33results about How to "Genetic manipulation is simple" patented technology

The invention provides EV71virus-like particles as well as preparation method and application of the EV71virus-like particles. The method comprises the following steps of: connecting a P1 protein gene and 3CD protease gene of an EV71 virus with predicated mean vote (PMV) plasmid to build PMV-P1-3CD recombinant expression plasmid; turning into hansenula polymorpha AU-0501 expression bacterial strain to obtain an AU-PMV-P1-3CD recombinant expression bacterial strain; fermenting and culturing the recombinant expression bacteria strain and performing inducible expression on EV71 virus-like particle proteins by using methanol; centrifugally gathering thallus to perform homogenate crushing at high pressure; and purifying the supernate through ion-exchange column chromatography, hydrophobic chromatography and sieve chromatography to obtain the EV71virus-like particles. EV71virus-like particle vaccines provided by the invention has high immunogenicity, security, immunological characteristics and biological activity; and the EV71virus-like particles can be prepared in a large scale and purified and used for preparing vaccines for preventing EV71 infection, and has good economic value and application prospect.

The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming a Hansenula polymorpha AU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.

The invention discloses a ship branch pipeline path planning method. The method comprises the steps of on the basis of simplifying a device ad a pipeline model, establishing a network graph between two points referring to a visible graph method in mobile robot path planning; setting branch pipelines containing N connection points, establishing the network graph between every two points of the N connection points, and combining update data information, thereby obtaining the total network graphs among the N points; establishing a population based on a Steiner point genetic algorithm, setting genetic algorithm parameters and then starting iteration to carry out optimization; determining the equivalent lengths of the pipelines and endowing different weight coefficients to different pipelines; evaluating the fitness value of each chromosome by use of a distance heuristic algorithm; judging whether set irritation times arrives or not; outputting optimum paths, finishing path planning, updating pipeline code information in a storage document, and realizing three-dimensional visualization of the ship branch pipelines through combination of three-dimensional design software. The method has very high search efficiency and can satisfy the practical demands of ship branch pipeline path planning better. The layout problem of the branch pipelines is solved.