The invention relates to a flow cytometry detection method for tetracycline resistance bacteria in drinking water. The method comprises the following steps of (1) adding a water sample to be detected into a brown test tube, adding tetracycline mother liquor, enabling the concentration of tetracycline in the water sample to be detected to be equal to an MIC value thereof, putting the water sample to be detected into a culture box, and culturing for 24h at 27 DEG C; (2) transferring the cultured water sample into a flow cytometry sample injection pipe, adding an SYBR Green I coloring agent and a propidium iodide coloring agent for coloring, and after fully vibrating, culturing in dark at 37 DEG C for 10 to 15min; (3) transferring the cultured flow cytometry sample injection pipe into a flow cytometry, starting to inject a sample, determining the number of the tetracycline resistance bacteria, and dividing by a sample injection volume so as to obtain the concentration of the tetracycline resistance bacteria in the drinking water. Compared with the prior art, the number of the tetracycline resistance bacteria is detected by utilizing the flow cytometry, so that the flow cytometry detection method has the advantages of quickness, accuracy and quantitation, overcomes the defect that the nonculturable resistance bacteria cannot be detected through a traditional culture method, and is more reliable in result.