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A preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells

A technology of magnetic nanoparticles and breast cancer cells, which is used in pharmaceutical formulations, wave energy or particle radiation treatment materials, medical preparations with non-active ingredients, etc.

Active Publication Date: 2019-08-06
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the combined effect of natural photosensitizers and artificial photosensitizers has rarely been reported.

Method used

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  • A preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells
  • A preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells
  • A preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Synthesis and Characterization of Nanoparticles

[0058] 1. The schematic diagram of the synthesis of nanoparticles is attached figure 1 As shown, the specific method is as follows:

[0059] (1) Magnetic nanoparticles coated with oleic acid (Fe 3 o 4 -OA) synthesis

[0060] FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 O, add ultra-pure water, put it in an oil bath, fill it with nitrogen for protection, and stir at 60°C for 30 min; take NaOH and add it into ultra-pure water, fully stir and dissolve at 60°C, and add it to the above iron salt solution; the mixed solution After reacting for 10 min, heat up to 70 °C, add HCl solution, and adjust the pH to 3; add oleic acid to the reacted solution, stir for 3 h, wash with absolute ethanol and acetone under the assistance of a magnet, and finally deionized water Dry for 40 h after washing.

[0061] (2) Modification of amino groups on magnetic nanoparticles (Fe 3 o 4 -OA-NH 2 )

[0062] Mix 100 mL of ethanol and 25 mL...

Embodiment 2

[0080] Example 2 Application of Nanoparticles Inhibiting Breast Cancer in Vitro

[0081] 1. Morphological observation

[0082] Take out the cultured cells from the incubator, digest with trypsin for 2min, add 4ml of culture medium and blow the cells. Add 3ml of cell suspension to a centrifuge tube, add 11mL of culture medium, and blow the cells to make the cells evenly suspended. Use a pipette gun to draw 100 μL of the cell suspension and add it to a 24-well plate to allow the cells to adhere to the wall and culture for 6 hours. The synthesized nanomaterials were taken out under dark conditions and adsorbed by a magnet to precipitate the nanomaterials to the bottom of the test tube. Aspirate the supernatant, wash the nanomaterials with PBS, and absorb them with a magnet to precipitate the nanomaterials to the bottom of the test tube. Aspirate the supernatant, add PBS to mix the nanomaterials. Take out the cultured cells, suck off the cell waste liquid, add PBS to wash twic...

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Abstract

The invention discloses a preparation method and application of a photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells. The preparation method includes: performing amination treatment on magnetic nanoparticles Fe3O4-OA wrapped by oleic acid, and activating carboxyl on a photosensitizer; grafting the photosensitizer onto the surface of Fe3O4-OA through condensation reaction of amino and carboxyl to obtain the photosensitive magnetic nanoparticle system. The photosensitive magnetic nanoparticle system has effect of photodynamically treating breast cancer in the condition of illumination, realizes a combination of breast cancer targeting and folic acid cell targeting of a magnetic field, can effectively inhibit growth of the breast cancer cells in a targeted manner, has the characteristics of high slow release performance, stability, dispersity and uniformity and has the advantage of low toxic and side effect. The system is a novel administration path combining magnetic targeting with nano technology, is of great significance in fully utilizing drug to realize high-efficiency low-toxicity treatment effect and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials. More specifically, it relates to a preparation method and application of a photosensitive magnetic nanoparticle system that inhibits the growth of breast cancer cells. Background technique [0002] Breast cancer is one of the most common malignant tumors in women worldwide, and it has gradually ranked first in female malignant tumors in recent years. According to relevant statistics, the average annual growth rate of the incidence of breast cancer in many large cities in my country is much higher than the world average growth rate. At the same time, the mortality rate of breast cancer in my country is also slowly rising, and the age of onset of breast cancer tends to be younger. The median age of onset is 48 years old, 10 years earlier than in Western countries. Health poses a serious threat. [0003] At present, the treatment of breast cancer is mainly based on surgery, combined w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K47/69A61K41/00A61P35/00
CPCA61K41/0052A61K41/0071A61K41/0076
Inventor 关燕清杜世伟张令坤
Owner SOUTH CHINA NORMAL UNIVERSITY
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