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Pseudomonas arsenicoxydans and application thereof

A technology of arsenic oxidizing Pseudomonas bacteria, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc.

Active Publication Date: 2019-05-07
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there are also studies on the use of Pseudomonas arsenicoxidans to treat arsenite, but there is no report on the use of Pseudomonas arsenicoxidans to treat nitrate in groundwater

Method used

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  • Pseudomonas arsenicoxydans and application thereof
  • Pseudomonas arsenicoxydans and application thereof
  • Pseudomonas arsenicoxydans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0017] Specific implementation mode 1: In this implementation mode, a strain of Pseudomonas arsenicoxidans is Pseudomonas arsenicoxydans Y24-2, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, with a preservation number of CGMCC No.16655 and a preservation date of October 2018. January 29.

[0018] The screening method for Pseudomonas arsenicoxydans Y24-2 in the present embodiment is as follows: 100ml of low-temperature groundwater (water temperature 4°C) polluted by nitrate (nitrate concentration 140mg / L), at 2~6°C, 150rpm / Enrichment at min for 12-20 days, during which NaNO was continuously supplemented 3 and C 2 h 5 OH, the OD of the bacterial solution 600 After the value reaches above 0.5, serially dilute and inoculate on solid medium, cultivate at 2-6°C for 5-7 days, and select a typical single colony for purification and culture. The finally obtained pure culture strains were subjected to ni...

specific Embodiment approach 2

[0021] Specific embodiment 2: The medium formula used in the screening process of Pseudomonas arsenicoxidans in this embodiment is: NaNO 3 0.1~0.5g / L, MnSO 4 0.01~0.05g / L, (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O 0.01~0.10g / L, CaCl 2 0.01~0.05g / L, C 2 h 5 OH 0.1~2.0mL / L, Na 2 HPO 4 0.3~0.9g / L, MgSO 4 ·7H 2 O 0.01-0.05g / L, NaCl 0.3-0.9g / L, pH 7.0-7.4. Others are the same as the first embodiment.

[0022] If it is a solid medium, add 1.8g / L agar to the above formula.

specific Embodiment approach 3

[0023] Specific Embodiment Three: In this embodiment, Pseudomonas arsenicoxydans Y24-2 is used to remove pollutants in water.

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Abstract

The present invention discloses a pseudomonas arsenicoxydans and an application thereof, and relates to the pseudomonas arsenicoxydans and the application thereof. The pseudomonas arsenicoxydans is pseudomonas arsenicoxydans Y24-2 and stored in China General Microbiological Culture Collection Center, a preservation number is CGMCC No.16655, and a preservation date is October 29, 2018. When the pseudomonas arsenicoxydans is in a low-temperature and oligotrophic condition and a nitrate concentration in water is 20-100 mg / L, the pseudomonas arsenicoxydans can efficiently remove nitrite in groundwater and has a removal rate of the nitrate of 12.94-93.96 mg / L / h. The pseudomonas arsenicoxydans is applied to the technical field of environmental microorganisms.

Description

technical field [0001] The invention relates to a strain of Pseudomonas arsenooxidans and its application. Background technique [0002] Due to the application of agricultural chemical fertilizers, nitrate enters the groundwater environment through soil infiltration and affects the quality of groundwater. Because agricultural non-point source pollution is relatively difficult to control, the phenomenon of excessive nitric acid in groundwater in my country is relatively common. If drinking water contains excessive nitrate, it is easy to cause methemoglobinemia, and even induce cancer, which is harmful to the human body. In my country, especially in the northern region, about 60% of people use groundwater as their water source, and effectively removing nitrates in groundwater is crucial for ensuring the safety of water supply for residents. my country's drinking water hygiene standard (GB5749-2006) implemented since July 2007 also limits the maximum allowable concentration o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16C02F103/06
Inventor 张多英白也张彦龙赵丹丹曾伟民雷虹宫海楠
Owner HEILONGJIANG UNIV
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