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Process for preparing anticencer active protein of thick muzzle syngnathus

An anti-tumor activity, crude protein technology, applied in the field of medicine, can solve the problem of no crude kiss sea dragon anti-tumor activity protein separation method and the like

Inactive Publication Date: 2006-06-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the literature search, there is no report on the technical literature related to the function of anti-tumor and immune enhancement, especially there is no report on the separation method of anti-tumor active protein of sea dragon.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) The crude Kissia chinensis medicinal material is dried at low temperature, pulverized, and passed through a 40-mesh sieve.

[0022] (2) Take 200g of crude sea dragon powder, add 800ml of aqueous solution, homogenize 3 times for 30s each time, soak in a refrigerator at 4°C for 8 hours, centrifuge at 4800rpm for 10min, remove the supernatant, and obtain the crude sea dragon protein extract.

[0023] (3) Concentrate the crude sea dragon protein extract obtained in (2) to 200ml at 40°C under reduced pressure, put it on a HZ-801 macroporous resin separation column, elute with distilled water, collect the eluted components, and concentrate under reduced pressure at 40°C to 200ml to obtain the sea dragon protein isolate.

[0024] (4) Add ammonium sulfate powder to the sea dragon protein separation solution to 20% saturation while stirring, let it stand overnight at 4°C to allow it to fully precipitate, centrifuge at 10000rpm for 10min, collect the precipitate, and wash the...

Embodiment 2

[0032] (1) The crude Kissia chinensis medicinal material is dried at low temperature, pulverized, and passed through a 20-mesh sieve.

[0033] (2) Take 200g of crude sea dragon powder, add 1400ml of 45% ethanol solution, homogenate 3 times, each time for 30s, cold soak in 4℃ refrigerator for 6 hours, centrifuge at 4800rpm for 10min, remove the supernatant, and add 1000ml of the residue again 45 % ethanol, the same operation as above, the combined two extracts are the crude extract of sea dragon protein.

[0034] (3) Concentrate the crude sea dragon protein extract obtained in (2) to 300ml at 20°C under reduced pressure, put it on a HZ-801 macroporous resin separation column, elute with distilled water, collect the eluted components, and concentrate under reduced pressure at 50°C to 250ml to obtain the sea dragon protein isolate.

[0035] (4) Add ammonium sulfate powder to the sea dragon protein separation solution to 90% saturation while stirring, let it stand overnight at 4°...

Embodiment 3

[0041] (1) The crude Kissia chinensis medicinal material is dried at low temperature, pulverized, and passed through a 20-mesh sieve.

[0042] (2) Take 200g of crude sea dragon powder, add 1600ml of 90% ethanol solution, homogenize 3 times, each time for 30s, cold soak in 4°C refrigerator for 6 hours, centrifuge at 4800rpm for 10min, remove the supernatant, which is the crude sea dragon protein Extraction.

[0043] (3) Concentrate the crude sea dragon protein extract obtained in (2) to 300ml at 60°C under reduced pressure, put it on a HZ-801 macroporous resin separation column, elute with distilled water, collect the eluted components, and concentrate under reduced pressure at 50°C to 250ml to obtain the sea dragon protein isolate.

[0044] (4) Add ammonium sulfate powder to the sea dragon protein separation solution to 55% saturation while stirring, let it stand overnight at 4°C to allow it to fully precipitate, centrifuge at 10000rpm for 10min, collect the precipitate, and ...

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PUM

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Abstract

The invention relates to manufacture method for anticancer active albumen in medicine technology field that the process includes: using thick sea-dragon as raw material, smashing and filtering, adding 4-12 ties alcohol water solution into the powder, vacuuming steaming and concentrating the albumen raw liquid, contacting with macroporous resin to make separating liquid, after vacuuming steaming and concentrating, adding ammiaonia sulfate powder and solving the deposit by Tris-HCl buffer solution to gain seadragon raw albuem; contacting with gel filtering medium to gain seadragon anticancer raw albumen; dissolving the raw albumen by Tris-HCl buffer solution, contacting with iron exchange medium, washing by NaCl buffer liquid. The invention has high purity, anticancer activity, and enhancing immunity.

Description

technical field [0001] The invention relates to a method in the technical field of medicine, in particular to a method for preparing an anti-tumor active protein of Pitchinocidae. Background technique [0002] Syngnathus (Syngnathus) is a commonly used Chinese medicinal material. The 2005 edition of the "Pharmacopoeia of the People's Republic of China" stipulates that it is the dry body of Syngnathus acus Linnaeus, Syngnathus acus Linnaeus (Gray) and Syngnathoides biaculeatus (Bloch). It is used for impotence, nocturnal emission, Accumulation of lumps in the abdomen, scrofula with sputum nucleus, injury from falls and carbuncle furuncle. In addition to the species recorded in the Pharmacopoeia, Trachyrhamphus serratus (Temminck et Schlegel) is often used as a sea dragon in many places in my country. [0003] After searching the literature of the prior art, it was found that Li Shimin et al. found in the in vitro anti-tumor activity of sea dragon, that the crude sea dragon w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14A61P35/00C07K14/435
Inventor 李晓波张朝晖秦孙星王威王梦月
Owner SHANGHAI JIAO TONG UNIV
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