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Construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine

A cellulose membrane, magnetic nanotechnology, applied in the field of biomedicine, can solve problems such as drug resistance, metastasis, and patient recurrence

Active Publication Date: 2020-01-14
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of breast cancer is mainly based on surgery, combined with postoperative radiotherapy and chemotherapy, endocrine therapy and targeted therapy. Adverse reactions and drug resistance have also become obstacles to the treatment of many patients

Method used

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  • Construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine
  • Construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine
  • Construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1 prepares composite cellulose film

[0097] 1 Materials, reagents and instruments

[0098] 1.1 Cell lines

[0099] Human breast cancer cells (MCF-7 cell line) were provided by Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences and subcultured in our laboratory.

[0100] 1.2 Main reagents and instruments

[0101] Iron ferric oxide nanoparticles and hydrogel: synthesized in the early stage of this laboratory; hematoporphyrin monomethyl ether: purchased from Guangzhou Weijia Biotechnology Co., Ltd.; pancreatin and low-sugar DMEM medium are all products of GIBCOBRL; Bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; 96-well polystyrene tissue culture plates were products of Corning, USA.

[0102] Nikon microscope, Japan Olympus optical inverted microscope, Sigma32184 high-speed refrigerated centrifuge, Thermo CO 2 Incubator, Jiangsu Jintan Medical Instrument Factory 78-1 magnetic stirrer, HV-85 autoclave,...

Embodiment 2

[0159] Example 2 Experiment of Inhibiting the Growth of Breast Cancer MCF7 Cells in Vitro

[0160] 1. Cell culture

[0161] Human breast cancer cells (MCF-7 cell line) were provided by the Shenzhen Institutes of Advanced Technology, University of Chinese Academy of Sciences. After the cells were cultured to 80% in the culture flask, 1×10 4 The density per well was inoculated on a 24-well plate and cultured for 12, 24, 36 for subsequent experiments. Other cell culture conditions are: high-glucose DMEM medium containing 10% newborn calf serum, 37°C, 5.0% CO 2 .

[0162] 2. Cellulose membrane-loaded nanoparticles inhibited the growth of MCF7

[0163] (1) Cell culture

[0164] MCF7 cells in good growth condition were cultured in DMEM with high glucose and serum. When the cells grew to 70-80%, they were digested and plated (6-well plate) at a density of about 1.0×10 5 pcs / hole.

[0165] (2) Experimental magnetic field and laser

[0166] Experimental magnetic field (M): abou...

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Abstract

The invention discloses a fabrication method of cellulose membrane of a drug loading breast cancer-targeting magnetic nanoparticles. The method includes the following steps: 1. Grant the folic acid of optical activity by chemical modification, fix the prepared acid on the magnetic hydrogel, which then is be used to absorb hematoporphyrin monomethyl and cancer therapeutic drug, and finally obtain the tumor-targeting nano-drug; 2. Produce cellulose membrane by cultivating acetobacter xylinum and static fermentation, add the tumor-targeting nano-drug solution made in step 1 into the container for the cellulose membrane under dark condition until it's submerged, and carry out vacuum freeze drying after processed by the oscillator. The cellulose membrane of a drug loading tumor-targeting magnetic nanoparticles has high efficient in targeting the breast caner cell and suppressing its growth. At the same time, it can significantly reduce the dosage of conventional photosensitizer. In addition, the transdermal drug delivery would enhance the the drug effects on caner, realizing the controlled-release of the drug and largely lowering the toxic and side effects to healthy tissues.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to a construction method and application of a cellulose membrane loaded with breast cancer-targeted magnetic nanomedicine. Background technique [0002] Breast cancer is one of the most common malignant tumors in women worldwide, and it has gradually ranked first in female malignant tumors in recent years. According to relevant statistics, the incidence of breast cancer in many large cities in my country is increasing year by year with a rapid growth trend, and the average annual growth rate is much higher than the world average growth rate. At the same time, the mortality rate of breast cancer in my country is also slowly rising, and the age of onset of breast cancer tends to be younger. The median age of onset is 48 years old, 10 years earlier than in Western countries. Health poses a serious threat. [0003] At present, the treatment of breast cancer is main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K31/704A61K47/02A61K47/22A61K47/38A61P35/00
CPCA61K9/0009A61K31/704A61K41/0071A61K47/02A61K47/22A61K47/38A61K2300/00
Inventor 关燕清张令坤
Owner SOUTH CHINA NORMAL UNIVERSITY
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