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Method for preparing syngnathus acus linnaeus antineoplastic activity protein

A technology of anti-tumor activity and sea dragon protein, which is applied in the field of preparation of jian sea dragon anti-tumor activity protein

Inactive Publication Date: 2008-07-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the literature search, there is no report on the separation method of the anti-tumor active protein of Jianhailong

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Jianhailong medicinal material is dried at low temperature, pulverized, and passed through a 20-mesh sieve.

[0026] (2) Take 100g of Jianhailong powder, add 800ml of distilled water, homogenate 3 times, 30s each time, soak in a refrigerator at 4°C for 8h, centrifuge at 4800rpm for 30min, remove the supernatant, add 400ml of distilled water to the residue again, do the same as above, and combine The extract is the crude extract of the sharp sea dragon protein.

[0027] (3) Concentrate the crude squidron protein extract obtained in (2) at 50° C. under reduced pressure to 300 ml to obtain the squidron protein isolate.

[0028] (4) Add ammonium sulfate powder to the sharp sea dragon protein separation solution to 85% saturation while stirring, let it stand overnight at 4°C to allow it to fully precipitate, centrifuge at 4800rpm for 30min, collect the precipitate, dissolve the precipitate with a small amount of distilled water, and then dialyze, Concentrate and freeze-...

Embodiment 2

[0036] (1) Jianhailong medicinal material is dried at low temperature, pulverized, and passed through a 20-mesh sieve.

[0037] (2) Take 100g of Jianhailong powder, add 900ml of distilled water, homogenize 3 times for 30s each time, cold soak in a refrigerator at 4°C for 12 hours, centrifuge at 4800rpm for 30min, remove the supernatant, which is the crude extract of Jianhailong protein.

[0038] (3) Concentrate the crude squidron protein extract obtained in (2) to 250 ml under reduced pressure at 45° C. to obtain the squidron protein isolate.

[0039] (4) While stirring, add ammonium sulfate powder to the sharp sea dragon protein separation solution to 70% saturation, let it stand overnight at 4°C to allow it to fully precipitate, centrifuge at 4800rpm for 30min, collect the precipitate, dissolve the precipitate with a small amount of distilled water, and then dialyze. Concentrate and freeze-dry to get the sharp sea dragon crude protein.

[0040](5) The sharp sea dragon crude...

Embodiment 3

[0044] (1) Jianhailong medicinal material is dried at low temperature, pulverized, and passed through a 20-mesh sieve.

[0045] (2) Take 100g of Jianhailong powder, add 600ml of distilled water, homogenize 3 times, each time for 30s, cold soak in 4℃ refrigerator for 16 hours, centrifuge at 4800rpm for 30min, remove the supernatant, which is the crude extract of Jianhailong protein.

[0046] (3) Concentrate the crude squidron protein extract obtained in (2) at 40° C. under reduced pressure to 200 ml to obtain the squidron protein isolate.

[0047] (4) Add ammonium sulfate powder to the sharp sea dragon protein separation solution to 55% saturation while stirring, let it stand overnight at 4°C to allow it to fully precipitate, centrifuge at 4800rpm for 30min, collect the precipitate, dissolve the precipitate with a small amount of distilled water, and then dialyze, Concentrate and freeze-dry to get the sharp sea dragon crude protein.

[0048] (5) The sharp sea dragon crude prot...

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PUM

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Abstract

A process is used for preparing syngnathus acus antineoplastic active protein in the medical technology field, which comprises following steps that firstly using syngnathus acus as raw material to screen after disintegrated, secondly filling in distilled water in syngnathus acus coarse powder to prepare syngnathus acus protein coarse extract, thirdly depositing protein coarse extract after pressure reduction, evaporation and condensation through adding in ammonia sulfate powder, and obtaining syngnathus acus coarse protein through dissolving with less amount of distilled water, dialyzing, condensing and drying protein coarse extract deposition, fourthly dissolving syngnathus acus coarse protein with Tris-HC1buffer solution, and then obtaining syngnathus acus antineoplastic coarse protein through conducting ion exchange chromatography and being eluted with gradient NaC1 buffer solution, fifthly dissolving syngnathus acus antineoplastic coarse protein with Tris-HC1 buffer solution, and obtaining syngnathus acus antineoplastic active protein through gel filtration chromatography. The syngnathus acus antineoplastic active protein which is prepared by the invention has high purity, and has antineoplastic activity after detected.

Description

technical field [0001] The invention relates to a method in the technical field of medicine, in particular to a method for preparing an anti-tumor active protein of Sea Dragon. Background technique [0002] Sea dragon is a commonly used Chinese medicinal material. "Pharmacopoeia of the People's Republic of China" (2005 edition) stipulates that sea dragon is the dry body of Syngnathus acus Linnaeus, Solenognathus hardwickii (Gray) and Syngnathoides biaculeatus (Bloch), which are used for impotence, spermatorrhea, Accumulation of lumps in the abdomen, scrofula and phlegm nucleus, injury from falls and carbuncle furuncle. In recent years, the research on active proteins and peptides of animal medicinal materials has become increasingly active, especially anti-tumor proteins and peptides have become the focus of research and development. Due to the differences between marine organisms and inland organisms, protein components from marine medicinal animals have more development a...

Claims

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Application Information

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IPC IPC(8): C07K1/14A61P35/00
Inventor 李晓波王梦月聂玉晓鞠培俊
Owner SHANGHAI JIAO TONG UNIV
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