Asia 1-type FMDV compound multiepitope

A multi-epitope, antigenic epitope technology, applied in recombinant DNA technology, peptide source, antibody medical components, etc., can solve the problem of time-consuming, laborious, difficult to screen dominant epitopes, etc., and achieve the effect of high antigenicity

Inactive Publication Date: 2010-09-22
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large number of antigenic epitopes, it is time-consuming and labor-intensive to screen

Method used

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  • Asia 1-type FMDV compound multiepitope
  • Asia 1-type FMDV compound multiepitope
  • Asia 1-type FMDV compound multiepitope

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Construction of Asia 1 type FMDV composite multi-epitope

[0034] Based on a detailed understanding of the genome structure of Asia 1 type FMDV and its antigenic epitopes closely related to immunity, the popular strains of Asia 1 type FMDV in China were selected, combined with a professional network server (CTL Pred: http: / / www.imtech .res.in / cgibin / ctlpred / ctlpred.pl, Bce Pred: http: / / www.imtech.res.in / raghaVa / bcepred and Harvard: http: / / bio.dfci.harvard.edu / Tools / antigenic. pl) The epitope prediction results are adjusted so that the obtained antigenic active region is located in the intersection region of multiple epitopes as much as possible. A new artificial multi-epitope sequence is formed by linking selected antigenic active regions with small molecular flexible amino acids. Carry out whole gene chemical synthesis. Using molecular biology methods, the cholera toxin B subunit with a Linker at the end and the helper T lymphocyte epitope were linked to th...

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Abstract

The invention provides an Asia 1-type FMDV compound multiepitope which adopts an Asia 1-type FMDV capsid protein VP1 antigen activity area as the basic skeleton, and comprises the epitope of Asia 1/JL/05 plant VP1, the epitope of the IND 63/72 plant VP1, the epitope of YNBS/58 plant VP1, cholera toxin B subunit and helper T lymphocyte epitope. The compound multiepitope can be used for preparing Asia 1-type FMDV subunit vaccine, nucleic acid vaccine, synthetic peptide vaccine, live vector vaccine and a plurality of genetic engineering vaccines.

Description

technical field [0001] The present invention relates to a kind of Asia 1 type FMDV compound multi-epitope, particularly relate to a kind of Asia 1 type FMDV compound multi-epitope (protein) with the Asia 1 type FMDV capsid protein VP1 antigen active region as the backbone, the present invention also relates to The polynucleotide encoding the compound polyepitope (protein), and the genetic engineering vaccine constructed by using the compound polyepitope. Background technique [0002] Foot-and-mouth disease (Foot-and-Mouth Disease, FMD) is caused by Foot-and-Mouth Disease Virus (Foot-and-Mouth Disease Virus, FMDV) and is an acute, febrile, Highly contagious infectious disease. The disease has the characteristics of rapid spread, wide host range, high incidence rate, and wide prevalence. Therefore, it is designated as one of the must-report infectious diseases by the World Organization for Animal Health (OIE), and it is also listed as a first-class infectious disease in my co...

Claims

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Application Information

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IPC IPC(8): C07K14/09C12N15/42C12N15/63A61K39/135A61P31/14
Inventor 金宁一胡博李昌鲁会军李霄田明尧金扩世朱战波杜寿文
Owner JILIN UNIV
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