Strain for producing 2, 5-furandimethanol and application of the strain

A technology for furandimethanol and strains, which is applied in the biological field and can solve problems such as difficulty in meeting industrial production needs, low yield of 2,5-furandimethanol, low tolerance concentration and the like

Active Publication Date: 2021-04-30
NANJING POLYTECHNIC INSITUTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, because 5-hydroxymethylfurfural is an inhibitor of microorganisms and enzymes, the tolerance concentration of microorganisms to 5-hydroxymethylfurfural is low, so the yield of 2,5-furandimethanol is low, and it is difficult to Meet the needs of industrial production

Method used

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  • Strain for producing 2, 5-furandimethanol and application of the strain
  • Strain for producing 2, 5-furandimethanol and application of the strain
  • Strain for producing 2, 5-furandimethanol and application of the strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The 5-hydroxymethylfurfural-tolerant strain Burkholderia contaminans NJPI-15 was obtained by screening soil samples around Nanjing Dachang Chemical Plant with high concentration of 5-hydroxymethylfurfural as screening pressure.

[0030] (1) Add deionized water to the soil sample, mix well, then centrifuge, take the supernatant and apply it to the solid medium for screening for cultivation, the cultivation temperature is 35°C, and the cultivation time is 24-48h. This method can screen high-concentration 5-hydroxymethylfurfural-resistant microorganisms.

[0031] (2) Pick a single colony from the solid medium screened in step (1) into a liquid enrichment medium for enrichment culture at a culture temperature of 35° C., and obtain an enrichment culture medium after culturing for 12 to 14 hours.

[0032] (3) Inoculate the enrichment culture solution obtained in step (2) into the liquid enrichment fermentation medium according to the inoculation amount of 1% for cultivation. ...

Embodiment 2

[0038] This example illustrates the impact of different co-substrates on the yield of 2,5-furandimethanol and the conversion rate of 5-hydroxymethylfurfural

[0039] The onion Burkholder NJPI-15 was inoculated in the liquid enrichment medium and cultured at 35°C for 16h. The fermentation liquid is centrifuged, the bacterial cells are collected, the bacterial cells are washed twice with a pH 7.0 phosphate buffer solution, and the onion Burkhold NJPI-15 bacterial cells obtained after centrifugation are the biocatalyst.

[0040] Preparation of reaction solution: pH 7.0, 50 mM phosphate buffer solution containing 100 mM 5-hydroxymethylfurfural. According to the ratio of adding 20 mg wet weight thallus in every milliliter of reaction solution, add Burkhold onion NJPI-15 thallus respectively in each reaction solution, then add a kind of co-substrate (asparagus) that final concentration is 50mM respectively Amino acid, nicotinic acid, glutamine, glycine, ribose and xylose), after cu...

Embodiment 3

[0043] This example illustrates the impact of different temperatures on the yield of 2,5-furandimethanol and the conversion rate of 5-hydroxymethylfurfural

[0044] The onion Burkholder NJPI-15 was inoculated in the liquid enrichment fermentation medium, and cultured at 35°C for 16h. The fermentation liquid is centrifuged, the bacterial cells are collected, the bacterial cells are washed twice with a pH 7.0 phosphate buffer solution, and the onion Burkhold NJPI-15 bacterial cells obtained after centrifugation are the biocatalyst.

[0045] Preparation of reaction solution: pH 7.0, 50 mM phosphate buffer solution containing 100 mM 5-hydroxymethylfurfural and 70 mM glutamine. According to the ratio of adding 20 mg wet weight thallus in every milliliter of reaction solution, add onion Burkhold NJPI-15 thallus in reaction solution, respectively at different temperature (20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ °C, 45 °C), the shaker speed was 200rpm, and cultured for 6h, and samples were take...

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Abstract

The invention provides a strain for producing 2, 5-furandimethanol and application of the strain, and belongs to the technical field of biology. The invention relates to a strain for producing 2, 5-furandimethanol, which is classified and named as a Burkholderia cepacia NJPI15 strain, and the preservation number of the strain is CCTCC NO: M 2020636. A method for converting 5-hydroxymethylfurfural into 2, 5-furandimethanol is characterized in that the strain is used as a biocatalyst to convert 5-hydroxymethylfurfural into 2, 5-furandimethanol. The strain Burkholderia contaminans NJPI15 with high tolerance to 5hydroxymethylfurfural is separated from chemically polluted soil, and the yield of 2, 5-furandimethanol can be high. The strain Burkholderia contaminans NJPI15 disclosed by the invention has the advantages that the yield is high;.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacterial strain producing 2,5-furandimethanol and its application. Background technique [0002] Rational development and utilization of natural biomass resources is the main direction of social and economic sustainable development in the 21st century. 5-Hydroxymethylfurfural (abbreviated as HMF), as an important bio-based platform compound that can be converted from biomass resources, is one of the "Top 10+4" bio-based platform chemicals. There are two reactive functional groups in 5-hydroxymethylfurfural, the aromatic ring and the formyl group, which make the biomolecules highly chemically reactive. Studies have shown that HMF can be converted into a variety of valuable products, such as 5-hydroxymethyl-2-furancarboxylic acid (HMFCA), 2,5-diformylfuran (DFF), 2,5-furandimethanol (BHMF ) and 2,5-furandicarboxylic acid (FDCA). 2,5-furandimethanol is a multifunctiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/01
CPCC12P17/04
Inventor 李冰峰潘鑫常思源张媛周倩
Owner NANJING POLYTECHNIC INSITUTE
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