Culture base for inducing fusariumgraminearum schw to massively generate conidium

A technology for Fusarium graminearum and culture medium, which is applied to the field of spore-producing medium for plant pathogenic fungi, can solve the problems of low number of conidia and difficulty in identifying pathogenic bacteria, etc., and achieves the effects of low cost, space saving and wide application.

Pending Publication Date: 2017-02-22
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problems that the number of Fusarium graminearum conidia produced in the current culture medium is small, and it is difficult to meet the needs of pathogenic bacteria identification, strain preservation, and inoculation, the purpose of the present invention is to provide a kind of mung bean powder or mung bean dregs to induce the production of Fusarium graminearum. Application on spores

Method used

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  • Culture base for inducing fusariumgraminearum schw to massively generate conidium
  • Culture base for inducing fusariumgraminearum schw to massively generate conidium
  • Culture base for inducing fusariumgraminearum schw to massively generate conidium

Examples

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Effect test

Embodiment 1

[0034] Contrasting test of sporulation of Fusarium graminearum on different mediums of embodiment 1

[0035] (1) The tested Fusarium graminearum strain 1449: It is a wheat scab disease sample collected from Zhao County, Shijiazhuang, Hebei Province in 2014. It was isolated according to the isolation method of pathogenic fungi in the plant disease research method, and the initial isolate was subjected to monospore The pure culture strain was obtained by isolation and culture, and was identified as Fusarium graminearum Schw. by morphological and molecular biology methods. The strain was preserved in the Fungal Disease Laboratory of Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences.

[0036] (2) Test medium and its preparation:

[0037] (1) PDA medium: cut 200g of washed and peeled potatoes into pieces, add 1000mL of water, cook in boiling water for half an hour, filter with gauze, take potato juice, add water to make up 1000mL, add 20g of glucose and...

Embodiment 2

[0050] Example 2 Contrast test of mung bean flour with different particle sizes inducing sporulation of Fusarium graminearum

[0051] (1) Test medium:

[0052] Preparation of 2% mung bean flour medium with different particle sizes: crush fresh mung beans with a cooking machine, and then pass through 10 mesh, 20 mesh, 30 mesh, and 60 mesh sieves to obtain particle sizes of <1770 μm, 830 μm, and 60 μm respectively. 1770 μm, 550~830 μm, 250~550 μm and 0~250 μm five kinds of mung bean flour with different particle sizes, prepare 2% mung bean flour medium with different particle sizes according to the method of Example 1.

[0053] (2) Test method:

[0054] Pick the large conidia heap that produces on mung bean flour medium or mung bean dregs medium in embodiment 1, adjust spore suspension concentration to 5 * 10 with sterilized water 4 Conidia / mL, take 1 mL of the spore suspension and apply it on the 2% mung bean flour medium plate with different particle sizes prepared in the st...

Embodiment 3

[0058] Example 3 Contrast Test of Fusarium graminearum Sporulation Induced by Culture Medium with Different Mung Bean Powder Concentrations

[0059] (1) Test medium:

[0060] 2%~8% mung bean powder culture medium: the preparation method is the same as that in Example 1, and the addition amount of mung bean powder is respectively 20g, 30g, 40g, 50g, 60g, 70g and 80g.

[0061] (2) Test method:

[0062] Pick the large conidia heap that produces on mung bean flour medium or mung bean dregs medium in embodiment 1, adjust spore suspension concentration to 5 * 10 with sterilized water 4 Conidia / mL, take 1 mL of the spore suspension and apply it on the 2%-8% mung bean flour medium plate prepared in the step (1) after standing and drying, and culture it under natural light at 16-22°C for 10 days. The large conidia on the petri dish were washed with sterile water, the number of conidia was counted under a microscope, and the difference was analyzed significantly.

[0063] Result (see...

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Abstract

The invention discloses an application of green gram starch and green bean dregs in inducing fusariumgraminearum schw to massively generate conidium, and also discloses a corresponding bean dregs culture base or a green bean dregs culture base; the culture base is prepared from, by weight, 20-80 g of green gram starch or green bean dregs, 15-25 g of agar powder, and 1000 ml of distilled water. The invention further discloses a method for inducing fusariumgraminearum schw to massively generate conidium. The fusariumgraminearum schw has big conidium output on the green gram starch or green bean dregs base; the conidium output per cm2 is up to 107 pieces; moreover, massive large conidium piles can be generated; secondly, the method is simple and high low requirement on culture condition, and needs not the additional near ultraviolet lamp induction; besides, the culture base is simple in component, easy to obtain raw materials and low in cost.

Description

technical field [0001] The invention belongs to a plant pathogenic fungus sporulation culture medium, in particular to the use of mung bean flour in inducing sporulation of Fusarium graminearum; and also relates to a culture medium and method for inducing Fusarium graminearum to produce conidia in large quantities. Background technique [0002] Fusarium graminearum Schw. is an important pathogen widely distributed in the world. It has a wide range of hosts and can infect small grain cereal crops (including barley, wheat, rye, oats and triticale), corn, rice , sorghum and a variety of weeds, causing a variety of different diseases at different stages of the crop. Among them, wheat head blight, root and stem rot, corn ear and stem rot are mainly caused in food crops. Fusarium graminearum can not only cause a variety of crop diseases and cause yield loss and grain quality decline, but more seriously, Fusarium graminearum can produce a variety of toxins that can cause acute or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/77
CPCC12N3/00C12N1/14
Inventor 纪莉景孔令晓栗秋生王亚娇李聪聪
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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