Pesticide composition for preventing and treating tobacco wildfire or angular leaf spot and application thereof

Abstract

The invention discloses a medicament composition for preventing and treating tobacco wildfire or angular leaf spot and application thereof, and belongs to the technical field of medicament compounding. In order to solve the technical problems that in the prior art, a tobacco wildfire or angular leaf spot prevention and control system is not perfect enough, and the prevention and control efficiency is low, the invention provides a pharmaceutical composition for preventing and controlling tobacco wildfire or angular leaf spot. The pesticide composition is prepared by compounding wood vinegar with any one of kasugamycin, Zhongshengmycin, DT fungicide or thiodiazole copper, and determining the optimal compounding proportion for preventing and treating tobacco wildfire and angular leaf spot, so that the purposes of improving the pesticide effect, reducing the pesticide use amount and improving the prevention and treatment efficiency of tobacco wildfire or angular leaf spot are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of compounding medicaments, and in particular relates to the technical field of compounding tobacco pesticides. Background Art

[0002] Tobacco is an important economic crop in my country's agricultural production. At present, many tobacco plant protection issues are one of the important bottlenecks restricting the development of tobacco leaves.

[0003] Tobacco wildfire or angular leaf spot is one of the important diseases that limit the development of tobacco leaves, among which:

[0004] Tobacco wildfire is a bacterial disease caused by Pseudomonas syringae pv. tabaci. It was first discovered in Virginia and North Carolina in the United States in 1917 and is now widely distributed in 26 tobacco-producing countries and regions in the world. The disease has occurred on a large scale in Yunnan, Guizhou, Henan, Shandong, Hubei, Liaoning and Jilin in my country, and has a tendency to worsen year by year, causing relatively serious economic losses. Wildfire bacteria can overwinter in the field with the remains of diseased plants, and can also survive in the roots of many other crops and weeds. Therefore, it is quite difficult to prevent the occurrence of the disease by adopting cultivation measures such as crop rotation and elimination of diseased remains in the field. Chemical control is mainly based on copper preparations and antibiotic pesticides, but copper preparations do not have a therapeutic effect. Long-term continuous use of antibiotic pesticides not only makes it easy for pathogens to develop resistance, but also harms the environment.

[0005] Tobacco angular spot disease is a bacterial disease caused by Pseudomonas syringae pv. tabaci. It can occur in all growth stages of tobacco, but is more common in the middle and late stages of field growth. All parts of the tobacco plant can be susceptible, mainly the leaves. It has spread to major tobacco-producing areas in Asia, Africa, Europe, and North and South America. In my country, the prevention and control system for tobacco angular spot disease is not yet perfect, and chemical drugs are mainly used. However, long-term use of chemical agents has increased pathogen resistance, and the concentration of applied drugs has continued to increase, resulting in a decreasing prevention and control effect.

[0006] In the actual planting process, tobacco wildfire or angular leaf spot often occurs at the same time. The probability of occurrence of these two diseases is relatively high, and there is currently no effective prevention and control method, which seriously affects the yield and quality of tobacco planting. Therefore, this field has been eager to improve the prevention and control efficiency of tobacco wildfire or angular leaf spot.

[0007] The applicant has studied the role of wood vinegar in the prevention and control of tobacco diseases. See the patent document CN116193364A "Method for obtaining the amount of trace elements added to wood vinegar based on tobacco disease prevention and control". Although the document records the combined application of wood vinegar and DT fungicide, and only has the function of preventing and controlling tobacco angular spot disease, its prevention and control effect is still not ideal. Summary of the invention

[0008] The present invention aims to solve the technical problems in the prior art that the prevention and control system of tobacco wildfire or angular spot is not perfect and the prevention and control effect is poor. The present invention provides a pharmaceutical composition for preventing and controlling tobacco wildfire or angular spot and its application.

[0009] One of the purposes of the present invention is to provide a pharmaceutical composition for preventing and treating tobacco wildfire or angular leaf spot, wherein the pharmaceutical composition is a compound of wood vinegar and any one of kasugamycin, zhongshengmycin, DT fungicide or thiophanate-methyl.

[0010] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to kasugamycin is 1:9, 2:8, 3:7 or 4:6.

[0011] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to kasugamycin is 1:9, 2:8, 3:7 or 4:6.

[0012] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to the sinobiotics is 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2 or 9:1.

[0013] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to the sinobiotics is 2:8, 3:7, 4:6, 5:5, 6:4, 7:3 or 8:2.

[0014] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to the DT fungicide is 1:9, 2:8 or 3:7.

[0015] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to the DT fungicide is 1:9, 2:8, 3:7, 4:6, 5:5 or 6:4.

[0016] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to thiophanate-methyl is 3:7, 4:6 or 5:5.

[0017] In a preferred embodiment of the present invention, when the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to thiophanate-methyl is 3:7, 4:6, 6:4, 7:3 or 8:2.

[0018] The second object of the present invention is to provide the use of the above-mentioned pharmaceutical composition in preventing and treating tobacco wildfire or angular spot disease.

[0019] Beneficial effects of the present invention:

[0020] The invention can effectively inhibit the growth of tobacco wildfire pathogens and tobacco angular spot pathogens by compounding wood vinegar with kasugamycin, zhongshengmycin, DT fungicide and thiophanate-methyl in a certain proportion.

[0021] For the prevention and control of tobacco wildfire disease, the optimal ratio of wood vinegar to kasugamycin is 4:6, and the actual growth inhibition rate of the pathogen is as high as 65.58%; the optimal ratio of wood vinegar to sinomenine is 7:3, and the actual growth inhibition rate of the pathogen is as high as 90.78%; the optimal ratio of wood vinegar to DT fungicide is 3:7, and the actual growth inhibition rate of the pathogen is as high as 43.75%; the optimal ratio of wood vinegar to thiophanate-methyl is 3:7, and the actual growth inhibition rate of the pathogen is as high as 69.93%, which effectively inhibits the growth of tobacco wildfire pathogens.

[0022] For the prevention and control of tobacco angular spot disease, the optimal ratio of wood vinegar to kasugamycin is 4:6, and the actual growth inhibition rate of the pathogen is as high as 56.37%; the optimal ratio of wood vinegar to sinomenine is 4:6, and the actual growth inhibition rate of the pathogen is as high as 64.18%; the optimal ratio of wood vinegar to DT fungicide is 2:8, and the actual growth inhibition rate of the pathogen is as high as 60.21%; the optimal ratio of wood vinegar to thiophanate-methyl is 6:4, and the actual growth inhibition rate of the pathogen is as high as 94.86%, which effectively inhibits the growth of tobacco angular spot fungus.

[0023] The pharmaceutical composition provided by the present invention improves the efficacy of a single dose, and the pharmaceutical composition achieves the purpose of improving the prevention and control effect of tobacco wildfire or angular leaf spot under the condition of reducing the use amount of a single agent. DETAILED DESCRIPTION

[0024] Those skilled in the art can refer to the content of this article and appropriately improve the process parameters. It should be particularly noted that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0025] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. The experimental methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used are all conventional materials, reagents, methods and instruments in the art unless otherwise specified, and those skilled in the art can obtain them through commercial channels.

[0026] The drugs used in this example are:

[0027] Wood vinegar: provided by the College of Resources and Environment, Northeast Agricultural University;

[0028] 2% kasugamycin aqueous solution: Hokko Chemical Industry Co., Ltd., Japan;

[0029] 3% Zhongshengmycin wettable powder: Fujian Kaili Biological Products Co., Ltd.;

[0030] 30% copper succinate wettable powder (DT fungicide): Qiqihar Siyou Chemical Co., Ltd., Heilongjiang Province;

[0031] 20% Thiophanate-copper suspension: Zhejiang Longwan Chemical Co., Ltd.

[0032] The test strains used in this example are:

[0033] In the early stage of the present invention, wildfire pathogen (109) and angular spot pathogen (217) were isolated during the investigation of tobacco wildfire disease or angular spot disease. Through molecular and morphological analysis, 109 and 217 were determined to be the pathogens causing tobacco wildfire disease and angular spot disease.

[0034] Embodiment 1:

[0035] (I) Indoor toxicity testing

[0036] In this example, the turbidity method was used to activate the wildfire bacteria (109) and angular leaf spot bacteria (217) available in the plant protection room, and then LB medium (yeast extract 5g / L, tryptone 10g / L, sodium chloride 10g / L) was used to prepare 10 7 cfu·mL -1 2 mL of bacterial solution was added to 8 mL of 25, 50, 75, 100 and 125 times diluted wood vinegar solution, and 2 mL of bacterial solution was added to 8 mL of 500, 750, 1000, 1250 and 1500 times diluted kasugamycin, zhongshengmycin, DT fungicide and thiophanate-methyl solution, respectively. The test group without wood vinegar and drug was used as blank control, and cultured at 28 ° C for 12 h, and the OD before and after culture was measured respectively. 600 The growth inhibition rate was calculated using the following formula:

[0037] Formula (1): OD600 Added value = OD after culture 600 Value-OD before culture 600 value;

[0038] Growth inhibition rate (%) = (Formula (1) - drug treatment OD 600 Added value / blank control OD 600 Added value) × 100%;

[0039] The toxicity curve was drawn with the logarithm of the treatment concentration as the horizontal axis and the inhibition rate at different concentrations converted into probability values ​​as the vertical axis. The linear regression equation, correlation coefficient and inhibition concentration EC of the single agent were calculated. 50 The results are shown in Table 1. The inhibitory effects on wildfire disease are as follows: kasugamycin> zhongshengmycin> DT fungicide> thiophanate-methyl> wood vinegar; the inhibitory effects on angular leaf spot are as follows: kasugamycin> DT fungicide> zhongshengmycin> thiophanate-methyl> wood vinegar. The EC values ​​of kasugamycin for tobacco wildfire disease or angular leaf spot disease are as follows: 50 The results show that kasugamycin has the strongest inhibitory effect on pathogens.

[0040] (II) Compounding of test agents

[0041] Based on the toxicity test results of the above single agent, according to the effects of different agents, wood vinegar was compounded with kasugamycin, zhongshengmycin, DT fungicide and thiophanate-methyl in a certain proportion, and the growth inhibition rate was measured. The results are shown in Table 1. If the toxicity ratio is <1.0, it is antagonistic; the toxicity ratio = 1, it is additive; the toxicity ratio> 1.04 is synergistic.

[0042] Table 1 Results of single-dose indoor toxicity test

[0043]

[0044] The results are shown in Table 2. For the prevention and control of tobacco wildfire, the optimal ratio of wood vinegar to kasugamycin is 4:6, the optimal ratio of wood vinegar to sinomenine is 7:3, the optimal ratio of wood vinegar to DT fungicide is 3:7, and the optimal ratio of wood vinegar to thiophanate-methyl is 3:7; for the prevention and control of tobacco angular spot, the optimal ratio of wood vinegar to kasugamycin is 4:6, the optimal ratio of wood vinegar to sinomenine is 4:6, the optimal ratio of wood vinegar to DT fungicide is 2:8, and the optimal ratio of wood vinegar to thiophanate-methyl is 6:4.

[0045] Table 2 Synergistic results of pharmaceutical compositions with different ratios

[0046]

[0047]

[0048] The contents not described in detail in the specification of the present invention are well-known technologies for those skilled in the art. Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be based on the definition of the claims.

Claims

1. A pharmaceutical composition for preventing and treating tobacco wildfire or angular spot disease, characterized in that: The pharmaceutical composition is a compound of wood vinegar and any one of kasugamycin, zhongshengmycin, DT fungicide or thiophanate-methyl.

2. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to kasugamycin is 1:9, 2:8, 3:7 or 4:

6.

3. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to kasugamycin is 1:9, 2:8, 3:7 or 4:

6.

4. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to the sinobiotics is 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2 or 9:

1.

5. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to the sinobiotics is 2:8, 3:7, 4:6, 5:5, 6:4, 7:3 or 8:

2.

6. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco wildfire disease, the ratio of the wood vinegar to the DT fungicide is 1:9, 2:8 or 3:

7.

7. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to the DT fungicide is 1:9, 2:8, 3:7, 4:6, 5:5 or 6:

4.

8. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco wildfire, the ratio of the wood vinegar to thiophanate-methyl is 3:7, 4:6 or 5:

5.

9. The pharmaceutical composition according to claim 1, characterized in that When the pharmaceutical composition is used to prevent and treat tobacco angular spot disease, the ratio of the wood vinegar to thiophanate-methyl is 3:7, 4:6, 6:4, 7:3 or 8:

2.

10. Use of the pharmaceutical composition according to any one of claims 1 to 9 in preventing and treating tobacco wildfire disease or angular leaf spot disease.

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