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Preparation method of corchorus olitorius seed extract and application

A technology of extract and jute, which is applied in the field of medicine, can solve the problems of bladder cancer cells from jute seed extracts that have not yet been seen, and achieve good development and application prospects, increase the income of hemp farmers, and increase the growth inhibition rate.

Inactive Publication Date: 2015-08-26
TONGJI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the literature search, so far there is no report on the use of jute seed extract of long fruit species to inhibit the proliferation of bladder cancer cells and related diseases

Method used

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  • Preparation method of corchorus olitorius seed extract and application
  • Preparation method of corchorus olitorius seed extract and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of ethanol extract of jute seeds

[0028] Take 100g of long-fruited jute seeds, crush them, add 500ml of petroleum ether to degrease and degrease at room temperature for 48 hours, then use 1000ml of 70% ethanol aqueous solution, seal and soak at room temperature for 2 hours, heat in a water bath to reflux, and extract by reflux for 3 hours each time. 2 times. The ethanol extracts collected twice were filtered and then concentrated with a rotary evaporator to obtain extracts. Collect the extract (that is, the ethanol extract of jute seeds) and store at -20°C for future use.

Embodiment 2

[0030] Inhibitory effect of ethanol extract of jute seeds of Changguo species on the proliferation of human bladder cancer cells

[0031] Cells in the logarithmic growth phase: Human bladder cancer cells T24 (purchased from the Cell Bank of the Chinese Academy of Sciences) were seeded in a 96-well plate at a concentration of 10,000 cells per well.

[0032] After overnight attachment, 0.1, 0.5, 1, 5 and 10 ug / ml ethanol extracts of jute seeds were administered, and 5, 10, 50 and 100 ug / ml mitomycin was used as a positive control. For each treatment, 6 replicate wells were set up, and the experiment was repeated 3 times.

[0033] After 24 hours of administration, the proliferation and growth rate of human bladder cancer T24 cells was detected by MTT method, and the IC50 value was calculated.

[0034] figure 1 It is the IC50 value after the T24 cells were acted on by the long fruit seed extract and mitomycin for 24 hours, as shown in the figure, after acting on the T24 cells fo...

Embodiment 3

[0037] The preparation method of long fruit seed jute seed extract adopts the following steps:

[0038] (1) Pulverize wild long-fruited jute seeds, add sherwood oil 5 times of its weight, extract and degrease at room temperature for 48h;

[0039] (2) After degreasing, utilize 5 times of weight, and volume fraction is 90% ethanol aqueous solution airtightly soaks the jute seed after degreasing at room temperature 2h, then is heated to reflux temperature in water bath and carries out reflux extraction 1 time, and extraction time is 3h , collect the obtained extract, which is the ethanol extract of jute seeds, and store at -20°C.

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Abstract

The invention relates to a preparation method of corchorus olitorius seed extract and application. The preparation method comprises the following steps: crushing corchorus olitorius seeds; adding petroleum ether with the weight which is 5 times as much as that of the corchorus olitorius seeds, and immersing and degreasing at a room temperature condition; after degreasing, carrying out reflowing extraction on the corchorus olitorius seeds by using an ethanol water solution with the weight which is 5-10 times as much as that of the seeds for 1-3 times, wherein each time is lasted for 1-3 hours; collecting obtained extract, namely ethanol extract of the corchorus olitorius seeds; and preserving at -20 DEG C, wherein the extract is applied to the inhibition on the proliferation of human urinary bladder cancer cells. Compared with the prior art, the inhibition on the growth of human urinary bladder cancer cell plants T24 is relatively efficient and the influences on normal urinary bladder epithelial cells are small; and the corchorus olitorius seed extract has important meanings of treating urinary bladder cancer, comprehensively utilizing jute germplasm resources of China and increasing the income of jute farmers.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method and application of a long-fruited jute seed extract. Background technique [0002] Bladder cancer is the most common malignant tumor of the urinary system, and its incidence rate ranks ninth among malignant tumors in the world and ranks first among malignant tumors of the urinary system in my country. Although 70-80% of primary bladder cancers are non-invasive (also known as superficial), they are prone to recurrence after surgery, with a recurrence rate as high as 70%, and about 15% of patients develop invasive bladder cancer. At present, regular intravesical infusion of mitomycin (MMC) and other chemotherapeutic drugs after transurethral resection of bladder tumor (TUR-BT) is mainly used clinically to prevent the recurrence and progression of non-invasive bladder cancer, but the effective rate is low (only 30-50%) and problems such as large t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61P13/10A61K131/00
Inventor 朱云国程舟李珊杨晓伶李德芳龚友才肖爱平
Owner TONGJI UNIV
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