Process for preparing anticencer active protein of thick muzzle syngnathus

Abstract

The invention relates to manufacture method for anticancer active albumen in medicine technology field that the process includes: using thick sea-dragon as raw material, smashing and filtering, adding 4-12 ties alcohol water solution into the powder, vacuuming steaming and concentrating the albumen raw liquid, contacting with macroporous resin to make separating liquid, after vacuuming steaming and concentrating, adding ammiaonia sulfate powder and solving the deposit by Tris-HCl buffer solution to gain seadragon raw albuem; contacting with gel filtering medium to gain seadragon anticancer raw albumen; dissolving the raw albumen by Tris-HCl buffer solution, contacting with iron exchange medium, washing by NaCl buffer liquid. The invention has high purity, anticancer activity, and enhancing immunity.

Description

technical field

[0001] The invention relates to a method in the technical field of medicine, in particular to a method for preparing an anti-tumor active protein of Pitchinocidae. Background technique

[0002] Syngnathus (Syngnathus) is a commonly used Chinese medicinal material. The 2005 edition of the "Pharmacopoeia of the People's Republic of China" stipulates that it is the dry body of Syngnathus acus Linnaeus, Syngnathus acus Linnaeus (Gray) and Syngnathoides biaculeatus (Bloch). It is used for impotence, nocturnal emission, Accumulation of lumps in the abdomen, scrofula with sputum nucleus, injury from falls and carbuncle furuncle. In addition to the species recorded in the Pharmacopoeia, Trachyrhamphus serratus (Temminck et Schlegel) is often used as a sea dragon in many places in my country.

[0003] After searching the literature of the prior art, it was found that Li Shimin et al. found in the in vitro anti-tumor activity of sea dragon, that the crude sea dragon w...