Novel helicobacter pylori multiepitope vaccine and preparation method thereof

A Helicobacter pylori, multi-epitope technology, applied in the field of biomedicine, can solve problems such as the increase of Hp drug-resistant strains, toxic and side effects, and long treatment cycles
CN102178941AInactive Publication Date: 2011-09-14CHINA PHARM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA PHARM UNIV
Publication Date
2011-09-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a helicobacter pylori multiepitope vaccine. The active ingredient of the helicobacter pylori multiepitope vaccine is a piece of polypeptide; and the helicobacter pylori multiepitope vaccine mainly comprises a multi-copy body of Th and B cell antigen epitopes of helicobacter pylori urease A and B bi-subunit and a mucosal immune adjuvant of a cholera toxin B subunit. The preparation method mainly comprises the following steps of: synthesizing an artificial gene by using a gene synthesis technology, wherein the artificial gene comprises a gene sequence of the multi-copy body of the Th and B cell antigen epitopes of the helicobacter pylori urease A and B bi-subunit; coupling the artificial gene with the gene sequence of the cholera toxin B subunit to form a fusion gene;and expressing the fusion gene by using an escherichia coli prokaryotic expression system, and performing protein purification to obtain the helicobacter pylori multiepitope vaccine. The helicobacterpylori multiepitope vaccine can induce an organism to generate T cell immune response and high-titred specific antibody humoral immune response aiming at the urease A and B bi-subunit, and can be used for preventing and treating helicobacter pylori infection related diseases.
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Description

Technical field

[0001] The invention belongs to the field of biomedicine, and specifically relates to a novel Helicobacter pylori multi-epitope vaccine and its preparation method and application. Background technique

[0002] Helicobacter pylori (Hp) is an important pathogenic factor of chronic gastritis, peptic ulcer and duodenal ulcer, and is closely related to gastric cancer. The World Health Organization (WHO) has listed Hp as a Class I carcinogen. The infection rate of Helicobacter pylori is very high, the Hp infection rate of the world population exceeds 50%, and the Hp infection rate of the Chinese population is 58.07%, showing family clustering, and the situation is more severe. The current treatment of Hp infection is mainly based on "triple" drug therapy, the main components of which are proton pump inhibitors, antibiotics and bismuth agents. The "triple" drug therapy has many disadvantages: (1) The widespread use of antibiotics has led to an increasing number of resis...

Claims

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