Addition method for enhancing algae removal effect of allelochemicals
An allelochemical and algae-killing technology, applied in the field of ecological algae control, can solve the problems of rising water nutrient levels, potential safety hazards, water quality deterioration, etc., and achieve the effect of controlling re-emergence, ensuring ecological safety, and avoiding recovery of growth.
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Embodiment 1
[0019] N-phenyl-1-naphthylamine was chosen as the allelochemical, and the experiment was carried out in a 250mL Erlenmeyer flask. Microcystis aeruginosa in the logarithmic growth phase was inoculated into 100mL BG11 culture medium, so that the number of initial algal cells was 1.0×10 6 cells / mL or so. The specific dosing steps of the multiple intermittent dosing group include:
[0020] A. At the beginning of the experiment, N-phenyl-1-naphthylamine was added to the culture medium of Microcystis aeruginosa for 5 consecutive days, and it was continuously added 9 times or 10 times or 11 times every day from 8 o'clock in the morning;
[0021] B. The dose of N-phenyl-1-naphthylamine added each time is 40mg or 50mg or 60mg per cubic meter of water;
[0022] C. The intermittent time of each dosing is controlled at 0.9 hours or 1 hour or 1.1 hours.
[0023] At the same time, a control group for one-time high-dose dosing was set up, and the same N-phenyl-1-naphthylamine as the total...
Embodiment 2
[0034] Nonanoic acid was selected as the allelochemical, and the experiment was carried out in a 250mL Erlenmeyer flask. Microcystis aeruginosa in the logarithmic growth phase was inoculated into 100mL BG11 culture medium, so that the initial algal cell number was 1.0×10 6 cells / mL or so. The specific dosing steps of the multiple intermittent dosing group include:
[0035] A. At the beginning of the experiment, nonanoic acid was added to the culture medium of Microcystis aeruginosa for 5 consecutive days, and it was added 9 times or 10 times or 11 times every day from 8 o'clock in the morning;
[0036] B. The dose of nonanoic acid added each time is 40mg or 50mg or 60mg per cubic meter of water;
[0037] C. The intermittent time of each dosing is controlled at 0.9 hours or 1 hour or 1.1 hours.
[0038] At the same time, set up a one-time high-dose dosing control group, and dosing the same amount of nonanoic acid as the multiple dosing at 8:00 am on the first day of the exper...
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