A kind of bacterium bacillus subtilis BCF-6 and the biological preparation and application thereof

A technology for biological agents and bacteria, applied in the directions of biocides, chemicals for biological control, bacteria, etc., can solve problems such as difficulties in prevention and control in production, achieve good development and application prospects, low requirements for cultivation conditions, and ecological environment. low impact effect

Active Publication Date: 2016-04-20
中科生物技术转移(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the disease is a soil-borne disease caused by a sickle, it is extremely difficult to prevent and control it in production

Method used

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  • A kind of bacterium bacillus subtilis BCF-6 and the biological preparation and application thereof
  • A kind of bacterium bacillus subtilis BCF-6 and the biological preparation and application thereof
  • A kind of bacterium bacillus subtilis BCF-6 and the biological preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1B

[0023] Example 1 Bacillus subtilis Activity of BCF-6 Culture Solution against Fusarium wilt

[0024] The BCF-6 strain was isolated from the pseudobulb of Chrysanthemum chinensis by conventional tissue separation method. The strain was observed after being cultivated on PDA medium for 48 hours, and its single colony was round, white, smooth, opaque, raised, and with neat edges. The strain was analyzed by 16SrDNA sequence, and its sequence was the same as that in GenBank Bacillus subtilis (Accession numbers are JX524224 and HQ441254) have a homology of 99%; analysis by the BIOLOG bacteria identification system software shows that the BCF-6 strain is only related to the Bacillus subtilis A good match, its similarity Similarity (SIM) is 0.610.

[0025] The activity of the live bacteria of the strain and the culture filtrate of the strain was determined by the plate confrontation method. Bacterial strains were inoculated in PD culture medium, and cultured at 28°C and 200r / min...

Embodiment 2B

[0026] Example 2 Bacillus subtilis Antibacterial Spectrum of BCF-6 Culture Solution

[0027] Taking several flower fungal disease pathogens as objects, the inhibition zone method was used to determine the Bacillus subtilis Antibacterial spectrum of the BCF-6 strain. Bacterial strains were inoculated in PD culture medium, and cultured at 28°C and 200r / min for 48h with shaking. Use a hole puncher with an inner diameter of 0.6 mm to punch a hole on the PDA medium, add 60 μL of bacterial solution into the hole, and inoculate chrysanthemum (Xiufengbai) wilt fungus, white palm brown rot fungus and Dahua at a distance of 3 cm from the hole. Cymbidium leaf spot fungus. Taking the addition of PD culture medium as a control, each treatment was repeated 3 times, and cultured in a constant temperature incubator at 28°C. When the diameter of the control colony reached 6cm, the width of the inhibition zone was measured. The results show: Bacillus subtilis BCF-6 has a good inhibitory ef...

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Abstract

The invention discloses bacillus subtilis BCF-6, a biological agent prepared from the Bacillus subtilis BCF-6 as well as an application of the biological agent in prevention and control of Chinese orchid wilt. The bacillus subtilis BCF-6 is collected at China Center for Type Culture Collection on September 13, 2013, and the collection number of the bacillus subtilis BCF-6 is CCTCC No. M 2013420. The biological agent is prepared by inoculating the bacillus subtilis BCF-6 into a PD culture solution and carrying out shake cultivation at the temperature of 28 DEG C and the speed of 200r / min for 48 hours. The biological agent has a strong inhibiting effect on Chinese orchid wilt and little influence on ecological environments and is environment-friendly and non-toxic in source; in addition, the biological agent has low requirements on culture conditions, a strong inhibiting effect on pathogenic fungi and very good development and application prospect.

Description

technical field [0001] The present invention relates to the field of biopesticides, more specifically, to a bacterial Bacillus subtilis BCF-6 and its biological preparation and application. Background technique [0002] Guolan ( Cymbidium spp.) has a long history of cultivation in my country, mainly distributed in Guangdong, Fujian, Taiwan, Zhejiang, Jiangsu, Sichuan, Yunnan and other places. Traditional Chinese orchids mainly include Chunlan ( Cymbidium goeringii ), Cymbidium ( C.faberi ), Jianlan ( C. ensifolium ),Cymbidium( C. sinense ) and Hanlan ( C. kanran ) and other 5 categories. In recent years, with the gradual expansion of the cultivation area of ​​orchids, diseases have become the main factors restricting the development of production, among which Fusarium wilt is particularly prominent, known as the number one killer in the production of orchids. Fusarium wilt is caused by Fusarium ( Fusarium oxysporum ) caused by systemic diseases, the pathogeni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 张云霞向梅梅孙书荃游春平施祖荣黄江华
Owner 中科生物技术转移(深圳)有限公司
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