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Burkholderia lata strain PN1 and application thereof

A technology of Burkholderia latta and Holderia, which is applied in the fields of application, bacteria, and fungicides, can solve the problems of endophytic bacteria in moso bamboo that have not been reported to resist diseases and promote growth, and there are few endophytic bacteria in moso bamboo , to achieve the effects of excellent strain resources, strong secretion of IAA ability, and strong phosphorus solubilizing ability

Active Publication Date: 2020-05-05
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few related studies on the endophytic bacteria of moso bamboo, and the research on the disease resistance and growth promotion of endophytic bacteria in moso bamboo has not been reported yet.

Method used

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  • Burkholderia lata strain PN1 and application thereof
  • Burkholderia lata strain PN1 and application thereof
  • Burkholderia lata strain PN1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and identification of endophytic bacteria PN1 from Phyllostachys pubescens

[0026] The three- to four-year-old moso bamboos with good growth were selected from the moso bamboo plantations in Dagang Forest Farm, Guanshan Forest Farm and Jinggangshan Dajing Forest Farm in Yichun City, Jiangxi Province, respectively. The topsoil was removed, and the root system of Moso bamboo was dug out with a shovel to collect the fine roots of Moso bamboo. Rinse the root system of moso bamboo with distilled water for 3 times, and carry out surface disinfection according to the following steps: soak in 75% v / v alcohol for 1 min, soak in 3.25% v / v sodium hypochlorite for 3 min, soak in 75% v / v alcohol for 30 seconds, and then rinse with sterile water for 3 times . Grind the treated root tissue and collect the grinding liquid. Coat the NA plate with sterile water after the last rinse (the formula of NA medium: 3g of beef extract, 10g of peptone, 5g of NaCl, 16g of ag...

Embodiment 2

[0033] Embodiment 2: Bacterial strain PN1 antagonizes the pathogenic bacteria of Phyllostachys pubescens blight

[0034] Use a hole puncher (diameter=5mm) to get the bacteria cake at the edge of the colony of the pathogenic bacteria C.phyllostachydis (C.phyllostachydis) of Phyllostachys pubescens for 5 days, and connect it to the center of the PDA plate, and mark the bacterial strain to be tested activated with the NA slope The line was inoculated on both sides of the PDA plate, and only the pathogenic bacteria were inoculated as the control (CK), and each treatment was replicated three times. Incubate at 28°C for 4 days to observe the presence or absence of the bacteriostatic zone, and measure the width of the bacteriostatic zone.

[0035] Inoculate the Burkholderia latta PN1 with better bacteriostasis in the plate confrontation test on NB medium (the formula of NB medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH 7.2, after mixing evenly ready to steri...

Embodiment 3

[0039] Embodiment 3: Determination test of bacterial strain PN1 phosphorus solubilizing ability

[0040] Burkholderia latta PN1 was inoculated on NBRIP solid medium (glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, agar 15g, mix uniformly and sterilize to obtain), cultivate for 4 days and observe whether phosphorus-dissolving circles are produced. Quantitative determination of the phosphorus-solubilizing capacity of Burkholderia latta PN1 was performed using the method of Pikovskaya et al. After shaking and culturing Burkholderia latta PN1 in NB medium for 24 hours, inoculate it into a 100mL Erlenmeyer flask containing 50mL of NBRIP culture solution at an inoculation amount of 1% v / v, and connect the same volume of blank seed solution. NBRIP culture solution (without strains) was used as the control. Each inoculation treatment was repeated five times, 30°C, 180r / min shaking culture for 6 days, t...

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Abstract

The invention discloses a burkholderia lata strain PN1 and application thereof. The classification name of the strain is Burkholderia lata, the strain number is PN1, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2019880. The strain PN1 screened out from phyllostachys edulis root systems has a significant inhibiting effect on phyllostachys edulis shoot blight pathogens ceratosphaeria phyllostachydis, a bacteriostasis rate of fermentation broth of the strain PN1 screened out from the phyllostachys edulis root systems can reach58.82%, and the strain PN1 screened out from the phyllostachys edulis root systems has efficient capability of phosphate solubilizing and IAA secretion; and the phyllostachys edulis endophytic bacteria PN1 can significantly promote growth of phyllostachys edulis seedlings, and test data shows that a liquid bacterial agent produced from the strain PN1 significantly increases growth indexes of the ground diameter, the seedling height, chlorophyll and the like of phyllostachys edulis. Therefore, excellent strain resources are provided for development of an environment-friendly bio-fertilizer special for the phyllostachys edulis.

Description

technical field [0001] The invention relates to the technical field of microorganisms and biological fertilizers, in particular to Burkholderia latta PN1 and its application. Background technique [0002] Moso bamboo (Phyllostachys edulis) is the main economic forest species for agricultural and forestry industry structure adjustment, forestry efficiency enhancement and forest farmers' income increase in southern my country. Since the moso bamboo dieback disease was first discovered in Huangyan, Zhejiang, it has successively broken out in southern provinces, causing serious economic losses and severely restricting the cultivation of moso bamboo resources and the development of bamboo industry. The pathogenic bacterium of the disease is Ceratosphaeria phyllostachydis, which has brought great harm to the cultivation of bamboo forests and has been listed as the national forest plant quarantine object. In addition, with the increase in the demand for moso bamboo in recent years...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C05G3/40C12R1/01
CPCA01N63/00C05G3/00C12N1/205C12R2001/01
Inventor 毛瑢万松泽张扬石福习张芸陈伏生
Owner JIANGXI AGRICULTURAL UNIVERSITY
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