Pleiomorphic saccharomyces hansenii expression recombination cholere toxin B subunit gene and its application

A technology of Hansenula polymorpha and cholera toxin, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as high cost, inability to industrialize, and pollution of CTA.

Inactive Publication Date: 2004-07-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are two methods to obtain CTB: one method is to isolate it from natural Vibrio cholerae, but this method has the risk of contaminating CTA and cannot be industrialized
Another method is to use gen

Method used

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  • Pleiomorphic saccharomyces hansenii expression recombination cholere toxin B subunit gene and its application
  • Pleiomorphic saccharomyces hansenii expression recombination cholere toxin B subunit gene and its application
  • Pleiomorphic saccharomyces hansenii expression recombination cholere toxin B subunit gene and its application

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[0022] Example

[0023] 1. Establishment of Hansenula high-expressed gene codon usage

[0024] Log in to the Genbank website http: / / www.ncbi.nlm.nih.gov The Hansenulaporlymorpha DNA sequence was retrieved, and the working draft and other non-essential sequences were excluded. After downloading to the local hard disk, use DNAStar software to remove the 5'and 3'untranslated regions of each sequence to obtain the coding sequence (CDS), and then use the DNAStar software MegAlign program to draw the evolutionary tree of the ORF (open reading frame) of all genes, and save the file As hansenula.pau. Then log in http: / / bioweb.pasteur.fr / seqanal / interfaces / codonw.html On the website, enter the entrance of the Codonw program, transfer the hansenula.pau file, and use the Codon W program to perform correspondence analysis (CA). The program automatically classifies Hansenula high-expressed genes and low-expressed genes. The number of samples of high-expressed genes and low-expressed genes a...

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Abstract

A polymorphous Hanson yeast expressed recombinant cholera toxin B subunit gene (CTB) is disclosed. In order to increase the expression of CTB in Hanson yeast, the coding gene of CTB is redesigned according to the application method of codon for the high-expression gene of Hanson yeast. The expression product can be used for preventing the diarrhea caused by cholera ribro and as the immune vaccine.

Description

technical field [0001] The invention belongs to the field of protein expression genetic engineering, and in particular relates to the use of a recombinant cholera toxin CTB subunit gene and the use of Hansenula polymorpha containing the gene as a cell factory to efficiently express CTB and the use of CTB and its fusion Application of protein in preparation of cholera vaccine and adjuvant. Background technique [0002] Cholera (Cholera) is one of the seven major epidemics in the world. It is designated as a Class A infectious disease in my country. It is an intestinal infectious disease caused by Vibrio cholerae (Vibrio cholerae), which causes acute diarrhea in humans and mammals during the onset , will soon die if not treated in time. Cholera toxin (CT) is a heat-labile enterotoxin secreted by Vibrio cholerae. The toxicity of CT is very strong, and humans are especially sensitive to CT, and 8 μg of CT can cause severe diarrhea. CT consists of two subunits, one A subunit (C...

Claims

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Application Information

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IPC IPC(8): A61K39/106A61K39/39C07K14/28C12N1/19C12N15/31C12N15/62C12N15/81C12P21/02
Inventor 邱并生宋厚辉李勇
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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