Common cephalanoplos herb trichloromethane extract and application thereof

A technology of chloroform and chloroform of thistle, applied in the field of chloroform extract of thistle, can solve the problems of large side effects, loss of effect, and easy generation of drug resistance.

Inactive Publication Date: 2017-09-08
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For decades, nearly 20 kinds of drugs have been developed for tuberculosis in the world, but they all have the disadvantages of large side effects and easy drug resistance, especially in recent years, drug-resistant and multidrug-resistant strains (Multiple drugs resistan

Method used

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  • Common cephalanoplos herb trichloromethane extract and application thereof
  • Common cephalanoplos herb trichloromethane extract and application thereof
  • Common cephalanoplos herb trichloromethane extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 The preparation method of Chloroform extract of thistle thistle of the present invention

[0024] Get 500g of thistle, pulverize with a pulverizer to make a coarse powder, and the coarse powder is passed through a Pharmacopoeia No. 3 sieve. Then the small thistle coarse powder was soaked in 95% ethanol for 24h, and the soaked coarse powder was extracted twice with an ultrasonic extractor, the ultrasonic power was 400W, 30min each time, the extracts were combined and filtered, and the filtrate was reduced by a rotary evaporator at 60°C. Concentrate under pressure to form an extract, put it in an oven and dry it to obtain a crude extract, dry it in vacuum to obtain a freeze-dried powder, and weigh it to obtain the extraction rate.

[0025] Accurately weigh 100g of ethanol extract freeze-dried powder of thistle, disperse in 500ml of distilled water respectively, add petroleum ether according to the ratio of ethanol extract aqueous solution and petroleum ether ...

Embodiment 2

[0029] Embodiment 2 Preparation of Chloroform Extract of Chrysanthemum thistle of the present invention

[0030] Accurately weigh 100g of thistle medicinal material coarse powder, put it into a 100ml round bottom flask, first add solvent petroleum ether to make it immerse in the medicine surface 1cm to 2cm high, ultrasonically extract for 30min, extract twice, collect petroleum ether extract, and extract Use a rotary evaporator to concentrate under reduced pressure at about 50°C to form an extract, use an oil bath to fully evaporate the residual organic solvent in the prepared extract, then vacuum dry the extract to obtain the extract, weigh , and calculate the extraction rate. After the petroleum ether part is extracted, respectively follow the order of chloroform, ethyl acetate, n-butanol and water to ultrasonically extract the extracts from the traditional Chinese medicine solvent parts of the corresponding solvents to obtain the petroleum ether, chloroform, and Ethyl acet...

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Abstract

The invention provides a common cephalanoplos herb trichloromethane extract. The common cephalanoplos herb trichloromethane extract is obtained by adding petroleum ether into common cephalanoplos herb crude powder or ethanol extract for extracting, then sequentially extracting extracts at corresponding solvent parts with trichloromethane, ethyl acetate, n-butyl alcohol and water, and collecting the extract at a trichloromethane part, so that the common cephalanoplos herb trichloromethane extract is obtained. The invention also provides application of the common cephalanoplos herb trichloromethane extract. The common cephalanoplos herb trichloromethane extract prepared by extracting by virtue of two systematic solvent processes has the minimum bactericidal concentration of 0.63mg/ml; and the experimental determination shows that the common cephalanoplos herb trichloromethane extract has the MIC value of 100mu g/ml and the MBC value of 400mu g/ml, and has better inhibiting and killing effects on a mycobacterium tuberculosis standard strain H37RV.

Description

technical field [0001] The invention relates to a chloroform extract of thistle, belonging to the field of medicines. Background technique [0002] Tuberculosis is a chronic, wasting infectious disease caused by Mycobacterium tuberculosis. Together with AIDS and malaria, tuberculosis remains a major threat to human health, especially in developing countries. According to the report of the World Health Organization, about 1 / 3 of the world's population has been infected with Mycobacterium tuberculosis, among which there are about 20 million tuberculosis patients, 8.7 million new tuberculosis patients are diagnosed every year, and about 3 million people die from the disease every year, surpassing AIDS and malaria , diarrhea and other infectious diseases, the death rate is the highest among diseases caused by single bacterial pathogen infection in the world, and tuberculosis has become one of the main causes of death from infectious diseases in adults all over the world. [00...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P31/06
CPCA61K36/28A61K2236/35
Inventor 严伟黎霞张宏李艾妮冉然
Owner SICHUAN NORMAL UNIVERSITY
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