Application of housefly MSP protein in antibiosis field

A protein, housefly technology, applied in the direction of antibacterial drugs, antifungal agents, peptide/protein components, etc., can solve the problems of high cost of artificial peptides, limited application prospects, etc.

Active Publication Date: 2016-11-02
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high cost of artificially synthesized

Method used

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  • Application of housefly MSP protein in antibiosis field
  • Application of housefly MSP protein in antibiosis field
  • Application of housefly MSP protein in antibiosis field

Examples

Experimental program
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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the examples.

[0029] 1 Materials and methods

[0030] 1.1 Materials

[0031] 1.1.1 Experimental housefly

[0032] Musca domestica was reared by the Pathogen Biology Laboratory of Guizhou Medical University at a temperature of 28°C, a relative humidity of 65%, and a photoperiod of 10L:14D.

[0033] 1.1.2 Experimental animals

[0034] New Zealand white rabbits, male, weighing 2Kg, were provided by the Animal Breeding Center of Guizhou Medical University, license number: SYXK (Qian) 2012-0001.

[0035] 1.1.3 Experimental plasmids and strains

[0036] PMD19-T cloning vector, Trans1-T1Phage Resistant Chemically Competent Cell cloning competent cells and Transetta(DE3) Chemically Competent Cell expression competent cells were purchased from Beijing Quanshijin Biotechnology Co., Ltd., pET28a(+) vector was preserved by our laboratory . Staphylococcus aureus (ATCC25923), Escherichia coli ...

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Abstract

The invention relates to an application of a housefly MSP protein in the antibiosis field, and belongs to the biotechnical field. The housefly MSP protein has an amino acid sequence represented by SEQ ID NO:1. A bioinformatics analysis result shows that the ORF full length of MSP gene is 495bp, the gene encodes 164 amino acids, the theoretic molecular weight is 17.4kDa, and the isoelectric point is 6.09; and the whole polypeptide chain is hydrophilic. An in-vitro antibiosis test result shows that the MSP has an inhibition effect on Escherichia coli, Staphylococcus aureus and Candida albicans, the corresponding MIC values are 0.3mg/ml, 0.2mg/ml and 0.002mg/ml respectively, and the MSP protein has protruding antibiosis activity to the Candida albicans. The housefly MSP protein is hopeful to become a clinically novel antibiotic.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of the housefly MSP protein in antibacterial aspects. Background technique [0002] The immune system of insects and the immune system of higher animals have relatively similar physiological functions, including immune defense, immune stabilization and immune surveillance. Compared with most mammals, insects do not have an acquired immune system such as T / B lymphocytes and immunoglobulins, so they lack specific antigen-antibody responses. The innate immunity of insects, also known as innate immunity, can quickly recognize and respond to pathogens when the body is invaded by pathogens. The innate immune response is mainly involved in the recognition of pathogen-related molecular patterns by pattern recognition receptors on the cell membrane or cytoplasm, including cellular immunity and humoral immunity. Surrounding and phagocytosis of blood cells are called cellular ...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P31/04A61P31/10
CPCA61K38/00C07K14/43577Y02A50/30
Inventor 国果陶如玉修江帆杨蔚锦付萍吴建伟
Owner GUIZHOU MEDICAL UNIV
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