Method for preparing triploid plants

A technology of triploid and plant, which is applied in the field of plant cultivation, can solve the problems such as the difficulty in preparing plant triploid plants, and achieve the effects of overcoming differences in plant species, wide application, and low requirements for equipment

Inactive Publication Date: 2012-07-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that it is difficult to prepare triploid plants in the prior art

Method used

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  • Method for preparing triploid plants
  • Method for preparing triploid plants
  • Method for preparing triploid plants

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Experimental program
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Effect test

Embodiment 1

[0031] The preparation of embodiment 1 small seed

[0032] 1. Select 20 well-grown loquat plants (the varieties are all big five-stars), and be randomly divided into 2 groups: 4 strains are the control group and 16 strains are the experimental group. Select 5-6 spherical flowers per inflorescence on a good inflorescence (the flowers in this period have not yet opened, and the pollen has not yet matured, so the pollination and fertilization process has not occurred), and all the remaining flowers are removed. Remove the selected spherical flowers, and use ophthalmic scissors to cut off 2 to 3 of the 5 stigmas (2 to 2 at random, or 2 to 3 at intervals of 1 stigma).

[0033] 2. When the petals are open (1 to 2 days after removing the stigma), give the remaining stigma a sufficient amount of mixed pollen (Dawuxing: Longquan No. 1 = 1:1).

[0034] 3. When the fruit develops to a diameter of about 1.5 cm, thin out the abnormally developed fruit, leaving 3 to 4 fruits on each ear, so ...

Embodiment 2 3

[0037] The preparation of embodiment 2 triploid plants

[0038] The small seeds prepared by group A in Example 1 were directly inoculated respectively on the sterilized MS medium with 30 g / L of sucrose, 0.1 mg / L of ZT, 0.1 mg / L of NAA and 0.005 g / L of colchicine , and cultured in the dark at 28±1°C for 10 days, and then cultivated into seedlings at 28±1°C, 16 hours in light, 8 hours in darkness, and 1000 lux light intensity (40 days in culture).

[0039] The small seeds of the control group were directly inoculated on the sterilized MS medium supplemented with 30 g / L sucrose, 0.5 mg / L 6-BA, 0.02 mg / L NAA, 0.02 mg / L IBA and 0.2% activated carbon, and placed in 28 Cultivate in the dark at ±1°C for 10 days, then at 25±1°C in the dark for 8 days, and then cultivate for one month under the light of 2000±500lux, 14-18h / d, to form seedlings.

[0040] After the seeds grow into complete seedlings, harden the seedlings to adapt to the external environment. The seedling hardening metho...

Embodiment 3 3

[0044] The preparation of embodiment 3 triploid plants

[0045] The small seeds prepared by group B in Example 1 were directly inoculated on sterilized MS medium respectively, and directly inoculated on sucrose 30g / L, ZT 0.1mg / L, NAA 0.1mg / L and colchicine 0.01 G / L sterilized MS medium, cultured in the dark at 28±1°C for 10 days, then cultured at 28±1°C, 16 hours in light, 8 hours in darkness, and 1000lux light intensity (40 days in culture) .

[0046] After growing into complete seedlings, harden the seedlings to adapt to the external environment. The seedling hardening method is as follows: move the culture bottle to room temperature (15-25°C) for 3 days, let the cultivated small seedlings gradually adapt to changes in external light and temperature; then remove the sterile sealing film of the culture bottle, and let the outside air After entering the culture bottle, the small seedlings can obtain resistance to microorganisms under normal natural conditions and adapt to ch...

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Abstract

The invention provides a method for preparing triploid plants, which belongs to the field of plant cultivation. The preparation method comprises the following steps: a, cutting 2 to 3 stigmas in a flower before a loquat blossoms; b, artificially pollinating reserved stigmas when petals open; c, taking out small seeds from an unfertilized embryo sac when loquat fruits are about to be mature; d, inoculating a culture medium with the small seeds to culture the small seeds into seedlings; and e, hardening, transplanting and culturing the seedlings to obtain plants. The method has the advantages of simple operation, no need for special instruments or equipment, low cost, high success rate, applicability to field promotion and good practical application prospects.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for preparing plant triploid plants. Background technique [0002] Loquat is a unique subtropical fruit tree native to my country. The fruit is on the market in the off-season at the turn of spring and summer. It is rich in nutrition and excellent in flavor, and is very popular among people. However, the fly in the ointment of loquat is that the seeds are many and large, often reaching about one-third of the weight of the fruit, resulting in less than 70% of the loquat fruit being edible. [0003] Cultivating seedless loquat varieties is a long-cherished wish of horticultural breeders. The triploid approach is the most ideal technical route for cultivating seedless varieties. Organisms whose somatic cells contain 3 chromosome sets are called triploids. In the breeding of horticultural plant varieties, triploid breeding has special significance, and can bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 王永清李俊强邓群仙吕秀兰严娟王小蓉
Owner SICHUAN AGRI UNIV
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