Nucleic acid reagent, kit and system for detecting lower respiratory tract infection bacteria

Abstract

The disclosure relates to a nucleic acid reagent for detecting lower respiratory tract infection bacteria. The nucleic acid reagent comprises primers shown in SEQ ID NO.1-24 and probes shown in SEQ IDNO.27-38 which are stored in a respective and independent manner or in a mutual and arbitrary mixing manner. Through the primers and probes described above, the nucleic acid reagent, a kit, a systemand a method for detecting 12 types of lower respiratory tract infection bacteria such as streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, pseudomonas aeruginosa, klebsiella pneumonia, acinetobacter baumannii, enterobacter cloacae, burkholderia cepacia, escherichia coli, staphylococcus aureus, enterococcus faecium, and enterococcus faecalis are established, at the same time, internal reference genes are added as controls, the above 12 types of bacteria can be quantitatively detected through a double standard curve method, the uniform quantification treatment of samplescan be performed, the fast, comprehensive, sensitive, specific and automatic detection result determination is realized, and sensitivity, specificity, and simplicity of simultaneously detecting the above detection target genomes are significantly improved.

Description

technical field [0001] The present disclosure relates to the field of biotechnology, in particular to a nucleic acid reagent, kit and system for detecting lower respiratory tract infection bacteria. Background technique [0002] Community Acquired Pneumonia (CAP) refers to infectious lung parenchyma (including alveolar wall, that is, lung interstitium in a broad sense) inflammation caused by bacteria, viruses, chlamydia, mycoplasma and other microorganisms outside the hospital, including Pneumonia that develops during the incubation period after admission to the hospital due to pathogenic infection with a definite incubation period. The composition and drug resistance of CAP pathogens are significantly different in different countries and regions, and have changed over time. Hospital acquired pneumonia (HAP), also known as nosocomical pneumonia (NP), refers to a patient who does not exist at the time of admission and is not in the incubation period of infection, but occurs ...

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Problems solved by technology

[0003] The isolation and culture method is a common method for detecting bacteria in lower respiratory tract infections, and its test results are often used as the "gold standard" for the diagnosis of respiratory tract bacterial infections. However, the specificity and sensitivity of this method are poor, and it is not suitable for mild infections. Early diagnosis, unable to meet the needs of rapid detection

[0004] Serological testing is also a common method for detecting bacteria in lower respiratory tract infections. This method uses the relevant antigens produced by the human body as the detection target. However, the production of antigens is greatly affected by the course of the disease. It can only be detected after a week, which cannot meet the needs of rapid detection; moreover, for infants and young children, because the immune function is not yet fully developed, the ability to produce antibodies is poor, and there is a great possibility of false negatives in serological testing. The result is not accurate enough

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