Biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and application of biocontrol strain PNC25

A biocontrol strain, technology of Lychee Downy Phytophthora, applied in the fields of application, bacteria, biocide, etc., can solve the problems that have not yet been seen in the function of Acetyl Microbacterium to prevent and control Lychee Frost Blight, and achieve good development and application prospects, excellent control, and cultivation conditions less demanding effect

Active Publication Date: 2019-02-01
中国热带农业科学院广州实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective measures for the control of litchi frost blight are mainly chemical control. However, due to the environmental pollution caused by the extensive use of chemical pesticides and the problem of drug resistance of pathogens, the research and development of low-toxic, non-toxic The use of residual biogenic pesticides in the prevention and control of litchi diseases is of great significance for controlling the amount of pesticide residues, ensuring food safety and improving the international competitiveness of products
So far, there are no related reports about the function of Microbacterium acetylides in preventing and controlling frost blight of litchi

Method used

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  • Biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and application of biocontrol strain PNC25
  • Biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and application of biocontrol strain PNC25
  • Biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and application of biocontrol strain PNC25

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Source of the strain: PNC25 was isolated from the pulp of "Yuan Nuo" in a lychee orchard in Huilai, Jieyang City, Guangdong Province.

[0027] The collected samples were quickly put into numbered plastic bags and brought back to the laboratory for separation. Cut 3 g of pulp, soak in 1 mL of 1% sodium hypochlorite for 5 minutes, soak in 70% alcohol for 2 minutes, and wash with sterile water for 3 times. (Take 0.1mL of the last cleaning solution and apply R2A, or take 0.1mL of the last cleaning solution and add R2A 2 A culture solution, shake culture at 30°C, if it grows aseptically after 48 hours, it is considered that the surface is clean). Grind the surface-sterilized pulp with a sterilized mortar, add 3mL sterile water, filter with sterile cotton cloth, dilute 10 times with sterile water, take 0.1mL to dilute in turn, take 10 -4 、10 -5 、10 -6 Three gradients of 0.1mL coated with R 2 Plate A, cultivated at 28°C for 48 hours, selected plates with 50-300 colonies, ...

Embodiment 2

[0036] Embodiment 2 bacterial agent preparation

[0037] (1) Cultivation of seed bacteria liquid: inoculate PNC25 (GDMCC No.60108) into LB culture liquid, culture at 28°C and 200 rpm for 24 hours, and after 16 hours, take samples every 3 hours in the ultra-clean workbench and measure the OD value at 600nm During the measurement process, use the uninoculated culture solution to adjust to zero, until the OD value of the seed bacteria solution is between 0.5 and 0.8, the culture is ended, and the seed bacteria solution is obtained;

[0038] (2) Preparation of wet bacteria: Add the seed bacteria solution into the LB culture solution at a volume ratio of 1:500, shake and culture at 28°C and 200rpm for 24 hours to obtain a biocontrol agent, and the concentration of viable bacteria is 1×10 8 ~10 9 CFU / mL.

Embodiment 3

[0039] Example 3 Biocontrol bacteria PNC25 evaluates the indoor (LT, laboratory trial) disease prevention effect of litchi fruit / leaf

[0040] The healthy lychees picked in the field are processed as follows: A. Dilute the stock solution of biocontrol bacteria to a final concentration of 5*10 7 CFU / mL, spray fruit / dilute to final concentration 1*10 7 CFU / mL spray leaves; B. LB solution of the same dilution multiple. Set up 3 repetitions for each treatment, each repetition has 30 fruits / 5 branches, and the fruits / branches are placed in a fresh-keeping box (the bottom of the box is covered with sterilized filter paper, 18 cm in diameter, moistened with sterilized water, and the size of the fresh-keeping box is 323*220 *100mm, the manufacturer is Hualong Plastic Factory, Chancheng District, Foshan City). The bacterial solution and the control group were sprayed with a total of 300mL for each treatment (3 repetitions). 24hpt (hours post treatment) spray inoculation of pathogenic ...

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Abstract

The invention discloses a biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases and an application of the biocontrol strain PNC25. The biocontrol strain PNC25 for preventing and controlling litchi downy blight diseases refers to Exiguobacterium acetylicum, collected in the Guangdong Microbial Culture Collection Center, with the collection number of GDMCC No. 60108, onthe date of November 15th, 2016. The Exiguobacterium acetylicum can suppress the litchi downy blight diseases, and has excellent biocontrol effect on the litchi downy blight diseases on multiple experiment conditions. Besides the application in preventing and controlling the litchi downy blight diseases, the Exiguobacterium acetylicum has the good effect in keeping litchi fresh, and accordingly can be used for preparation of litchi preservatives.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol strain PNC25 for preventing and treating litchi frost blight and its application. Background technique [0002] Litchi (Litchi chinensis Sonn) is an important subtropical economic crop in the southern region. It is known as the "Chinese Precious Fruit" and has a high economic value. It is one of the most competitive fruits in the international market in my country. However, in the main producing areas of litchi in my country, downy mildew caused by Peronophythora litchii Chen ex Ko et al. is one of the most important diseases that cause litchi yield reduction. The prevalence of this disease has the characteristics of indirect outbreaks and is greatly affected by the climate. In a suitable year for the disease, it can cause more than 80% loss of litchi. At present, the effective measures for the control of litchi frost blight are mainly chemical control. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 郑丽吴健雄蒋仁娇
Owner 中国热带农业科学院广州实验站
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