48results about How to "Low requirements for cultivation conditions" patented technology

The invention relates to EV71 virus-like particles and hand-foot-and-mouth disease vaccine prepared from the EV71 virus-like particles. The EV71 virus-like particles are prepared according to the following steps: (1) constructing recombinant plasmid: respectively connecting pGAPZ alpha A plasmid with P1 protein gene of intestinal EV71 virus and 3CD protease gene to construct P1-pGAPZ alpha A and 3CD-pGAPZ alpha A recombinant plasmid; (2) transferring the recombinant plasmid to expression bacterial strains: sequentially transferring the recombinant plasmid to Pichia pastoris SMD1168 expression bacterial strains to obtain P1-pGAPZ alpha A-3CD-pGAPZ alpha A-SMD1168 recombinant expression strains; and (3) culturing thallus and purifying the EV71 virus-like particles: culturing the Pichia pastoris recombinant expression bacterial strains, centrifugalizing to separate supernate, and carrying out sucrose density gradient centrifugation on precipitation of the supernate after ultracentrifugation, thus obtaining the EV71 virus-like particles. In the invention, the EV71 virus-like particles are easy to obtain through thallus culture, have good stability, are easy to purify and suitable for preparing vaccine, and are convenient for industrial production.

The invention discloses an application of green gram starch and green bean dregs in inducing fusariumgraminearum schw to massively generate conidium, and also discloses a corresponding bean dregs culture base or a green bean dregs culture base; the culture base is prepared from, by weight, 20-80 g of green gram starch or green bean dregs, 15-25 g of agar powder, and 1000 ml of distilled water. The invention further discloses a method for inducing fusariumgraminearum schw to massively generate conidium. The fusariumgraminearum schw has big conidium output on the green gram starch or green bean dregs base; the conidium output per cm2 is up to 107 pieces; moreover, massive large conidium piles can be generated; secondly, the method is simple and high low requirement on culture condition, and needs not the additional near ultraviolet lamp induction; besides, the culture base is simple in component, easy to obtain raw materials and low in cost.

The invention discloses a culture medium and a culture method for promoting fast propagation of microalgae. The culture medium for promoting the fast propagation of microalgae is characterized in that hydroxyl-containing three-carbon organic matters are added into the conventional culture medium, the concentration of the hydroxyl-containing three-carbon organic matters in the culture medium is not greater than 1g/L, preferably, the concentration of the hydroxyl-containing three-carbon organic matters is 0.05 g/L to 0.8 g/L, and the hydroxyl-containing three-carbon organic matters comprise glycerin, 1,3-propylene glycol, 1,2-propylene glycol, propyl alcohol, isopropanol, glyceric acid and glyceraldehydes. The culture method for promoting the fast propagation of the microalgae comprises the steps that: microalgae liquid and culture media of the hydroxyl-containing three-carbon organic matters are mixed in a bioreactor according to the volume proportion of 1:15-1:30, the biomass of initial microalgae in the bioreactor is 40 mg/L to 80mg/L, the culture temperature is 20 DEG C to 37 DEG C, the stirring speed is 100 rpm to 500 rpm, and the illumination intensity is 1000 lux to 5000 lux. The culture medium has the advantages that the preparation method is simple, the cost is low, and the culture medium is suitable for industrial application; and when the culture medium is adopted for microalgae culture, the complicated CO2 supply equipment and the illumination condition are not needed, and the autotrophic metabolic efficiency of the microalgae and the CO2 fixing efficiency can be fast improved, so the problem of slow accumulation of microalgae biomass is solved.

The invention discloses an entomopathogenic nematode symbiotic bacteria strain for inhibiting various plant fungal diseases and application. The bacteria strain is a high-toxicity bacteria strain NN6of xenorhabdus bovienii, the preservation number is CCTCC NO: M 2020097, and the bacteria strain is preserved in China Center for Type Culture Collection on April 29, 2020. The xenorhabdus bovienii NN6 disclosed by the invention has relatively strong ability of inhibiting growth of fusarium oxysporum f.sp.niveum, fusarium oxysporium f.sp.cucumerinum, fusarium graminearum, pyricularia grisea, botrytis cinerea and phytophthora capsici hypha, the ability is increased along with the increase of volume concentration, and the antibacterial effect is obvious. The bacteria strain also has the effect of inhibiting the germination of fusarium spores, and the inhibition effect is remarkable. The bacteria strain has the characteristics of low culture condition requirement, long lasting period, environment-friendly and non-toxic source and the like, has important significance in protecting the ecological environment and improving the added value of agricultural products, and has good market development and application prospects due to the advantage of wider antibacterial spectrum.

The invention discloses bacterium paenibacillus polymyma SHL-1 and an application thereof in preventing and controlling stalk rot of cymbidium sinense. The bacterium paenibacillus polymyma SHL-1 with a preservation number of CCTCCM2011441 is preserved in the China Center for Type Culture Collection on December 2, 2011. In the invention, the bacterium paenibacillus polymyma SHL-1 is inoculated to an LB (Langmuir-Blodgett) liquid culture medium with pH of 7.0, and is cultured for 48 hours in a oscillation way at 200r/min at a temperature of 28 DEG C, so that a biological preparation can be prepared. The biological preparation has a strong inhibition effect to the stalk rot of cymbidium sinense, and has environmentally-friendly non-toxic sources and small influence to biological environment;and furthermore, the biological preparation has low requirements to the culture condition, has an inhibition effect to various pathogenic fungi, and has good development and application prospects.

The invention provides a method for preparing plant monoploid idiosome and monoploid plant, belonging to plant cultivating field, the method for preparing plant monoploid idiosome comprising the following steps: a. shearing off 1-3 column cap in the flower before flower blooming; b. carrying out artificial pollination for the left column cap when flower blooming; c. drawing the monoploid idiosome from the unfertilized blastocyst when the fruit is becoming mature. The method for preparing monoploid plant comprises the following steps: a. inoculating the prepared monoploid idiosome into the culture medium for culturing sprout; b. training sprout, replanting for culturing. The invention is characterized by the simple operation, no need special instrument, low cost, high success rate, suitable for field application and bright practical applying prospect.

The invention belongs to the technical field of biological prevention and treatment of plant diseases, and discloses litchi endophytic burkholderia gladioli and an application of the litchi endogenousburkholderia gladioli in prevention and treatment of litchi anthracnose and litchi downy blight. The name of the bacterial strain is Burkholderia gladioli SZPT16, and is preserved in Guangdong Province Microbial Culture Collection Center, GDMCC for short, in Guangzhou Institute of Microbiology, the address is Institute of Microbiology, Guangdong Province, 59 # building, 100 # courtyard, Xianlie Middle Road, Guangzhou, the preservation number is GDMCC No: 61168, and the preservation time is August 25, 2020. The strain disclosed by the invention is derived from litchi endophytic bacteria, and the strain and a biological agent thereof both have a strong inhibition effect on litchi anthrax bacteria and peronophythora litchi bacteria, and have an excellent biocontrol effect; and the product isenvironment-friendly and non-toxic in source, is safer and more reliable to eat litchi and processed products thereof, and has good development and application prospects.

The invention discloses bacillus velezensis F3A and application of the bacillus velezensis F3A in biological prevention and control of Chinese wolfberry anthracnose, the preservation number of the bacillus velezensis F3A is CGMCC21518, and the bacillus velezensis F3A is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 23, 2020. The strain has a relatively good prevention and treatment effect on Colletotrichum acutatum, shows broad-spectrum antibacterial activity on pathogenic bacteria such as radix astragali root rot, eggplant sclerotinia rot, tomato leaf mold, tomato early blight and tomato gray mold, has relatively high activity of producing IAA (Indoleacetic Acid), protease and glucanase, and has the capacity of dissolving organic phosphorus and inorganic phosphorus. The new biopesticide and microbial agent developed by using the strain have good application prospects.

The invention provides a marine microbial flocculant. The marine microbial flocculant is produced through fermentation of Amylibacter lutimaris, has a flocculation effect in a wide pH and high salt concentration range, and has a wide application range. The invention provides a preparation method of the marine microbial flocculant. The marine microbial flocculant has a simple liquid medium preparation process, components have wide sources, and are easily available, and the microorganisms have low requirements for culture conditions and a short culture time; the microbial flocculant can be obtained only through fermentation after inoculation is conducted, the production process has few steps, and the dry flocculant product can be obtained through sedimentation, centrifugal separation and drying; and the purification process has simple operation, and the obtained dry flocculant product has the advantages of stable storage, convenient use and the like.

The invention belongs to the application field of agricultural microorganisms, and relates to a 2017-M2 bacterial strain containing a mannanase gene. The 2017-M2 bacterial strain can produce edible red pigment, mannanase and cellulase and can be obtained from soil through separation. The 2017-M2 bacterial strain containing the mannanase gene is Talaromyces atroroseus, and the sequence of the mannanase gene is shown in SEQ ID NO: 2. The similarity with known mannan beta-1,4-endonuclease gene is only 71%, and the 2017-M2 bacterial strain is a novel functional gene and has wide application value.The 2017-M2 bacterial strain grows well at 22DEG C-35 DEG C, produces the cellulase, the mannanase and amylase, does not produce protease, and has no bacteriostatic effect. The red pigment with a maximum light absorption value being 600 nm can be obtained in a PDA medium. The active gene can be expressed in Escherichia coli. The best induction conditions is 1<m>M IPTG 1.5 [mu]L, and induction iscarried out at 37 DEG C for 3 h.

The invention discloses a method for performing outdoor culture on isochrysis galbana parke suitable for southern climatic conditions. According to the method, an outdoor white plastic bucket is adopted for performing sealed aerated culture, and the culture appliance comprises the white plastic bucket, a transparent plastic cover covering the opening of the white plastic bucket, an air pipe, an air stone and a pumping device. The method is simple, feasible, low in requirement on culture conditions, low in culture cost and suitable for outdoor large-scale cultivation suitable for China southern climatic conditions, and the requirements of China southern marine economic shellfishes on important initial feed isochrysis galbana parke in breeding seasons are met.