Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot

A technology for cucumber Phytophthora phytophthora and Trichoderma aculeatus, which is applied in the directions of chemicals for biological control, biocides, microorganism-based methods, etc., can solve problems such as threats to cucumber production safety, plant dieback, etc., and achieve good development Application prospects, low requirements for culture conditions, strong inhibitory effect

Active Publication Date: 2014-12-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Investigations in various cities and counties (districts) in Guangdong Province found that the disease can occur throughout the growth period, and when the damage is severe, it can lead to plant death. The incidence rate of some fields is as high as 40%, which poses a serious threat to the safe production of cucumbers.

Method used

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  • Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot
  • Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot
  • Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation, purification and preservation of Trichoderma aculeatus

[0026] (1) Configuration of PDA medium: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for 0.5h, filter with gauze, add 20g of glucose and 20g of agar, fully dissolve, filter with hot gauze, and sterilize at 121°C After 20 minutes, divide into petri dishes and store for later use after cooling.

[0027] (2) Isolation and purification of Trichoderma aculeatus:

[0028] Weigh 50g of fresh cucumber rhizosphere soil sample (this soil sample is a diseased cucumber rhizosphere soil sample, taken from the suburbs of Shenzhen) and add it into a 1000mL triangular flask filled with 450mL of 0.9wt% normal saline. Shake on a shaker for about 20 minutes to prepare a dilution factor of 10 -1 soil dilution; take 1mL10 -1 The soil dilution was added to a test tube filled with 9mL of 0.9% normal saline, and the dilution factor was prepared to be 10 -2 The soil dilution so...

Embodiment 2

[0033] Embodiment 2: confrontation culture method measures the activity of Trichoderma aculeatus

[0034] (1) The preparation of PDA medium is the same as in Example 1.

[0035] (2) Preparation of PD culture medium: Weigh 200g of potatoes and cut into small pieces, add water and boil for 20-30min until it can be punctured by a glass rod, then filter with eight layers of gauze; heat the filtrate, add 20g of glucose, stir evenly, and cool slightly Then make up the water to 1000mL, divide into Erlenmeyer flasks (100mL culture solution in each bottle), stopper, bandage, sterilize at 121°C for 20min, cool and store for later use.

[0036] (3) Preparation of Trichoderma aculeatus fungus cake: Trichoderma aculeatus was inoculated on a PDA medium plate for 3 days of activation and cultured, and punched with a 5 mm diameter punch to make a fungus cake.

[0037] (4) Preparation of Trichoderma aculeatus culture filtrate: Take the Trichoderma aculeatus cake prepared in step (3) and inser...

Embodiment 3

[0042] Embodiment 3: Trichoderma aculeatus controls cucumber Phytophthora

[0043] (1) The preparation of PDA medium is the same as in Example 1. The preparation of PD culture medium is the same as that in Example 2.

[0044] (2) The preparation of Trichoderma aculeatus cake and the cultivation of Phytophthora cucumber are the same as in Example 2.

[0045] (3) Preparation of Trichoderma aculeatus biological preparation: Take the bacteria cake prepared in 5 steps (1) into a 250mL Erlenmeyer flask containing 100mL PD culture solution, place it at 27°C, 200rpm, and culture it on a shaker for 2 days, use The concentration of sterile water is about 10 8 Individual / mL thalli suspension, promptly obtains Trichoderma aculeatus strain PD-19 biological agent.

[0046] (4) 1.3kg of sterilized soil is placed in the flowerpot, and 10mL of the Trichoderma aculeatus biological agent prepared in step (3) is mixed with the soil (spore concentration is 1*10 6 per g soil) for 7 days, then m...

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Abstract

The invention belongs to the field of biological pesticide, and discloses a Trichoderma asperellum and biological preparation and application in controlling and preventing cucumber phytophthora root rot. The Trichoderma asperellum is called Trichoderma asperellum PD-19, which is preserved in CCTCC on April 1st, 2013, and the address is Wuhan University, Wuhan city, China, and the preservation number in CCTCC is M2013113. The bacterial strain is obtained by separating from the cucumber rhizosphere soil, and can adapt to the local natural environment; the cucumber phytophthora pathogen has strong parasitic inhibition effect, and the source is environmental protection without toxicity and less ecological environment influence; the culture condition has a low requirement and a good exploitation and application prospect.

Description

technical field [0001] The invention belongs to the field of biopesticides, and in particular relates to a strain of Trichoderma aculeatus and its biological preparation and its application in preventing and treating cucumber phytophthora. Background technique [0002] Cucumber (Cucumis sativas L.) is an important melon vegetable crop, which is widely planted in my country and has extremely high economic value. In recent years, with continuous cropping and expansion of cucumber planting area, cucumber Phytophthora melonis Katsura has become one of the limiting factors affecting cucumber production. Investigations in various cities and counties (districts) in Guangdong Province found that the disease can occur throughout the growth period, and when the damage is severe, it can lead to plant death. The incidence rate of some fields is as high as 40%, which poses a serious threat to the safe production of cucumbers. At present, the control of this disease in production is main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 习平根姜子德袁玉花李敏慧骆晶晶李升浩
Owner SOUTH CHINA AGRI UNIV
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