Application of shepherd's purse in prevention and cure of tobacco ralstonia solanacearum

A technology of Ralstonia solanacearum and shepherd's purse, applied in the field of botanical pesticides, can solve the problems of few research reports

Active Publication Date: 2015-07-15
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many studies on the medical aspects of shepherd's purse, there are few research reports on the use of shepherd's purs

Method used

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  • Application of shepherd's purse in prevention and cure of tobacco ralstonia solanacearum
  • Application of shepherd's purse in prevention and cure of tobacco ralstonia solanacearum
  • Application of shepherd's purse in prevention and cure of tobacco ralstonia solanacearum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The inhibitory effect of the water extract of embodiment 1 shepherd's purse on tobacco Ralstonia solanacearum

[0020] Take shepherd's purse (whole plant, including roots, stems, leaves and seeds), wash it, dry it until the water content is 0.5-3.5%, and grind it into 100-120 mesh dry powder with a pulverizer at a speed of 9000r / min.

[0021] Weigh 20g of shepherd’s purse dry powder, add 400mL of sterile water, and shake at 120r / min for 24 hours at 25°C, filter, and collect the filtrate; repeat the extraction of the filter residue once, combine the filtrate twice, filter the filtrate through double-layer gauze and filter paper, and then pass through Filtrate with a 0.22 μm bacterial filter, place the obtained filtrate at a pressure of 0.09-0.1Mpa, a water bath temperature of 60°C, a condensation temperature of 6°C, and a rotation speed of 80r / min (rotary evaporation conditions, the same below), and rotate to a paste, Dilute to the water extract of shepherd's purse with ...

Embodiment 2

[0025] Example 2 Inhibitory effect of water extracts from different parts of shepherd's purse on tobacco Ralstonia solanacearum

[0026] Prepare shepherd’s purse root dry powder by the method in Example 1, weigh 20g of shepherd’s purse root dry powder, add 500mL of sterile water, shake at 80r / min at 26°C for 48h, and collect the filtrate; Filter through double-layer gauze and filter paper, and then filter through a 0.22 μm bacterial filter. The obtained filtrate is rotary-evaporated in a rotary evaporator to obtain a paste, and the volume is adjusted to 20 mL with sterile water to obtain the water extract of amaranth root. The water extracts of leaves, stems and seeds of shepherd's purse were prepared respectively by the above method.

[0027] The antibacterial effect of water extracts from different parts of shepherd's purse on R. solanacearum was determined by zone of inhibition method. Pour the sterilized NA medium into the petri dish and wait for it to cool down. Pipette ...

Embodiment 3

[0033] Example 3 The water extract of different parts of shepherd's purse to the MIC value of tobacco Ralstonia solanacearum

[0034] The root, stem, and seed water extraction paste of shepherd's purse are settled respectively with sterile water (the test proves that the active matter of shepherd's purse leaf extracted by sterile water has no antibacterial effect, so it does not measure its MIC), and the concentration of making it is 240mg / mL of the mother liquor, the mother liquor was diluted into 5 different concentrations of shepherd's purse active substance by the double dilution method, and the dilutions of 240mg / mL, 200mg / mL, 160mg / mL, 80mg / mL, and 40mg / mL were obtained successively. The antibacterial effect of water extracts of roots, stems, and seeds of different concentrations was determined by the ring method, and sterile water was used as a control, and each concentration was repeated 3 times. The highest dilution of the extract that grew completely aseptic was the ...

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Abstract

The invention belongs to the technical field of botanical pesticide, and in particular provides an application of shepherd's purse in prevention and cure of tobacco ralstonia solanacearum. The invention explores the inhibiting effect of shepherd's purse extract to the tobacco ralstonia solanacearum and finds out that the shepherd's purse extract has the inhibiting effect to the tobacco ralstonia solanacearum, and the inhibition ratio is 27.2 percents, wherein the inhibition effect of root extract, stem extract and seed extract is the strongest, and the inhibition effect of leaf extract is zero. Further research result shows that the MIC value of the root extract, the stem extract and the seed extract to the tobacco ralstonia solanacearum are all 80 mg/mL, and scientific basis is provided for developing and utilizing natural active materials of the shepherd's purse and researching and developing new biological pesticide.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, and in particular relates to the application of shepherd's purse in inhibiting and preventing tobacco bacterial wilt. Background technique [0002] Shepherd's purse (Capsella bursa-pastoris), commonly known as herb-protecting grass and intestine-cleaning grass, belongs to the herbaceous plant of the genus Capsella bursa in the family Brassicaceae. Shepherd's purse is mainly distributed in subtropical and temperate regions, and it can be seen growing all over my country, especially in fields, roadsides, courtyards, etc. [0003] Shepherd's purse is rich in organic acids, ergocrine, fumaric acid, flavonoids, alkaloids, amino acids, polypeptides, tannins and other active ingredients, which have the functions of improving eyesight, strengthening muscles and bones, lowering blood pressure, anti-inflammatory, analgesic, antispasmodic, Anti-bacterial, anti-cancer, anti-arrhythmia, scavengi...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P1/00
Inventor 廖晓兰刘双清谢燕张亚
Owner HUNAN AGRICULTURAL UNIV
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