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Method for preparing extract of corchorus capsularis seeds and application of extract

An extract and technology of jute, which is applied in the field of medicine, can solve the problems that have not been reported on bladder cancer cells from jute seed extract of round fruit species, achieve good development and application prospects, have little impact, and increase the income of hemp farmers

Inactive Publication Date: 2015-09-09
TONGJI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the literature search, so far there is no report on the use of the extract of jute seeds of the fruit of the fruit for inhibiting the proliferation of bladder cancer cells and related diseases.

Method used

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  • Method for preparing extract of corchorus capsularis seeds and application of extract
  • Method for preparing extract of corchorus capsularis seeds and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of ethanol extract of jute seeds

[0027] Take 100g of jute seeds, crush them, add 500ml of petroleum ether to degrease at room temperature for 48h, then use 1000ml of 70% ethanol aqueous solution, soak for 2h at room temperature, heat in a water bath to reflux, extract for 3h each time, and reflux Extract 2 times. The ethanol extracts collected twice were filtered and then concentrated with a rotary evaporator to obtain extracts. Collect the extract (that is, the ethanol extract of the jute seeds of the genus Cymbidium) and store it at -20°C for future use.

Embodiment 2

[0029] Inhibitory effect of ethanol extracts of jute seeds from Cymbidium spp. on the proliferation of human bladder cancer cells

[0030] Cells in the logarithmic growth phase: Human bladder cancer cells T24 (purchased from the Cell Bank of the Chinese Academy of Sciences) were seeded in a 96-well plate at a concentration of 10,000 cells per well.

[0031] After overnight attachment, 0.1, 0.5, 1, 5 and 10 ug / ml ethanol extracts of jute seeds were administered, and 5, 10, 50 and 100 ug / ml mitomycin were used as positive controls. For each treatment, 6 replicate wells were set up, and the experiment was repeated 3 times.

[0032] After 24 hours of administration, the proliferation and growth rate of human bladder cancer T24 cells was detected by MTT method, and the IC50 value was calculated.

[0033] Such as figure 1 Shown, after acting on T24 cell 24h, the IC50 (growth semi-inhibition) dose of the rufous jute seed extract is 2.44ug / ml, which is only 16.6% of mitomycin 14.70u...

Embodiment 3

[0035] The preparation method of the fruit seed jute extract, adopts the following steps:

[0036] (1) Pulverize the wild round fruit type jute seeds, add sherwood oil 5 times of its weight and extract and degrease at room temperature for 48h;

[0037] (2) After the degreasing is completed, utilize 5 times of weight, and the volume fraction is 90% ethanol aqueous solution at room temperature and airtightly soaks the round fruit kind jute seed 2h after degreasing, then is heated to reflux temperature in water bath and carries out reflux extraction 1 time, and extraction time is 3h , collect the obtained extract, which is the ethanol extract of jute seeds, and store at -20°C.

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Abstract

The invention relates to a method for preparing the extract of corchorus capsularis seeds and the application of the extract. According to the method, corchorus capsularis seeds are crushed, and the petroleum ether 5 times the weight of corchorus capsularis seeds is added at the room temperature for extracting and degreasing. After the degreasing process, corchorus capsularis seeds are subjected to reflux extraction for 1-3 times in the ethanol aqueous solution 5-10 times the weight thereof, and the reflux extraction process lasts for 1-3 hours each time. The obtained extract is collected as the ethanol extract of corchorus capsularis seeds and is stored at 20 DEG C. The extract can be used for inhibiting the cell proliferation of human bladder cancer cells. Compared with the prior art, the extract is more efficient in growth proliferation effect for the human bladder cancer cell line T24 and has less influence on normal bladder epithelial cells. Therefore, the extract has an important significance on the treatment of bladder cancers, the comprehensive utilization of corchorus capsularis seed resources in China, and the increment of incomes for corchorus capsularis growers.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method and application of a jute seed extract. Background technique [0002] Bladder cancer is the most common malignant tumor of the urinary system, and its incidence rate ranks ninth among malignant tumors in the world and ranks first among malignant tumors of the urinary system in my country. Although 70-80% of primary bladder cancers are non-invasive (also known as superficial), they are prone to recurrence after surgery, with a recurrence rate as high as 70%, and about 15% of patients develop invasive bladder cancer. At present, regular intravesical infusion of mitomycin (MMC) and other chemotherapeutic drugs after transurethral resection of bladder tumor (TUR-BT) is mainly used clinically to prevent the recurrence and progression of non-invasive bladder cancer, but the effective rate is low (only 30-50%) and problems such as large toxic and side...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00A61K131/00
Inventor 朱云国程舟李珊杨晓伶李德芳陈安国
Owner TONGJI UNIV
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