4 results about "Cholera Toxin B Subunit" patented technology

The invention relates to lipid nanoparticles containing an adjuvant for mucosal administration and a preparation method of the lipid nanoparticles. The lipid nanoparticles comprise total lipid, an adjuvant and an active ingredient, wherein the total lipid is composed of cationic lipid C12-200, non-cationic phospholipid, cholesterol and a derivative thereof, and pegylated lipid; wherein the adjuvant is selected from at least one of AS03, MF59, monophosphoryl lipid A, miniaturized monophosphoryl lipid A, ophiopogonin D, saponin QS21, QS7, QS17, QS18, CpG oligonucleotide, 2 '3'-ring GMP-AMP, 3 '3'-ring GMP-AMP, alpha GC, Trehalose-6, 6-dibehenate, cholera toxin B subunit and flagellin.

This invention discloses a method for rapidly constructing a complex of cholera toxin B subunit (CTB) and antigen. The vaccine complex includes a CTB-SpyTag fusion protein and at least one SpyCatcher-antigen fusion protein. It also includes a preparation method for rapidly preparing a CTB5-antigen complex by mixing CTB-SpyTag with the SpyCatcher-antigen. This invention directs the antigen to the side of the CTB pentamer that does not participate in GM1 binding, avoiding steric hindrance interference. It utilizes the SpyTag / SpyCatcher covalent isopeptide bond to obtain highly stable antigen display, reducing antigen shedding during subsequent preparation and storage. It also possesses mucosal targeting and multivalent presentation functions, providing a universal platform technology for rapidly constructing and validating broad-spectrum, combined, or personalized mucosal vaccines.

The disclosure provides, in various embodiments, compositions, for example, powders and pharmaceutical compositions, comprising a spray-dried formulation of a cholera toxin B subunit variant and a saccharide excipient. The disclosure also provides, in various embodiments, methods of making said compositions and methods of treating a disease or enhancing wound healing using said compositions. The disclosure further provides, in various embodiments, liquid compositions comprising a cholera toxin B subunit variant and mannitol.

The invention provides a fusion protein used as a retrograde tracer and a preparation method of the fusion protein. The fusion protein comprises cholera toxin B subunit protein (CTB), mbojin fluorescent protein and a linker used for connecting the cholera toxin B subunit protein and the mbojin fluorescent protein. The fusion protein is a protein (called CTBPro for short) after fusion of CTB and mbaojin, and shows remarkable fluorescence intensity, strong light stability and compatibility with multiple experimental modes. Importantly, the CTBPro can realize accurate retrograde tracing in the central and peripheral nervous systems, so that the neuron morphology is visualized with higher definition and details.