288 results about "Enterococcus bacteria" patented technology

Enterococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerant Enterococcus are also provided.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention discloses an enterococcus faecium EF08 as well as a feed additive and a feed containing the enterococcus faecium EF08. The enterococcus faecium EF08 is collected with the number of CGMCC (China General Microbiological Culture Collection Center) No.5549 in CGMCC. The feed additive disclosed by the invention can be used for reducing the feed excessively consumed by harmful bacteria in intestines by virtue of an equalizing effect of bacteria for equalizing the intestinal environment, furthermore, the conversion rate of the feed for economic animals is increased, the feed for the economic animals can be effectively converted into a primary animal product with an economic value, and then, the effect of increasing the economic values of the economic animals can be improved.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention relates to preparation and application of a diarrhoea-resistant type special composite microecological preparation for piggies, belongs to the field of a feed additive, solves the technical problems of the special composite microecological preparation prepared from various beneficial microorganisms and having favorable biology diarrhoea-resistant effects, for the piggies, the product strain compatibility and the feeding effect stability, and relates to the diarrhoea-resistant type special composite microecological preparation for piggies. The diarrhoea-resistant type special composite microecological preparation is characterized by being formed by combining bacterium powder I, with bacterium powder II and a carrier, wherein the bacterium powder I is coated composite lactic acid bacteria consisting of coated enterococcus faecalis and pediococcus acidilactici; the bacterium powder II is composite bacillus formed by mixed fermentation of three probiotics of bacillus subtilis, bacillus coagulans and clostridium butyricum. Various beneficial microorganisms in the diarrhoea-resistant type special composite microecological preparation for piggies have the characteristics of being high in bacterium restraining capacity, rapid in propagation, capable of realizing synergistic symbiosis, rich in produced enzymes and high in enzyme activity. A technique of united fermentation of various bacterium strains and strain compatibility is adopted for preparing the diarrhoea-resistant type special composite microecological preparation, so that the diarrhoea-resistant type special composite microecological preparation is worth of wide popularization and application.

The invention aims to provide a production method for a compound microbial preparation for aquatic products. The production method comprises the following steps: respectively carrying out high-density liquid fermentation on strains of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium to obtain ultra-concentrated bacterium powder; and then preparing according to a ratio to obtain the compound microbial preparation. The production process is simple in process, is applicable to most existing fermentation processes and equipment and is good for being popularized and applied in a large scale.

The invention relates to the technical field of medical detection, and in particular, relates to a primer and probe combination and a kit for detecting human pathogenic bacteria. The kit comprises theprimer and probe combination for detecting pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, staphylococcus epidermidis, staphylococcus aureus, enterococcus faecium, human staphylococcus and acinetobacter baumannii. According to the kit, the primer and probe combination is adopted, multiple digital PCR is combined, the pathogenic bacteria types and the target nucleic acid copy number in the detection range can be rapidly and accurately identified, the detection method has high sensitivity, and positive detection of low-copy nucleic acid fragments of pathogenic bacteria is guaranteed; meanwhile, the variety of the pathogenic bacteria or the change of the copy number of the target nucleic acid fragment in the body of a pathogenic bacteria infected patient can be dynamically monitored, the treatment effect is assisted and evaluated in time, and reference is provided for optimization of a doctor clinical scheme.

The invention relates to the field of feed additives and particularly relates to a multi-strain composite microorganism feed additive and a preparation method thereof. The multi-strain composite microorganism feed additive comprises 26%-32% of bacillus, 15%-18% of enterococcus faecalis, 0.2%-0.3% of saccharomyces cerevisiae, 10.8%-17.2% of prebiotics, 4.7%-8.5% of sulfate, 0.2%-0.4% of potassium iodide, 0.2%-0.3% of sodium selenite and 32%-35% of mountain flour. The raw materials are sequentially subjected to liquid deep-layer fermentation and fixed sealed fermentation to form the multi-strain composite microorganism feed additive. The multi-strain composite microorganism feed additive provided by the invention can be used for reducing harmful bacterial colonies in intestinal tracts, and effectively expressing the effects of conditioning and protecting the health, increasing the feed utilization rate, preventing diseases and promoting growth, reducing the utilization of medicines including antibiotics and the like, improving the quality of animal products, reducing the generation of rotted substances, relieving foul smell if excrements and improving the breeding environment.

The invention relates to the field of microbial preparations and particularly discloses a compound microorganism bacterium agent and a preparation method and application thereof. The compound microorganism bacterium agent comprises lactic acid enterococcus faecalis, bacillus subtilis and bacillus megaterium, wherein the total viable count of the agent is not less than 3.5*108 cfu/mL. The compound microorganism bacterium agent is composed of reasonable strains according to composition characteristics of kitchen filtrate, can quickly degrade harmful substances in the kitchen filtrate, has a remarkable effect on degrading COD, BOD, ammonia nitrogen and the like in the kitchen filtrate and can effectively degrade organic matter to greatly relieve odor of the kitchen filtrate; the processed kitchen filtrate can also be used as a raw material of compound microorganism water-soluble fertilizer, so that the utilization rate of energy sources is increased, and environmental pollution caused by direct discharge of the kitchen filtrate is reduced.

The invention discloses enterococcus faecium, a culture medium thereof for high-density solid-state fermentation, and a high-density solid-state fermentation method, wherein the preservation number of the bacterial strain is CGMCC No.11155. Enterococcus faecium has the excellent performances of inhibiting bacteria, resisting acid and cholate, and the like, is tolerant to low moisture, and has relatively long shelf life under solid state fermentation condition. The culture medium contains wheat bran, bean pulp, cane molasses, activated carbon, calcium carbonate and beta- cyclodextrine. In the preparation technology, lactobacillus is inoculated into the culture medium for fermentation, and then drying is carried out at 45-65 DEG C, and thus obtaining a fermentation product. Compared with the like enterococcus faecium strains, the strain and the fermentation product provided by the invention have the advantages of high concentration of viable organisms, long shelf life and the like. The solid-state fermentation product of the strain provided by the invention can significantly improve the daily gain, the survival rate and the feed conversion ratio of nursery pigs, is obvious in economic benefit, and can be taken as a feed additive.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention provides a compound microorganism preparation. The compound microorganism preparation is composed of Bacillus cereus, bacillus licheniformis, halophilic pseudomonades, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium. The viable count of the compound microorganism preparation is as high as 8*1011CFU/g, and the compound microorganism preparation can be widely applied to the aquaculture water areas of fresh water and seawater. Ammonia nitrogen and nitrite in the water can be effectively degraded and water blooms can be restrained under the synergistic effect of the bacterial strains, therefore, the aquatic water can be purified, growing environment for breeding animals can be improved, and productivity can be improved.

The invention relates to a feed additive, particularly a phagostimulant fermented feed using bean pulp and bran as protein raw materials and a preparation method thereof. The phagostimulant fermented feed using bean pulp and bran as protein raw materials is prepared by fermenting the following components in percentage by weight: 8-15% of bean pulp, 55-70% of bran, 1.0-2.0% of Bacillus subtilis, 1.0-2.0% of Lactobacillus acidophilus, 1.0-2.0% of Enterococcus faecalis, 1.0-2.0% of microzyme, 0.1-0.5% of brown sugar, 0.02-0.08% of yeast extract, 0.02-0.08% of potassium dihydrogen phosphate, 0.02-0.08% of neutral proteinase, 0.02-0.08% of acid protease and 15-30% of water. The invention has the advantages of environmental protection, healthiness, favorable phagostimulant effect, strong capacity for killing pathogenic bacteria, and favorable therapeutic effect for porcine anorexia.

The invention provides a method for producing a deodorant microbial agent. The method comprises the following steps: carrying out high-density fermentation on streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, and then freezing and drying; adding a sodium citrate solution of which the concentration is 8-10% according to 1-5% of mass-volume ratio after mixing according to the ratio, so as to prepare a liquid deodorant product. The concentration of bad smells such as an ammonia gas, hydrogen sulfide and the like in the air in the places such as a toilet, a farm and a wasteyard can be effectively reduced; an odor-generating element is also restrained, so that the air quality and surroundings of people are significantly improved. The production method disclosed by the invention is simple, and adapts to most of existing fermentation technologies and equipments; large-scale popularization and application are facilitated.

The invention provides isolated polypeptide and nucleic acid sequences derived from Enterococcus faecalis that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

The invention aims at providing a compound microecological preparation for livestock and poultry excrement treatment and a preparation method and application thereof. For 100 g of livestock and poultry excrement, the compound microecological preparation needed to be added is prepared from, by volume, lactobacillus casei 0.03-0.10 Ml, enterococcus faecalis 0.03-0.10 mL, candida mycoderma bacteria 0.05-0.50 mL and bacillus subtilis 0.03-0.10 mL, wherein effective living bacterium number of bacterium liquid of the lactobacillus casei, the enterococcus faecalis, the candida mycoderma bacteria and the bacillus subtilis is 1.0 * 109 cfu/mL. The compound microecological preparation is utilized for fermentation of livestock and poultry excrement, the pH and the enterococcus faecalis number of the preparation can be reduced (P> 0.05), the content of indole is reduced by 58% (P< 0.05), and the compound microecological preparation has the great significance on fecal odor elimination.

The invention provides a composite microbial feed additive for chicken, which is characterized by comprising probiotics and prebiotics, wherein the probiotics comprise bifidobacterium, lactobacillus acidophilus, enterococcus faecium and bacillus licheniformis; and the prebiotics comprise xylooligosaccharides. The invention also provides a preparation method of the composite microbial feed additive for the chicken. The composite microbial feed additive has the advantages of reducing the morbidity of the chicken effectively, improving daily gain and feed-weight ratio effectively, reducing the production cost, reducing the usage amount of antibiotics effectively and improving the quality of animal byproducts.

The invention relates to a high-density fermentation medium and a fermentation technology for forage-use enterococcus faecium. By adopting the high-density fermentation medium and an in-batch fed-batch fermentation technology provided by the invention, the highest viable count of enterococcus faecium WEI-10CGMCC No.7746 under a 50L fermentation tank scale condition can reach 2.5X10<10> CFU/mL, and the highest viable count of enterococcus faecium WEI-10CGMCC No.7746 under a 5-ton fermentation tank scale condition can reach 2.9X10<10> CFU/mL, which are respectively 34 times and 36 times of those of in-batch fermentation of the traditional MES medium under the same fermentation scale condition, while the cost of the high-density fermentation medium is obviously reduced compared with that of the MRS medium, and thus the low-cost and high-efficiency production of enterococcus faecium as the microecological preparation is realized.

The invention discloses a medicinal cefetamet pivoxil hydrochloride composition for treating sensitive bacteria infectious diseases, and belongs to the field of medical technology. The medicinal cefetamet pivoxil hydrochloride composition is prepared from cefetamet pivoxil hydrochloride, compressible starch, microcrystalline cellulose 102, carboxymethyl starch sodium, hydroxypropylcellulose, glyceryl behenate and magnesium stearate. Experiments discover that compared with the prior art, the tablet prepared by novel crystal type compound of the cefetamet pivoxil hydrochloride is relatively low in high-molecular polymer content and good in stability; the increasing of the content of the high-molecular polymer is quite few with the increasing of storage time; meanwhile, the composition has more remarkable antimicrobial activity on pneumococcus and hemophilus influenzae as well as has relatively strong antimicrobial activity on enterococcus and staphylococcus.

The invention relates to a preparation method of a microencapsulated Enterococcus faecium live bacterium preparation and application of the preparation in breeding. The method employs a technological process of ''fermentation-coating-fermentation''. By adding 2g/L calcium chloride to a fermentation medium and conducting drying at 45DEG C, a microencapsulated Enterococcus faecium solid product with good sphericity and uniform size can be obtained, and its viable count can reach 11.57lgCFU/g. Compared with free Enterococcus faecium solid products, the microencapsulated Enterococcus faecium live bacterium preparation has stronger ability to tolerate a high temperature of 80DEG C and a simulated gastrointestinal fluid. After storage for 2 months under a normal temperature condition, the viable count basically has no change. The microencapsulated Enterococcus faecium live bacterium preparation has a simple pre-fermentation coating preparation process, good product appearance, strong stress resistance, high stability, and high encapsulation efficiency. Being use as a high activity microecological agent for feeding in actual production, the microencapsulated Enterococcus faecium live bacterium preparation can inhibit propagation of intestinal spoilage bacteria and pathogenic bacteria and intestinal infection, reduce the diarrhea rate, promote digestive absorption of nutrients, enhance immunity, improve feed intake and feed conversion rate, as well as lower feed-meat ratio.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

The invention provides a microbial preparation for purifying air. By utilizing the synergic effects of streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, the microbial preparation is capable of effectively reducing concentration of stink comprising ammonia gas, hydrogen sulfide and the like in the air at toilets, culturing farms, dumps and the like, and inhibiting stink-producing elements, so that air quality and human living environment can be substantially improved. A production method provided by the invention is simple, is applicable to majority of conventional fermentation technologies and equipment, and is suitable for large-scale popularization and application.

The invention discloses a medicine for treating gastrointestinal diseases by utilizing biological live bacteria, which is prepared from the following substances: 1 to 6 weight portions of oligoisomaltose, 1 to 6 weight portions of glucose, as well as 1 to 10 hundred million CFU/gram of bifidobacteria, 1 to 10 hundred million CFU/gram of lactobacilli and 1 to 10 hundred million CFU/gram of probiotics which take the total weight of the oligoisomaltose and the glucose as a radix number, wherein the probiotics are at least one of bacillus, lactococcus and enterococcus. The medicine is based on a modern microecology theory, adopts a probiotic live bacteria preparation to treat diseases such as gastritis, gastric ulcer, enteritis, diarrhea, constipation, indigestion, abdominal distension, diarrhea, intestinal cancer, acute and chronic hepatitis, low immunity and the like, and has the advantages of reliable action, broad function, safe use, no any toxic side effect and irritation, convenient administration and carrying and the like.