48results about How to "Low requirements for cultivation conditions" patented technology

The invention belongs to the field of biopesticides, and discloses a strain of Pseudomonas fluorescens and its biological preparation and its application in preventing and treating whip smut of sugarcane. The strain of Pseudomonas fluorescens was named as Pseudomonas fluorescens HN58, and it was preserved in China Center for Type Culture Collection (CCTCC) on October 24, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC No: M2014511. The strain is isolated from sugarcane rhizosphere soil and can adapt to the local natural environment; it has a good inhibitory effect on sugarcane whip smut, and the source is environmentally friendly and non-toxic, and has little impact on the ecological environment; it has low requirements for culture conditions and has a good development and application prospects.

The invention relates to a method for dissolving mid-low-grade phosphate ore by utilizing activated sludge microbial flora. The method comprises the following steps: 1) performing enrichment culture on the activated sludge microbial flora, namely, performing the enrichment culture on the activated sludge microbial flora to obtain an activated sludge microbial flora enrichment culture solution; 2) performing hardening culture on the activated sludge microbial flora, namely, performing the hardening culture on the activated sludge microbial flora enrichment culture solution to obtain an activated sludge microbial flora hardening culture solution; 3) dissolving the mid-low-grade phosphate ore by the activated sludge microbial flora hardening culture solution, namely, adding a non-phosphorus culture medium and the mid-low-grade phosphate ore into a container, and then adding the activated sludge microbial flora hardening culture solution for culturing. The method provided by the invention is simple in process, low in culture condition requirements and low in production cost; the phosphate dissolving rate of the mid-low-grade phosphate ore reaches over 60 percent. By adopting the method, activated sludge can be changed from waste into treasure, and a positive effect on the utilization and treatment of municipal domestic wastewater is achieved.

The invention discloses bacillus subtilis BCF-6, a biological agent prepared from the Bacillus subtilis BCF-6 as well as an application of the biological agent in prevention and control of Chinese orchid wilt. The bacillus subtilis BCF-6 is collected at China Center for Type Culture Collection on September 13, 2013, and the collection number of the bacillus subtilis BCF-6 is CCTCC No. M 2013420. The biological agent is prepared by inoculating the bacillus subtilis BCF-6 into a PD culture solution and carrying out shake cultivation at the temperature of 28 DEG C and the speed of 200r / min for 48 hours. The biological agent has a strong inhibiting effect on Chinese orchid wilt and little influence on ecological environments and is environment-friendly and non-toxic in source; in addition, the biological agent has low requirements on culture conditions, a strong inhibiting effect on pathogenic fungi and very good development and application prospect.

The invention belongs to the field of agricultural microorganism application, and relates to a 2017-M2 strain containing a mannanase gene. The strain can produce edible red pigment, mannanase and cellulase. isolated from the soil. It is Talaromyces atroroseus of the genus Talaromyces atroroseus, wherein the mannanase gene sequence is shown in SEQ ID NO:2. The similarity with the known mannan β-1,4-endonuclease gene is only 71%, which is a new functional gene and has wide application value. The strain grows well at 22°C-35°C, produces cellulase, mannanase and amylase, does not produce protease, and has no antibacterial effect. The red pigment with the maximum absorbance value at 600nm can be obtained in PDA medium. The active gene can be expressed in Escherichia coli. The optimal induction condition is 1.5μL of 1mM IPTG, induced for 3h at 37°C.