Human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof

A ribosomal protein, human technology, applied in the field of biomedicine, to achieve the effect of easy preparation, easy preparation, preparation process and low cost

Active Publication Date: 2015-12-09
侯万儒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report at home and abroad on the strong growth inhibitory effect o

Method used

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  • Human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof
  • Human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof
  • Human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Such as figure 1 Shown: Construction of cell model and verification of anticancer effect

[0019] 1. Cell model

[0020] 1.1 Materials and methods

[0021] 1.1.1 Materials

[0022] 1.1.1.1 Cell lines: normal human liver cells ChangLiver (Chang's liver cells), human esophageal cancer Eca-109 cells, human breast cancer Bcap-37 cells, the above cell lines are all commercialized biomaterials.

[0023] 1.1.1.2 Reagents: RPMI1640 powder (purchased from Gibco, USA), fetal bovine serum (purchased from Sijiqing Biological Products Company, China), double antibodies (penicillin, streptomycin, purchased from Gibco).

[0024] 1.1.1.3 Human ribosomal protein RPS6 subunit anticancer peptide was synthesized by Shanghai Qiangyao Biotechnology Co., Ltd. The amino acid sequence of the human ribosomal protein RPS6 subunit anticancer peptide (polypeptide) is: ISGGNDKQGFPM; the molecular weight is 1250.41; the PI is 5.84. The purity is 92.91%. (The synthesis number of the anticancer p...

Embodiment 2

[0034] Xenograft model of human esophageal cancer cell Eca-109 in nude mice

[0035] Example purpose:

[0036] In accordance with the requirements of "Guiding Principles of Pharmacodynamics of Antineoplastic Drugs" and "Guiding Principles of Nonclinical Research Technology of Cytotoxic Antineoplastic Drugs", the anticancer peptide of human ribosomal protein RPS6 subunit (synthesis number cz-420, experiment The number is polypeptide 420) at a concentration of 1 ug / kg, whether it has inhibitory effect on the growth of human esophageal cancer cell Eca-109 xenograft tumor in nude mice and the strength of the effect.

[0037] 2.1 Materials and methods

[0038] 2.1.1 Test samples:

[0039] Peptide 420 was synthesized by Shanghai Qiangyao Biotechnology Co., Ltd. The amino acid sequence of the RPS6 subunit anticancer peptide (polypeptide) is: ISGGNDKQGFPM; the molecular weight is 1250.41; the PI is 5.84. The purity is 92.91%. (The synthesis number of the RPS6 subunit anticancer p...

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Abstract

The invention relates to a human ribosomal protein RPS6 subunit anticancer peptide fragment and application thereof. The human ribosomal protein RPS6 subunit anticancer peptide fragment has an amino acid sequence of ISGGNDKQGFPM, a molecular weight of 1250.41 and a PI of 5.84. The invention discloses the application of the human ribosomal protein RPS6 subunit anticancer peptide fragment in preparation of anticancer medicine. The human ribosomal protein RPS6 subunit anticancer peptide fragment has nearly no influence on growth of normal cells, has a growth inhibition effect on cancer cells, and has an obvious growth inhibition action on esophagus cancer Eca-109 cells with a growth inhibition rate up to 69.55%; a tumor inhibition rate is 59.09 by a human esophagus cancer Eca-109 nude mouse heterograft tumor model. The human ribosomal protein RPS6 subunit anticancer peptide fragment has an excellent anticancer effect and anticancer prospect.

Description

technical field [0001] The invention relates to a peptide segment and its application, in particular to a human ribosomal protein RPS6 subunit anticancer peptide segment and its application, belonging to the technical field of biomedicine. Background technique [0002] Ribosomes are organelles that catalyze protein synthesis. Mammalian ribosomes are composed of 4 RNAs and about 80 different proteins. The names of ribosomal proteins are related to the size of the ribosomal subunits. The large subunit ribosomal proteins are named L1 to L44, and the small subunit The base ribosomal proteins are named S1 to S31. The RPS6 protein subunit is a component of the 40s ribosomal protein, encoded by the RPS6 gene. The protein subunit belongs to the ribosomal protein S6E family, which exists in the cytoplasm, and the RPS6 protein subunit is the main substrate of protein kinases in ribosomes, and its five C-terminal serine residues are phosphorylated by different protein kinases change...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61K38/17A61P35/00
CPCA61K38/10C07K7/08C07K14/47A61K38/00
Inventor 侯万儒侯怡铃丁祥
Owner 侯万儒
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