48results about How to "Low requirements for cultivation conditions" patented technology

The invention belongs to the field of biological pesticides, and discloses a sugarcane endogenous Bacillus amyloliquefaciens CGB15 strain and application thereof. The Bacillus amyloliquefaciens CGB15 strain was deposited in the Guangdong Microbial Culture Collection Center on October 17, 2019, with the preservation number GDMCC No: 60742. The preservation address is: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City. The bacillus amyloliquefaciens CGB15 strain has a strong inhibitory effect on sugarcane whip smut, rice blast fungus, lychee perinospora, lychee anthracnose and banana wilt, and has excellent biological control effect. The preparation and use method of the microbial preparation obtained by the bacterial strain is simple, has broad-spectrum disease resistance, has little impact on the ecological environment, does not affect the quality of crops, and has excellent application prospects.

The invention belongs to the field of biopesticides, and discloses a strain of Pseudomonas fluorescens and its biological preparation and its application in preventing and treating whip smut of sugarcane. The strain of Pseudomonas fluorescens was named as Pseudomonas fluorescens HN58, and it was preserved in China Center for Type Culture Collection (CCTCC) on October 24, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC No: M2014511. The strain is isolated from sugarcane rhizosphere soil and can adapt to the local natural environment; it has a good inhibitory effect on sugarcane whip smut, and the source is environmentally friendly and non-toxic, and has little impact on the ecological environment; it has low requirements for culture conditions and has a good development and application prospects.

The invention relates to a method for dissolving mid-low-grade phosphate ore by utilizing activated sludge microbial flora. The method comprises the following steps: 1) performing enrichment culture on the activated sludge microbial flora, namely, performing the enrichment culture on the activated sludge microbial flora to obtain an activated sludge microbial flora enrichment culture solution; 2) performing hardening culture on the activated sludge microbial flora, namely, performing the hardening culture on the activated sludge microbial flora enrichment culture solution to obtain an activated sludge microbial flora hardening culture solution; 3) dissolving the mid-low-grade phosphate ore by the activated sludge microbial flora hardening culture solution, namely, adding a non-phosphorus culture medium and the mid-low-grade phosphate ore into a container, and then adding the activated sludge microbial flora hardening culture solution for culturing. The method provided by the invention is simple in process, low in culture condition requirements and low in production cost; the phosphate dissolving rate of the mid-low-grade phosphate ore reaches over 60 percent. By adopting the method, activated sludge can be changed from waste into treasure, and a positive effect on the utilization and treatment of municipal domestic wastewater is achieved.

The invention relates to the field of biotechnology and specifically discloses an alcaligenes faecalis strain Box-4 and its application. The alcaligenes faecalis strain Box-4 was collected in China Center for Type Culture Collection on March 12th, 2013, and the collection number is CCTCCNO:M2013090. The alcaligenes faecalis strain Box-4 is inoculated to a LB fluid medium with pH 7.0 and shake cultivation is carried out at 200r / min at 28 DEG C for 48h to obtain a biological agent. The biological agent has a strong effect of inhibiting Xanthomonas oryzae pv. oryzae and / or Xanthomonas oryzae pv. oryzicola and / or Ralstonia solanacearum, and sources of the biological agent are environmentally friendly, have no toxicity and have little influence on the ecological environment. In addition, the biological agent has low requirements on culture conditions, has a bacteriostatic effect on various pathogenic fungi, and has a good development and application prospect.

The invention discloses bacillus subtilis BCF-6, a biological agent prepared from the Bacillus subtilis BCF-6 as well as an application of the biological agent in prevention and control of Chinese orchid wilt. The bacillus subtilis BCF-6 is collected at China Center for Type Culture Collection on September 13, 2013, and the collection number of the bacillus subtilis BCF-6 is CCTCC No. M 2013420. The biological agent is prepared by inoculating the bacillus subtilis BCF-6 into a PD culture solution and carrying out shake cultivation at the temperature of 28 DEG C and the speed of 200r / min for 48 hours. The biological agent has a strong inhibiting effect on Chinese orchid wilt and little influence on ecological environments and is environment-friendly and non-toxic in source; in addition, the biological agent has low requirements on culture conditions, a strong inhibiting effect on pathogenic fungi and very good development and application prospect.

The invention discloses an entomopathogenic nematode symbiotic bacterial strain and its application for inhibiting various plant fungal diseases. The bacterial strain is a highly virulent strain NN6 of Pathogenobacter burgdorferi, and the preservation number is: CCTCC NO: M 2020097, April 29, 2020 Preserved in China Center for Type Culture Collection. The pathogenic bacteria burgdorferi NN6 of the present invention has stronger ability to inhibit the growth of Fusarium oxysporum watermelon-specific type and cucumber-specific type, Fusarium graminearum, Magnaporthe oryzae, Botrytis cinerea, Phytophthora capsici mycelium growth, and with With the increase of volume concentration, the antibacterial effect is remarkable. The bacterial strain also has the effect of inhibiting the germination of Fusarium spores, and the inhibitory effect is remarkable. The bacterium has the characteristics of low requirements for culture conditions, long duration, environmental protection and non-toxicity, etc. It is of great significance for protecting the ecological environment and improving the added value of agricultural products. In addition, it has the advantage of a wider antibacterial spectrum, which makes it very good. Market development and application prospects.

The invention belongs to the field of biological pesticide, and discloses a Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot. The Trichoderma asperellum is called Trichoderma asperellum PD-19, which is preserved in CCTCC on April 1st, 2013, and the address is Wuhan University, Wuhan city, China, and the preservation number in CCTCC is M2013113. The bacterial strain is obtained by separating from the cucumber rhizosphere soil, and can adapt to the local natural environment; the cucumber phytophthora pathogen has strong parasitic inhibition effect, and the source is environmental protection without toxicity and less ecological environment influence; the culture condition has a low requirement and a good exploitation and application prospect.

The invention belongs to the field of agricultural microorganism application, and relates to a 2017-M2 strain containing a mannanase gene. The strain can produce edible red pigment, mannanase and cellulase. isolated from the soil. It is Talaromyces atroroseus of the genus Talaromyces atroroseus, wherein the mannanase gene sequence is shown in SEQ ID NO:2. The similarity with the known mannan β-1,4-endonuclease gene is only 71%, which is a new functional gene and has wide application value. The strain grows well at 22°C-35°C, produces cellulase, mannanase and amylase, does not produce protease, and has no antibacterial effect. The red pigment with the maximum absorbance value at 600nm can be obtained in PDA medium. The active gene can be expressed in Escherichia coli. The optimal induction condition is 1.5μL of 1mM IPTG, induced for 3h at 37°C.

The invention discloses bacterium paenibacillus polymyma SHL-1 and an application thereof in preventing and controlling stalk rot of cymbidium sinense. The bacterium paenibacillus polymyma SHL-1 with a preservation number of CCTCCM2011441 is preserved in the China Center for Type Culture Collection on December 2, 2011. In the invention, the bacterium paenibacillus polymyma SHL-1 is inoculated to an LB (Langmuir-Blodgett) liquid culture medium with pH of 7.0, and is cultured for 48 hours in a oscillation way at 200r / min at a temperature of 28 DEG C, so that a biological preparation can be prepared. The biological preparation has a strong inhibition effect to the stalk rot of cymbidium sinense, and has environmentally-friendly non-toxic sources and small influence to biological environment;and furthermore, the biological preparation has low requirements to the culture condition, has an inhibition effect to various pathogenic fungi, and has good development and application prospects.

The invention relates to a method for dissolving low- and medium-grade phosphorite by using phosphorite pit water microbial flora. The method comprises the following steps: (1) enrichment cultivation of phosphorite pit water microbial flora: performing enrichment cultivation on the phosphorite pit water microbial flora to obtain a phosphorite pit water microbial flora enrichment culture solution; (2) dissolving of the low- and medium-grade phosphorite by using the phosphorite pit water microbial flora: adding a non-phosphorus culture medium and the low- and medium-grade phosphorite into a container, and then adding the phosphorite pit water microbial flora enrichment culture solution for cultivation. The method is simple technologically, low in requirement on cultivation condition and environment-friendly; the low- and medium-grade phosphorite solubilization efficiency is over 60 percent. The microbial flora is from phosphorite pit water, and is high in phosphorite environment adaptability, so that microbial acclimation cultivation is not needed.