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A strain of Alcaligenes faecalis bxo-4 and its application

A technology of Alcaligenes faecalis, bxo-4, applied in the application, bacteria, chemicals for biological control, etc., can solve the problems of too conservative and passive control methods, scarce biocontrol bacteria resources, etc., and achieve good development and application. Prospects, low requirements for cultivation conditions, and small impact on the ecological environment

Inactive Publication Date: 2016-09-14
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the prevention and treatment of crop diseases such as rice or peanuts, in the prior art, the selection of disease-resistant varieties is mainly used to reduce the incidence, but this prevention method is too conservative and passive.
Biological control is a relatively rapid development of plant disease control methods in recent years, but the resources of bio-control bacteria that can inhibit diseases are still relatively scarce. Therefore, it is an urgent need to develop bio-control bacteria with good antibacterial effect

Method used

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  • A strain of Alcaligenes faecalis bxo-4 and its application
  • A strain of Alcaligenes faecalis bxo-4 and its application
  • A strain of Alcaligenes faecalis bxo-4 and its application

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Experimental program
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Effect test

Embodiment 1

[0025] LB medium formula: tryptone 10 g, yeast extract powder 5 g, NaCl 10 g, agar powder 15 g, dilute to 1000 mL, pH 7.2. The above materials are LB liquid medium without agar powder.

[0026] The inventor collected paddy field soil from Zhongkai University of Agricultural Engineering on October 15, 2012, and separated strains by conventional dilution and separation methods. The specific steps were as follows: take 100 g of paddy field soil, fully dilute it with distilled water, and take 100 μl of soil droplets into the Sheng On a 10ml LB plate, pour 15ml of LB medium cooled to about 50°C, and shake the soil solution thoroughly, and then incubate at 26°C for 3 days, select a single colony, and perform three streaking dilutions and separation to obtain a single The colonies are stored at 4°C.

[0027] The strain was cultured on LB medium for 48 hours and observed, its single colony was round, white, with a smooth surface, opaque, slightly raised, and neat edges without wrinkles. ...

Embodiment 2

[0029] Example 2 Antibacterial spectrum of Alcaligenes facecalis Bio-4 culture medium

[0030] Taking the pathogens of several crop bacterial diseases as objects, the antibacterial spectrum of Alcaligenes facecalisBio-4 strain was determined by the inhibition zone method. Bacterial strains (antagonistic antibacterial Alcaligenes facecalis Bio-4 and pathogenic bacteria Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum) were inoculated into LB medium and cultured with shaking at 28 ℃, 200 r / min for 48 h. The three pathogenic bacteria strains were spread on the LB plate, and a sterile round paper sheet with a diameter of 6 mm was placed in the center of the plate, and 10 μL of the antagonistic bacteria Bio-4 was added to the round paper sheet. With the addition of LB liquid medium as a control, each treatment was repeated 3 times, cultured in a 28 ℃ constant temperature incubator for 4 days, and the inhibition radius was measured.

[0031] Table 1 Inhibit...

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Abstract

The invention relates to the field of biotechnology and specifically discloses an alcaligenes faecalis strain Box-4 and its application. The alcaligenes faecalis strain Box-4 was collected in China Center for Type Culture Collection on March 12th, 2013, and the collection number is CCTCCNO:M2013090. The alcaligenes faecalis strain Box-4 is inoculated to a LB fluid medium with pH 7.0 and shake cultivation is carried out at 200r / min at 28 DEG C for 48h to obtain a biological agent. The biological agent has a strong effect of inhibiting Xanthomonas oryzae pv. oryzae and / or Xanthomonas oryzae pv. oryzicola and / or Ralstonia solanacearum, and sources of the biological agent are environmentally friendly, have no toxicity and have little influence on the ecological environment. In addition, the biological agent has low requirements on culture conditions, has a bacteriostatic effect on various pathogenic fungi, and has a good development and application prospect.

Description

Technical field [0001] The present invention relates to the field of biotechnology, more specifically, to a strain of Alcaligenes faecalis Bxo-4 and its application. Background technique [0002] Rice (Oryza sativa) has a long history of cultivation in my country. Rice is native to tropical Asia. After being widely planted in China, it gradually spread to all over the world. Nearly half of the world's population feed on rice. It is the most important food crop in the world. With the development of rice, various diseases and insect pests have appeared constantly, becoming one of the limiting factors affecting rice production. Rice bacterial blight (Xanthomonas oryzae pv. oryzae) is an important disease in rice production. When the damage is serious, it can cause plant death and pose a serious threat to rice production. Rice bacterial streak disease (shortened thin streak disease) is also an important disease in rice production and belongs to the national agricultural plant qua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/05
Inventor 游春平刘凤权张云霞向梅梅肖爱萍黄礼雄张喆
Owner ZHONGKAI UNIV OF AGRI & ENG
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