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A kind of kitchen waste composite microbial degrading bacteria agent and its preparation method and application

A technology of composite microorganisms and kitchen waste, applied in the biological field, can solve the problems of large volume, low nutrient content, long fermentation cycle, etc., and achieve the effect of high microbial activity, low dosage and fast degradation rate

Active Publication Date: 2020-10-13
SHANGHAI NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation cycle is long, the nutrient content is low, and the volume after treatment is large, so it is only suitable for rural areas with inconvenient transportation

Method used

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  • A kind of kitchen waste composite microbial degrading bacteria agent and its preparation method and application
  • A kind of kitchen waste composite microbial degrading bacteria agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation method of the kitchen waste composite microbial degrading bacterial agent in the present embodiment specifically includes the following steps:

[0060] 1. Preparation of Compound Microbial Liquid

[0061] (1) The medium for strain culture of Bacillus amyloliquefaciens, Citrobacter freundii, Methylobacter radioresistant and Pantoea disperse, the solvent is water, the solutes and concentrations are as follows: beef extract 8g / L, peptone 5g / L , sodium chloride 5g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 5g / L, magnesium sulfate heptahydrate 1g / L, ammonium sulfate 1g / L, pH 7.2.

[0062] The culture conditions were as follows: temperature 28°C, rotation speed 200r / min, and culture time 20h.

[0063] The medium for the strain culture of Pseudomonas oryzae, the solvent is water, the solute and the concentration are as follows: potato 200g / L, glucose 15g / L, pH 7.5. The method for boiling the potato juice is as follows: take 200 g o...

Embodiment 2

[0082] The kitchen waste composite microbial degrading bacteria agent in this embodiment is a mixed strain composed of Bacillus amyloliquefaciens, Methylobacillus radioresistance, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii, and Staphylococcus korea It is mixed with a carrier, wherein the mixed bacterial species accounts for 15% of the total mass of the degrading bacterial agent, the mass percentage of water in the degrading bacterial agent is 20%, the pH of the degrading bacterial agent is 5.5, and each gram of the degrading bacterial agent contains The number of viable bacteria is 10 6 indivual.

[0083] Among them, the mass ratio of Bacillus amyloliquefaciens, Methylobacter radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii and Staphylococcus korii was 1.5:1:1:1:1:1.

[0084] The Bacillus amyloliquefaciens used in this example was preserved in Luojia Mountain (No. 299, Bayi Road), Wuchang District, Wuhan City, Hubei Province, China Colle...

Embodiment 3

[0109] The kitchen waste composite microbial degrading bacteria agent in this embodiment is a mixed strain composed of Bacillus amyloliquefaciens, Methylobacillus radioresistance, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii, and Staphylococcus korea It is mixed with a carrier, wherein the mixed bacterial species accounts for 25% of the total mass of the degrading bacterial agent, the mass percentage of water in the degrading bacterial agent is 10%, the pH of the degrading bacterial agent is 8.5, and each gram of the degrading bacterial agent contains The number of viable bacteria is 10 9 indivual.

[0110] Among them, the mass ratio of Bacillus amyloliquefaciens, Methylobacter radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii, and Staphylococcus korii was 3:1.5:1.5:1.5:1.5:1.

[0111] Bacillus amyloliquefaciens used in this example is preserved in Luojia Mountain (No. 299, Bayi Road), Wuchang District, Wuhan City, Hubei Province, China Col...

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Abstract

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kitchen waste composite microbial degrading bacterial agent and a preparation method and application thereof. Background technique [0002] In recent years, my country is in a period of rapid urbanization development, and the urban population is growing at a relatively high rate every year. With the gathering of population, the emission of urban waste is increasing, and the proportion of kitchen waste is increasing year by year. Because kitchen waste contains a large amount of macromolecular organic substances such as starch, cellulose, protein, oil, and water, it provides a good living environment for various microorganisms, which in turn leads to the proliferation of germs, mosquitoes and other harmful organisms. This is extremely harmful to human health and the urban environment. Although kitchen waste has high utilization value, its high moisture content and low calorific value...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/02B09B3/00C12R1/07C12R1/01C12R1/38C12R1/44
CPCB09B3/00C12N1/20C12N11/02
Inventor 李颂硕肖明张瑜张妍
Owner SHANGHAI NORMAL UNIVERSITY
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