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Burkholderia sp. for aerobic degradation of benzpyrole, and application of burkholderia sp.

A technology of Burkholderia and indole, applied in the field of microorganisms, to achieve the effect of good temperature and pH tolerance and strong degradation ability

Inactive Publication Date: 2015-09-30
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the degradation of indole by this genus, and there is only one report on the degradation of indole with the assistance of gallic acid, an external carbon source.

Method used

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  • Burkholderia sp. for aerobic degradation of benzpyrole, and application of burkholderia sp.
  • Burkholderia sp. for aerobic degradation of benzpyrole, and application of burkholderia sp.
  • Burkholderia sp. for aerobic degradation of benzpyrole, and application of burkholderia sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening of Burkholderia strains

[0026] The activated sludge from the reactor was repeatedly diluted and plated on the inorganic salt solid medium plate, and cultured at 30°C. Finally, a well-growing single colony was picked and cultivated in a liquid inorganic salt medium, and the bacterial liquid was obtained at 30°C and 150 rpm.

Embodiment 2

[0027] Example 2: 16S rRNA molecular identification of strains

[0028] Pick a single colony grown on an inorganic salt solid medium plate and dissolve it in 10 μL of sterilized water, denature at 99°C and centrifuge to take the supernatant as a template for PCR amplification reaction. Use TaKaRa 16S rDNA Bacterial Identification PCR Kit with Forward primer / Reverse primer as primers to amplify the target fragment. The total volume of the PCR reaction system is 50 μL: PCR Premix 25 μL, Forward Primer 0.5 μL, Reverse Primer 0.5 μL, template DNA 5 μL, sterile ultrapure water 19 μL. The PCR reaction conditions were: pre-denaturation at 94°C for 5 min, after 30 cycles, including denaturation at 94°C for 1 min, annealing at 55°C for 1 min, extension at 72°C for 1.5 min, after 30 cycles, extension at 72°C for 5 min, Take 5 μL of PCR products for 1% agarose gel electrophoresis, and UV detection after EB staining. Use TaKaRa Agarose Gel DNA Purification Kit Ver. 2.0 (TaKaRa, Dalian...

Embodiment 3

[0031] Example 3: Determination of Substrate Broad Spectrum of Burkholderia IDO3

[0032] Utilize the bacterium liquid in embodiment 1, adopt the inorganic salt culture medium ((NH 4 ) 2 SO 4 2.0 g / L, KH 2 PO 4 2.0 g / L, Na 2 HPO 4 12H 2 O 3.28 g / L, FeCl 3 0.00025 g / L) culture, 5% inoculum, specifically investigated the growth of strain IDO3 with a variety of compounds as the sole carbon source, including benzoic acid, tryptophan, phenol, catechol, salicylic acid, indole, etc. The concentration of each compound is 50 mg / L.

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Abstract

Burkholderia sp. for aerobic degradation of benzpyrole and the application of burkholderia sp. belong to the technical field of microorganism. The strain separated from activated sludge of a reactor is preserved in the Center for General Microorganisms of China Committee for Culture Collection of Microorganisms (CCCCM) on January 28, 2015, with the preservation number of CGMCC No.,10457. 16S Rrna gene sequence determination shows that strain IDO3 and Burkholderia have high similarity, therefore, the strain is classified and named as Burkholderia sp. IDO3, and the accession number of the gen bank of the gene sequence of the strain 16S rRNA is KP895480. The strain has a relatively stronger degradation capacity to benzpyrole, also has excellent Ph and temperature tolerances, and shows the application prospect in treating waste water with high concentration benzpyrole.

Description

technical field [0001] The invention relates to a Burkholderia strain for aerobically degrading indole and application thereof, belonging to the technical field of microbes. technical background [0002] With the development of industry, the situation of water pollution in our country is becoming more and more severe. Coking wastewater, dye wastewater, pharmaceutical wastewater, pesticide wastewater and other industrial wastewater contain a large number of nitrogen heterocyclic compounds, such as pyrrole, indole, pyridine, quinoline, isoquinoline and their derivatives. Nitrogen heterocyclic compounds have a conjugated system, are not easily destroyed in the metabolic process, and have poor biodegradability, and most of these substances have carcinogenic, teratogenic, and mutagenic effects, posing serious threats to the environment and human health. Therefore, many researchers have carried out studies on the biodegradation characteristics of nitrogen-containing heterocyclic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01C02F101/38C02F103/10
CPCA62D3/02A62D2101/28C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 曲媛媛刘紫嫣马桥沈文丽厉舒祯由胜男裴晓芳张世明王经伟李端行张照婧李会杰
Owner DALIAN UNIV OF TECH
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