added separately or added together to an oat medium, the influence of biomass charcoal on phytophthora nicotianae is determined through determination of relevant indexes, and relevant experiments are designed to conduct mechanism verification on the basis of the property of biomass charcoal. Brown soil is selected and rice husk charcoal with different proportions is added in a potted condition to verify an effect of biomass charcoal on phytophthora nicotianae prevention. Therefore, theoretical bases are provided for reasonable applications of biomass charcoal and related research and comprehensive control and treatment of phytophthora nicotianae.

The invention relates to a new 2-indolinone compound and a preparation method and the usage to prepare the compounds. The general formula of the new derivatives of indolinone provided by the invention is the formula above, when in preparation, the 3-position and the 5-position of 2-indolinone are respectively introduced by amide and a carboxamide methyl sulfonyl structure to get the new derivatives. The 2-indolinone compounds of the invention have good activity of inhibition of tumor cell growth.

The invention belongs to the technical field of tumor treatment, and discloses application of a benzisoselenazole derivative combined with a platinum drug to the preparation of tumor treatment drugs and postoperative tumor recurrence drugs. The benziselenazole derivative has a structure as shown in the formula A, the platinum-based anticancer drug is selected from at least one of cisplatin, carboplatin, oxaliplatin, nedaplatin, and the like. The molar ratio of the benziselenazole derivative to the platinum-based anticancer drug is (1-99): (1-99). With the combination of the benzisoselenazole derivative and the platinum drug, the dosage of platinum-based anticancer drug with high toxicity can be effectively reduced and the safety of anticancer drugs can be improved; and Bel-7402 cell apoptosis can be induced by reducing Bcl-2/Bax protein expression ratio, and the expression of TrxR in tumor tissues can be synergistically inhibited, the proliferation rate of tumor cells after operation can be significantly reduced, and the growth inhibition rate of tumor cells after operation can be improved.

The invention discloses a fabrication method of cellulose membrane of a drug loading breast cancer-targeting magnetic nanoparticles. The method includes the following steps: 1. Grant the folic acid of optical activity by chemical modification, fix the prepared acid on the magnetic hydrogel, which then is be used to absorb hematoporphyrin monomethyl and cancer therapeutic drug, and finally obtain the tumor-targeting nano-drug; 2. Produce cellulose membrane by cultivating acetobacter xylinum and static fermentation, add the tumor-targeting nano-drug solution made in step 1 into the container for the cellulose membrane under dark condition until it's submerged, and carry out vacuum freeze drying after processed by the oscillator. The cellulose membrane of a drug loading tumor-targeting magnetic nanoparticles has high efficient in targeting the breast caner cell and suppressing its growth. At the same time, it can significantly reduce the dosage of conventional photosensitizer. In addition, the transdermal drug delivery would enhance the the drug effects on caner, realizing the controlled-release of the drug and largely lowering the toxic and side effects to healthy tissues.

The invention relates to a human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof. The human ribosomal protein RPS6 subunit anticancer peptide fragment has an amino acid sequence of ISGGNDKQGFPM, a molecular weight of 1250.41 and a PI of 5.84. The invention discloses the application of the human ribosomal protein RPS6 subunit anticancer peptide fragment in preparation of anticancer medicine. The human ribosomal protein RPS6 subunit anticancer peptide fragment has nearly no influence on growth of normal cells, has a growth inhibition effect on cancer cells, and has an obvious growth inhibition action on esophagus cancer Eca-109 cells with a growth inhibition rate up to 69.55%; a tumor inhibition rate is 59.09 by a human esophagus cancer Eca-109 nude mouse heterograft tumor model. The human ribosomal protein RPS6 subunit anticancer peptide fragment has an excellent anticancer effect and anticancer prospect.

The invention belongs to the field of pharmaceutical chemistry and particularly relates to novel bile acid-alpha-hydroxyphosphonate compounds and a preparation method thereof. Bile acid and phosphate ester are taken as raw materials, the bile acid-alpha-hydroxyphosphonate series of derivatives with a structural formula represented in the specification are synthesized, the compounds have good antitumor activity, the HepG2 growth inhibition ratio of part of the compounds is up to 75.84%, and the effect is better than that of a control drug Amonafide. Compared with traditional anti-tumor drugs such as bile acid cisplatin compounds and phosphate ester derivatives, bile acid-alpha-hydroxyphosphonate has better target selectivity and anti-tumor activity.

The invention relates to a preparation method of corchorus olitorius seed extract and application. The preparation method comprises the following steps: crushing corchorus olitorius seeds; adding petroleum ether with the weight which is 5 times as much as that of the corchorus olitorius seeds, and immersing and degreasing at a room temperature condition; after degreasing, carrying out reflowing extraction on the corchorus olitorius seeds by using an ethanol water solution with the weight which is 5-10 times as much as that of the seeds for 1-3 times, wherein each time is lasted for 1-3 hours; collecting obtained extract, namely ethanol extract of the corchorus olitorius seeds; and preserving at -20 DEG C, wherein the extract is applied to the inhibition on the proliferation of human urinary bladder cancer cells. Compared with the prior art, the inhibition on the growth of human urinary bladder cancer cell plants T24 is relatively efficient and the influences on normal urinary bladder epithelial cells are small; and the corchorus olitorius seed extract has important meanings of treating urinary bladder cancer, comprehensively utilizing jute germplasm resources of China and increasing the income of jute farmers.

The invention discloses a preparation method of an antibody coupled drug. The preparation method comprises the following steps: mixing an anti-human ErbB2 monoclonal antibody and a reducer for a reduction reaction; diluting a reduced reaction system with a 0.005-0.015 M PBS buffer solution, the pH of which is 7.2-7.6, for 2-4 times to obtain a diluted solution; and mixing the diluted solution withvc-MMAE for a coupling reaction to obtain the antibody coupled drug. Experiments verify that the antibody coupled drug and T-DM1 can inhibit proliferation of SKBR3, NCI-N87, HCC1954, MDA-MB453 and KPL-4 and are constant in inhibiting effect substantially. The effect of the antibody coupled drug in inhibiting JIMT-1 proliferation is obviously higher than that of T-DM1. The preparation method has important application value.