Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing sterol derivative through Burkholderia conversion and application

A technology of Burkholderia and Krkholderia, applied in the field of Burkholderia transformation and preparation of sterol derivatives

Inactive Publication Date: 2020-07-31
JIANGNAN UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The Burkholderia screened in our laboratory can not only produce cholesterol esterase and cholesterol oxidase, but also degrade cholesterol, sitosterol, stigmasterol and other sterol substances, and use Burkholderia to transform cholesterol into cholesterol -4-ene-3,6-dione has not been clearly reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing sterol derivative through Burkholderia conversion and application
  • Method for preparing sterol derivative through Burkholderia conversion and application
  • Method for preparing sterol derivative through Burkholderia conversion and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Example 1: α-cyclodextrin system B.cepacia converts cholesterol to prepare cholest-4-ene-3,6-dione

[0036] (1) Slant culture: Take Burkholderia ZWS15 stored in a glycerol tube at -40°C, inoculate 30 μL into LB solid medium, place it in a constant temperature incubator at 37°C, and cultivate for 24 hours until a single colony grows;

[0037] (2) Seed culture: Pick a single colony from the strain cultivated in step (1) under sterile conditions and inoculate it in 50 mL LB liquid medium, and culture it in a shaker at 37°C with a rotation speed of 200 rpm for 9-13 hours to OD 600 5.0 to 6.0.

[0038] (3) B. cepacia conversion of cholesterol: the seed solution cultivated in step (2) was inserted into the transformation medium at an inoculum size of 5% by volume, and cultured at 30° C. and 200 rpm for 48 hours. The group without cyclodextrin or its derivatives was used as the control.

[0039] (4) TLC separation and extraction of the conversion product of cholesterol: the ...

Embodiment 2

[0045] Example 2: β-cyclodextrin system B.cepacia converts cholesterol to prepare cholest-4-ene-3,6-dione

[0046] See embodiment 1 for the specific embodiment.

[0047] The amount of fixed cholesterol in the transformation medium was 2g / L, that is, 5.18mM; the molar ratio of β-cyclodextrin to cholesterol was 1:2, 1:1 and 2:1. The two were pretreated with 60W ultrasonic for 10-15min and then added to the transformation medium.

[0048] Analysis and detection: When the molar ratio of β-cyclodextrin and cholesterol is 1:2, 1:1 and 2:1, the molar conversion rate of cholest-4-ene-3,6-dione is 0.38% respectively , 0.36% and 0.42%. The conversion rate of the control group under the same conditions was 0.2%.

Embodiment 3

[0049] Example 3: γ-cyclodextrin system B.cepacia converts cholesterol to prepare cholest-4-ene-3,6-dione

[0050] See embodiment 1 for the specific embodiment.

[0051] The amount of fixed cholesterol in the transformation medium was 5.18mM; the molar ratio of γ-cyclodextrin to cholesterol was 1:2, 1:1 and 2:1. The two were pretreated with 60W ultrasonic for 10-15min and then added to the transformation medium.

[0052] Analysis and detection: when the molar ratio of γ-cyclodextrin to cholesterol is 1:2, 1:1 and 2:1, the molar conversion rate of cholest-4-ene-3,6-dione is 0.51% respectively , 0.35% and 0.26%. The conversion rate of the control group under the same conditions was 0.2%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a sterol derivative through Burkholderia conversion and application, and belongs to the technical field of sterol microbial conversion. Burkholderia is added into a reaction system containing cyclodextrin or a derivative thereof, so that the product conversion yield can be increased to 12-15% from 0.1-0.3% of a control group; and in addition, an organic solvent does not need to be added in the preparation process of the method, so that the method is more environment-friendly, economical and safe.

Description

technical field [0001] The invention relates to a method and application for preparing sterol derivatives by transformation of Burkholderia bacteria, and belongs to the technical field of sterol transformation by microorganisms. Background technique [0002] Cholesterol is an important sterol compound that can be used as a raw material for steroidal drugs. Some microorganisms can excise the saturated side chain of cholesterol and oxidize the specific part of the steroid nucleus, so as to obtain a variety of derivatives with medicinal value. Liu WH et al. used Mycobacterium sp. to convert cholesterol into testosterone (TS); Nagasawa et al. used Arthrobacter simplex to degrade cholesterol and successfully produced androsta-1,4-diene-3,17-dione (ADD); Guo Qian et al. used Rhodococcus sp. converts cholesterol to cholest-4-en-3-one. In addition to the above derivatives, cholesterol can also be converted by microorganisms into cholest-4-ene-3,6-dione. [0003] Cholester-4-ene-3...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P33/02C12R1/01
CPCC12P33/02
Inventor 张玲王武杨海麟武迪
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com