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Method for preparing plant haploid embryo and haploid plant

A haploid and plant technology, applied in the field of plant cultivation, can solve the problems of harsh cultivation conditions, high cost, complicated procedures, etc., and achieve the effect of low cultivation conditions, low cost, and simple operation

Inactive Publication Date: 2008-01-16
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method of in vitro parthenogenesis using unpollinated ovaries or (and) ovules to obtain haploids in vitro has disadvantages such as complex procedures, harsh culture conditions, cumbersome operations, and high costs.

Method used

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  • Method for preparing plant haploid embryo and haploid plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of Haploid Embryos

[0042] 1, choose 20 well-grown loquat plants (kinds are all big five-star), be divided into 2 groups at random: 4 strains are control group, 16 strains are experimental group, before loquat is about to bloom 1-2 days, from each plant growth vigor On a good inflorescence, select 1 to 4 spherical flowers per inflorescence (the flowers in this period have not yet opened, and the pollen has not yet matured, so the pollination and fertilization process has not occurred), and all the remaining flowers are removed. Remove the selected spherical flowers, and use ophthalmic scissors to cut off 1 to 4 of the 5 stigmas (cut off 1 at random, or cut 2 to 3 stigmas at intervals, or keep only one in the center of the flower, Cut off 4).

[0043] 2. When the petals are open (2 to 3 days after removing the stigma), give the remaining 1 to 4 stigmas a sufficient amount of mixed pollen (Dawuxing: Longquan No. 1 = 1:1).

[0044] 3. Remove the fl...

Embodiment 2

[0049] Example 2 Preparation of Haploid Plants

[0050] The haploid embryos prepared from Group A in Example 1 were directly inoculated on sterilized MS medium respectively, and directly inoculated on the culture medium supplemented with 6-BA 0.5mg / L, NAA 0.1mg / L, IBA 0.1mg / L and 0.5% activated carbon on sterilized MS medium, cultured in the dark at 25±1°C for 15 days, 25±1°C, 18 hours in the light, 6 hours in the dark, and cultivated into seedlings under 1500lux light intensity (about one month after cultivation) .

[0051] After growing into a complete seedling, the haploid loquat is hardened to adapt to the external environment. The seedling hardening method is as follows: move the culture bottle to room temperature (15-25°C) for 2 days to allow the cultivated haploid seedlings to gradually adapt to changes in external light and temperature; then remove the sterile sealing film of the culture bottle to allow the outside Air enters the culture bottle, so that the haploid s...

Embodiment 3

[0053] Example 3 Preparation of Haploid Plants

[0054] The haploid embryos prepared by group B in Example 1 were directly inoculated on the sterilized MS medium, and directly inoculated on the medium added with 6-BA 0.3mg / L, NAA0.05mg / L, IBA0.05mg / L and Put 0.3% activated carbon on sterilized MS medium, culture in the dark at 25±1°C for 7 days, then culture at 25±1°C for 18 hours in the light, 6 hours in the dark, and 2000lux light intensity to grow into seedlings (after about one month of cultivation) .

[0055] After growing into a complete seedling, the haploid loquat is hardened to adapt to the external environment. The seedling hardening method is as follows: move the culture bottle to room temperature (15-25°C) for 3 days to allow the cultured haploid seedlings to gradually adapt to the changes in external light and temperature; then remove the sterile sealing film of the culture bottle to make the outside world The air enters the culture bottle, so that the haploid s...

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Abstract

The invention provides a method for preparing plant monoploid idiosome and monoploid plant, belonging to plant cultivating field, the method for preparing plant monoploid idiosome comprising the following steps: a. shearing off 1-3 column cap in the flower before flower blooming; b. carrying out artificial pollination for the left column cap when flower blooming; c. drawing the monoploid idiosome from the unfertilized blastocyst when the fruit is becoming mature. The method for preparing monoploid plant comprises the following steps: a. inoculating the prepared monoploid idiosome into the culture medium for culturing sprout; b. training sprout, replanting for culturing. The invention is characterized by the simple operation, no need special instrument, low cost, high success rate, suitable for field application and bright practical applying prospect.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for preparing plant haploid embryos and haploid plants. Background technique [0002] Individuals, tissues, or cells that only have gametophyte chromosome components are cultured in vitro to develop into complete plants. This plant with a single set of chromosomes is called a haploid plant (Haploid Plant). Haploid is of great significance in plant genetics and breeding: ①Inbred lines and clones of cross-pollinated crops can be quickly obtained through haploid culture and chromosome doubling. ②Create new germplasm resources through the variation in haploid culture. ③ Recessive mutations can be found quickly by mutagenic haploids. ④It plays an important role in the theoretical research of plant reproductive mechanism. For example, the aneuploid obtained by crossing haploid and diploid helps to solve some problems such as determination of linkage group, chromo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00A01H4/00A01G1/00C12N5/04
Inventor 王永清李俊强邓群仙侯春霞周兰英王小蓉陈红
Owner SICHUAN AGRI UNIV
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